A pesquisa participante na formação didático-pedagógica de professores de enfermagem La investigación participativa em la formación didáctico-pedagógica de professores de enfermería Participant-research in the didatic-pedagogical formation of faculty nurses

Neste relato a Pesquisa Participante é o referencial metodológico impulsionador para o trabalho coletivo empreendido por um grupo de enfermeiras-professoras da disciplina de Fundamentos de Enfermagem da Universidade Estadual de Londrina. Das discussões provenientes do processo participativo, que foram alicerçadas no referencial teórico da Pedagogia Histórico-Crítica, selecionou-se as concepções e práticas pedagógicas mais relevantes, através da apresentação e discussão das categorias Objetivos, Conteúdos, Metodologia e Avaliação. Concluiu-se que as reflexões produzidas no e com o grupo participante sobre os aspectos pedagógicos repercutiram na concepção e no fazer das docentes envolvidas e que o processo coletivo deve ser contínuo para o aperfeiçoamento pedagógico destes profissionais, agentes educativos preocupados com a formação de futuros enfermeiros críticos e transformadores da realidade.<br>En este relato la Investigación Participativa es la referencia metodológica impulsora para el trabajo colectivo emprendido por un grupo de enfermeras-profesoras de la asignatura de Fundamentos de Enfermería de la Universidad Estadual de Londrina. De las discusiones provenientes del proceso participativo, que se basaron en la referencia teórica de la Pedagogía Histórico-Crítica, se seleccionaron los conceptos y prácticas pedagógicas más importantes, a través de la presentación y discusión de las categorías Objetivos, Contenidos, Metodología y Evaluación, se llegó a concluir que las reflexiones producidas, en y con el grupo participante sobre los aspectos pedagógicos, repercutieron en la concepción y en el quehacer de las docentes comprometidas y que el proceso colectivo debe ser continuo para poder capacitar pedagógicamente estas profesionales, los agentes educacionales preocupados con la formación de futuros enfermeros críticos y transformadores de la realidad.<br>Through the participant-research framework, this collective study was carried out by a group of faculty nurses responsible for the course of Nursing Fundamentals at Londrina State University. Considering the discussion that emerged from the participative process and based on the Historical-Critical Pedagogy, the most relevant concepts and pedagogic practices were selected, through the presentation and discussion of the following categories: Goals, Contents, Methodology and Evaluation. Authors concluded that the reflections emerged by and with the group about pedagogic aspects effected the performance of the involved faculty as well as that the collective process must be continuous in order to support the pedagogic development of these professionals, who are educational agents concerned with the improvement of nurses' formation

The Genome Sequence Of The Gram-positive Sugarcane Pathogen Leifsonia Xyli Subsp. Xyli

The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Although L. xyli subsp. xyli has only been identified colonizing the xylem vessels of sugarcane, the numbers of predicted regulatory genes and sugar transporters are similar to those in free-living organisms. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. 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Comparative Genomics Of Two Leptospira Interrogans Serovars Reveals Novel Insights Into Physiology And Pathogenesis

Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing of Leptospira interrogans serovar Copenhageni and comparative analysis with the available Leptospira interrogans serovar Lai genome reveal that despite overall genetic similarity there are significant structural differences, including a large chromosomal inversion and extensive variation in the number and distribution of insertion sequence elements. Genome sequence analysis elucidates many of the novel aspects of leptospiral physiology relating to energy metabolism, oxygen tolerance, two-component signal transduction systems, and mechanisms of pathogenesis. A broad array of transcriptional regulation proteins and two new families of afimbrial adhesins which contribute to host tissue colonization in the early steps of infection were identified. 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Genome features of Leptospira interrogans serovar Copenhageni

We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set of genes encoding the enzymes for the cobalamin biosynthetic pathway and the novel coding genes related to lipopolysaccharide biosynthesis should bring new light to the study of Leptospira physiology. Genes related to toxins, lipoproteins and several surface-exposed proteins may facilitate a better understanding of the Leptospira pathogenesis and may serve as potential candidates for vaccine