OPTN recruitment to a Golgi-proximal compartment regulates immune signalling and cytokine secretion

Optineurin (OPTN) is a multifunctional protein involved in autophagy, secretion as well as NF-κB and IRF3 signalling and OPTN mutations are associated with several human diseases. Here we show that, in response to viral RNA, OPTN translocates to foci in the perinuclear region, where it negatively regulates NF-κB and IRF3 signalling pathways and downstream pro-inflammatory cytokine secretion. These OPTN foci consist of a tight cluster of small membrane vesicles, which are positive for ATG9A. Disease mutations linked to POAG cause aberrant foci formation in the absence of stimuli, which correlates with the ability of OPTN to inhibit signalling. Using proximity labelling proteomics, we identify the LUBAC complex, CYLD and TBK1 as part of the OPTN interactome and show that these proteins are recruited to this OPTN-positive perinuclear compartment. Our work uncovers a crucial role for OPTN in dampening NF-κB and IRF3 signalling through the sequestration of LUBAC and other positive regulators in this viral RNA-induced compartment leading to altered pro-inflammatory cytokine secretion

Optical amplitude modulation extinction by a deep saturated ultra-long semiconductor optical amplifier

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The recovery of an optical carrier with the deletion of its amplitude modulation is introduced using a deeply saturated ultra-long semiconductor optical amplifier (UL-SOA). The experimental results were achieved for input signal bit rates up to 12.5 Gbps with high extinction ratio (up to 13.9 dB). The influence of parameters such as UL-SOA bias current, optical bandwidth, signal input power, modulation depth and bit rate are analyzed including the carrier spectral broadening effects due to the self-phase modulation effect. (C) 2010 Optical Society of America18262729827305Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)CEPOF - Research Center in PhotonicsFINEP (Financiadora de Estudos e Projetos)Fotonicom (Instituto Nacional de Ciencia e Tecnologia Optica para Comunicacoes Opticas)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah

Background: Wild, synanthropic and domestic mammals act as hosts and/or reservoirs of several Leishmania spp. Studies on possible reservoirs of Leishmania in different areas are fundamental to understand host-parasite interactions and develop strategies for the surveillance and control of leishmaniasis. In the present study, we evaluated the Leishmania spp. occurrence in mammals in two conservation units and their surroundings in Brasília, Federal District (FD), Brazil. Methods: Small mammals were captured in Brasília National Park (BNP) and Contagem Biological Reserve (CBR) and dogs were sampled in residential areas in their vicinity. Skin and blood samples were evaluated by PCR using different molecular markers (D7 24Sα rRNA and rDNA ITS1). Leishmania species were identified by sequencing of PCR products. Dog blood samples were subjected to the rapid immunochromatographic test (DPP) for detection of anti-Leishmania infantum antibodies. Results: 179 wild mammals were studied and 20.1% had Leishmania DNA successfully detected in at least one sample. Six mammal species were considered infected: Clyomys laticeps, Necromys lasiurus, Nectomys rattus, Rhipidomys macrurus, Didelphis albiventris and Gracilinanus agilis. No significant difference, comparing the proportion of individuals with Leishmania spp., was observed between the sampled areas and wild mammal species. Most of the positive samples were collected from the rodent N. lasiurus, infected by L. amazonensis or L. braziliensis. Moreover, infections by Trypanosoma spp. were detected in N. lasiurus and G. agilis. All 19 dog samples were positive by DPP; however, only three (15.8%) were confirmed by PCR assays. DNA sequences of ITS1 dog amplicons showed 100% identity with L. infantum sequence. Conclusions: The results suggest the participation of six species of wild mammals in the enzootic transmission of Leishmania spp. in FD. This is the first report of L. amazonensis in N. lasiurus

OPTICAL CARRIER SINUSOIDAL MODULATION SUPPRESSED BY ULTRA-LONG SEMICONDUCTOR OPTICAL AMPLIFIER GAIN SATURATION

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The amplitude modulated sinusoidal pattern over an optical carrier is suppressed by a deeply saturated ultra-long semiconductor optical amplifier. The extinction-ratio of the modulated signal is reduced from 16.8 to 3.9 dB at 15 GHz. The characterization of the amplitude modulation suppression is presented, with good results for broadband operation (15 nm at 1557 nm), high-speed (up to 35 GHz) and high input extinction-ratio (up to 16.8 dB). (C) 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 53: 1286-1290, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.2601053612861290Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FINEP (Financiadora de Estudos e Projetos)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Nonhomogeneous current injection for the enhancement of semiconductor optical amplifier-based wavelength converters

Experimental data and calibrated simulations for an enhanced wavelength converter based on semiconductor optical amplifiers cross-gain modulation are presented. Theoretical results for spectral components along the SOA cavity are discussed and a nonhomogeneous bias current injection profile is suggested with an improvement of 4 dB in the XGM wavelength converted eye-opening. (c) 2006 Wiley Periodicals, Inc.4861141114

Mechanical, physical, and chemical characterization of Ti-35Nb-5Zr and Ti-35Nb-10Zr casting alloys

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Titanium is used due its excellent properties in medical and dentistry areas. With the objective of exploiting better mechanical properties, not altering its biocompatibility, it was intended to add niobium and zirconium to the titanium, being formulated two alloys Ti-35%Nb-5%Zr (alloy 1) and Ti-35%Nb-10%Zr (alloy 2) wt% produced by an arc melting method. The chemical analysis of the samples was accomplished by X-ray fluorescence, and the microstrutural evaluation by scanning electron microscopy and X-ray diffraction. The mechanical tests were: Vickers hardness, tensile strength, mechanical cycling, and fracture analysis. The results allowed characterizing the alloy 1 as alpha + beta type and the alloy 2 as beta type. It is found that the alloy 1 presented larger hardness and smaller tensile strength than the alloy 2. The fractures, after the tensile test, were of the ductile type and, after the mechanical cycling, they were of the mixed type for both alloys.20816291636Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)This study was performed in order to develop a novel approach based on antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) samples. For that purpose Wistar rats immunosuppressed or not were experimentally infected with Strongyloides venezuelensis. The microtiter plates were coated with alkaline parasite extract for antibody detection and with IgG anti-S. venezuelensis for antigen and immune complex detection. The immune serum was able to detect 1.56 mu g/mL of L3 antigens in BALF samples. ELISA sensitivity was 96.6%, 71.6% and 91.6% for antigen, antibody and immune complex, respectively, and the specificity was 100% for all methods. Antigen detection in BALF samples showed to be a good approach for evaluating the kinetics of infection in non immunosuppressed or immunosuppressed rats. IgG was detected in non immunosuppressed rats from day 8 p.i. and in immunosuppressed rats from day 2 p.i. Moreover, immune complex was detected during the entire kinetic for both groups. In conclusion, association of antigen, antibody and immune complex detection in BALF samples seems to be an alternative approach for early strongyloidiasis diagnosis particularly in immunosuppressed individuals. (C) 2012 Elsevier B.V. All rights reserved.1242166169Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES