Genetic characterization of Varroa destructor (Family: Varroidae) prevalent in honeybees (Apis mellifera) in the province of Aydin in Turkey

The aim of the present study was to identify the haplotypes of the Varroa destructor mite which infects honeybees in the province of Aydın in Turkey, using two different modified techniques for the mitochondrial Cox1 gene of the mite. In order to confirm the haplotype, two primers differing in their sequence i.e. (ADA 01) as forward primer 5′-TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′- GCCCCTATTCTTAATACATAGTGAAAATG-3′ and (ADA 02) with COXF primer [5′GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3′] and COXRa primer [5′GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3′], were selected. Amplified DNA 376 bp in size was acquired using (ADA 01) forward primer 5′- TACAAAGAGGGAAGAAGCAGCC-3′ and reverse primer 5′- GCCCCTATTCTTAATACATAGTGAAAATG-3′. SacI restriction enzyme was applied to the amplified products; however, this restriction enzyme did not cut the DNA. Amplified DNA, 570 bp in size was obtained using (ADA 02) COXF primer [5’GG(A/G)GG(A/T)GA(C/T)CC(A/T)ATT(C/T)T(A/T)TATCAAC3’] COXRa and [5’GG(A/T)GACCTGT(A/TA(A/T)AATAGCAAATAC3’]. XhoI and SacI restriction enzymes were used for the amplified products. Although, the SacI restriction enzyme did not cut the DNA, the XhoI restriction enzyme cut the amplified DNA into two fragments (bands), with the sizes of 270 and 300 bp two bands 270 and 300 bp. While comparing the results, these bands were found specific for Korean haplotype of V. destructor. In conclusion, all of the 200 samples of V. destructor examined in this study were identified to be the Korean haplotype

Parasitological and Pathological Investigations on Linguatula serrata nymphs in Mesenteric Lymph Nodes in Sheep In Konya Region in Turkey

In this study was aimed to investigate spread and pathological findings of Linguatula serrata nymphs in mesenteric lymph nodes (MLNs) in sheep. A total of 1000 MLNs from 140 sheep (125 male and 15 female) were collected from a commercial (Konet) abattoir. Fourteen of 140 sheep (10%) were infested with nymph stages of L. serrata.Infested lymph nodes were swollen, firm, edematous, many yellowish-white pinpoint and with congestion. In histopathological examinations, these nodules were consist of one or more nymphal stages of L. serrata and these were surrounded by infiltration mononuclear cells and neutrophyl leucocyte. Lymphoid follicules were hyperplastic and sinus catarrh was also observed in medulla

Van Bölgesindeki Bal Arılarında (Apis mellifera) Bulunan Varroa destructor’un (AKAR: Varroidae) Genetik Karakterizasyonu

Türkiye’de arı yetiştiriciliğinin en önemli arı zararlısı olarak görülen Varroa destructor, bir ektoparazittir.  Çalışmanın amacı, Türkiye’nin Van bölgesindeki bal arılarını (Apis mellifera) istila eden Varroa destructor akarının haplotiplerini belirlemektir. Araştırmada, akarın mitokondriyal Cox1 gen belgesini analiz etmek için PCR+RFLP modifiye yöntemi kullanılmıştır. Ayrıca örneklerin % 10’u (28) çift yönlü sekans analizi için özel bir firmaya gönderilmiştir. PCR sonucunda, 570 bp büyüklüğünde amplifiye DNA elde edilmiştir. RFLP için amplifiye ürüne XhoI ve SacI restriksiyon enzimleri uygulanmıştır. SacI restriksiyon enzimi DNA’yı kesmemesine rağmen XhoI restriksiyon enzimi amplifiye DNA’yı 270 ve 300 bp olarak iki parçaya (bant) kesmiştir. RFLP reaksiyonunun bantları ve mitokondriyal DNA'nın dizi analizi, tüm akar örneklerinin V. destructor Kore haplotipi olduğunu ortaya koymuştur. Bu çalışma sonuçları Van bölgesinde bu akarların kontrolünde yardımcı olmalıdır