Study The Chemical Kinetics Properties for Adenosine Deaminase Enzyme (Aminohydrolase EC 3.5.4.4) ADA In Breast Cancer Patients

This study involved(50) women with newly diagnostic breast cancer and before any treatments, their ages ranges between (26-61) years old, the patients (women) subjected to different breast cancer investigations, at this study, we concerned on one of the biochemical tools that used as indicant to breast cancer disease which is Adenosine Deaminase Enzyme (ADA),and study their chemical kinetics properties and with factors that effect on enzyme activity which includes ,The optimal activity were in pH (7.2), as well as the optimal temperatures for activity were 35ºC. and the optimal substrate and enzyme concentration were (0.034Mm) and (0.25µg/ml) respectively, and the incubation time was 30 min. The results of kinetics characterization for ADA results demonstrated Km ,Vmax, Activation energy (Ea) and Temperature coefficient Q10 values were 0.849 mM and 83mMol/l/min, 3927.17cal/mol , 2 respectively

Inflammatory and bone biomarkers/composites as a predictive tool for clinical characteristics of rheumatoid arthritis patients

Rheumatoid arthritis (RA) is related to alterations in different inflammatory and connective tissue biomarkers. The diagnostic values and the factors affecting these biomarkers are conflicting. In the present study, a bone-related composite (B-composite), made from the z-score of stromelysin-1 (MMP3), colony-stimulating factor 2 (CSF2), and osteopontin (OPN), and I-composite, reflecting immune activation, made from the z-score of tumor necrosis factor-α (TNFα), interferon-γ (INFγ), and vascular endothelial growth factor-A (VEGF) were examined in RA patients. The biomarkers were measured by ELISA technique in 102 RA patients and 58 age-matched healthy control subjects. Serum MMP3, TNFα, IFNγ, and CSF2 showed significant elevation in RA patients. Multivariate general linear model (GLM) analysis revealed a significant high effect of diagnosis on biomarkers' level (partial η2 = 0.415). Duration of disease is significantly associated with VEGF, OPN, and B-composite and negatively correlated with TNFα. B-composite is significantly associated with CRP. A significant fraction of the DAS28 score variance can be explained by the regression on zlnINFγ. The variance in the CRP was explained by zlnOPN and B-composite. More than half of anti-citrullinated protein antibodies (ACPA) variation can be explained by the regression on serum MMP3 and I-composite. The top 3 sensitive predictors for RA disease are INFγ, MMP3, and TNFα. B-composite is associated with the duration of disease and CRP. At the same time, I-composite is negatively associated with the ACPA level. The biomarker composites have potential use as RA disease characteristic biomarkers

Effect of Magnetic Field on Peroxidase Activity and Growth of Panicum miliaceum L. Seeds

In latest years in agricultural sector, physical techniques based on magnetic field are being designed. The present work studied the impact of magnetic field on the germination of fresh and partially-aged Panicum miliaceum L. (PM) seeds. The germinated PM seeds were categorized into 6 groups. Groups 1,2 and 3 were fresh seeds, and Groups 4, 5 and 6 (LV30%) were left for 6 days to lose about 30% of their viabilityLV30% seeds. Groups 1 and 4 were normal unexposed seeds. Groups 2 and 5 were seeds exposed to magnetic field intensity of 100mT, while those of 3 and 6 to 200mT for 60 minutes. Germination was monitored on days 2, 5, 7, 10, 12, 14 and 16. The effect of magnetic field on the % germination, growth characteristics of radical and shoot, protein concentration and peroxidase activity in fresh and LV30%were studied. Both the exposed and unexposed seeds lost their viability by 16 days of ageing in fresh and 14 days in (LV30%). Exposed seeds to magnetic fields 100 and 200 mT revealed significant increase in the length of root and shoot compared to normal unexposed seeds. The magnetically exposed aged seeds had significantly higher protein than in the unexposed aged seeds. The peroxidase activity decreased with ageing and the magnetically exposed seeds showed higher activity than the corresponding aged unexposed normal seeds. The present study suggests the magnetic field could accelerate the germination of PM seeds, accelerate their growth characteristics and increase soluble protein content. In addition the peroxidase activity significantly improved

SYNTHESIS, CHARACTERIZATION AND KINETIC STUDIES OF SOME OXAZEPINE AND OXAZEPANE DERIVATIVES

1,3-Bis(2-hydroxy-benzylidene)-Thiourea and 1,3-Bis-(dimethylamino-benzylidene)-Thiourea were prepared by condensation of Thiourea with one equivalent and two equivalent of substituted benzaldehyde. These Schiff-bases were reacted with one equivalent of Maleic , Succinic and Phthalic anhydride in absolute ethanol to give 7-membered heterocyclic ring system of 2-(2-Hydroxy-phenyl)-4,7-dioxo-4,7-dihydro-[1,3] oxazpine-3-carbothioic acid amide and 2-(4-Dimethylamino-phenyl)-4,7-dioxo-4,7-dihydro-[1,3]oxazepine-3-carbothioiacid amide , 1,3-Bis(2-hydroxy-benzylidene)-Thiourea and 1,3-Bis-(dimethylamino-benzylidene)-Thiourea were reacted with two equivalent of Maleic and Succinic anhydride in same solvent to give 2 (7-membered) heterocyclic ring system of 2-(2-hydroxy-phenyl)-3-[2-(2-hydroxy-phenyl)-4,7-dioxo-[1,3]oxazepine-3-carbothioyl]-2,3-dihydro-[1,3]oxazepine-a,7-dione and 2-(4-Dimethylamino-phenyl)-3-[2-(4-dimethylamino-phenyl)-4,7-dioxo-[1,3]oxazepine-3- carbothioyl]-2,3-dihydro-[1,3]oxazepine-4,7-dione. The constants of reaction velocity for (Schiff-bases)with Maleic , Succinic and Phthalic anhydride were studied and showed that the reaction wsa first – order one .Some of thermodynamic characteristics were evaluated and showed differences among the prepared compounds . The final products were identified by their melting points, elemental analysis, IR, 1H NMR and UV-Visible spectra

International Consensus Statement on Rhinology and Allergy: Rhinosinusitis

Background: The 5 years since the publication of the first International Consensus Statement on Allergy and Rhinology: Rhinosinusitis (ICAR‐RS) has witnessed foundational progress in our understanding and treatment of rhinologic disease. These advances are reflected within the more than 40 new topics covered within the ICAR‐RS‐2021 as well as updates to the original 140 topics. This executive summary consolidates the evidence‐based findings of the document. Methods: ICAR‐RS presents over 180 topics in the forms of evidence‐based reviews with recommendations (EBRRs), evidence‐based reviews, and literature reviews. The highest grade structured recommendations of the EBRR sections are summarized in this executive summary. Results: ICAR‐RS‐2021 covers 22 topics regarding the medical management of RS, which are grade A/B and are presented in the executive summary. Additionally, 4 topics regarding the surgical management of RS are grade A/B and are presented in the executive summary. Finally, a comprehensive evidence‐based management algorithm is provided. Conclusion: This ICAR‐RS‐2021 executive summary provides a compilation of the evidence‐based recommendations for medical and surgical treatment of the most common forms of RS

Study the Oxidative Stress, Some Biochemical and Hematological Parameters in patients with agout at AL-Ramadi city

This study aims to study the effect of gout disease on complete blood picture and biochemical parameters and some non-enzymatic antioxidants, some tracing elements and lipid peroxidation ,in outpatients with gout disease at Al-Ramadi Teaching-Hospital ,Al-Razi Hospital and the study duration from Octo.2013-to May 2014.(50) blood samples were collected from patients with age groups (30-80 years) from both sexes (28 males,22 females),a (30) blood samples (15 males,15 females) were collected from normal individuals as a control group with age groups (27-75 years). Hematological measurement showed no significant differences in size compressed blood cells, the percentages in ( 45.15 +4.99 and 46.87+6.30) % in patient and control groups respectively, hemoglobin concentrations were ( 14.04+1.66 and 14.30+1.93) g/l in patient and control groups respectively, total number of red blood cells ( 5.21+0.43 and 5.12 +0.58) 106/mm3 in patient and control groups respectively with(P?0.05) in ESR (21.06+13.47 and 13.37 +7.45) mm/hr in patient and control groups respectively with (P?0.05), the total number of WBCs were recorded (8.96+2.04 and 7.50+1.69)in patient and control groups respectively. Results showed also significant differences (P?0.05) in uric acid levels (7.42+0.76 and 5.62+0.88) mg/dl,malondialdehyde levels were recorded (4.45+0.64 and 3.21+0.86) in patient and control group

The Effect of Anemia on the Total Levels of Protein and Cholesterol in during Delivery Women and Their New born.

The study includes two main groups; the first group consists of (90) during delivery women, while the second consists of (90) new born children. The two groups are divided into other subgroups depending on whether they are anemic and non anemic. The first group contains (66) anemic women and (24) non anemic women, While the second group contains (23) anemic newborn and (67) non anemic new born. Total levels of protein and cholesterol have been checked in the subgroups in addition to the effect of anemia on these variables and the changes in total cholesterol and protein levels in regard to weight of the newly born. Variations of these variables depending on sex in the subgroups of newly born have also been checked. The study has revealed that total levels of cholesterol in during delivery anemic women were significantly high in comparison with their levels in non anemic women, their rates range (186 ±18.51) and (163±23.54) mg/dl for each of the two groups, respectively. Moreover, total protein levels were higher in non anemic women than in anemic women ranging (6.97±0.76) and (6.16±0.6) gm/dl, respectively (p < 0.05). Total cholesterol levels in anemic newborn were not significantly different from non anemic children, whereas total protein levels in non anemic children were higher compared with anemic children , rates rang (7.59±0.72) and (6.69 ±0.91) respectively(p<0.05). As for the relationship between the sex of the child and the studied biochemical variables in the sub groups, child sex had no effect on total cholesterol levels, whereas total protein levels varied; Differences were significant between male and female anemic children, while differences were not significant between male and female non anemic children. Also differences were significant between anemic and non anemic male children, whereas they were significant between anemic and non anemic female children (p<0.05). The study has also shown that weights of non anemic children were higher in comparison with anemic children, they were (3.4±0.38) and (3.02±0.47) respectively (p<0.05). Differences in weight between female and male anemic children were not significant; also they were not significant in male and female non anemic children. Differences in weight between anemic and non anemic children were significant; also they were significant between anemic and non anemic female children. There was a difference in the relationship between weights of anemic and non anemic children and total levels of protein and cholesterol in female and male children. In non anemic children, the relationship between total protein levels and the weight of male and female children was slightly significant (r= 0.357), while it was not significant in terms of total cholesterol levels with weights of female and male children. There was a reversible relationship between total protein levels in anemic male children and their weights (r=-0.448), while in female children the relationship was slightly significant(r=0.313). Total cholesterol levels in anemic male children had a high significant relationship (r=0.914) with their weight, while it was not significant in anemic female children

Effect of thickness on optical properties of pure Epoxy Resin plates

In this study, Epoxy Resin plates was prepared by mixing epoxy(A) and hardner(B)with ratio(A:B) (3:1) with different thickness (0.3-0.96)cm. The effect of thickness on optical properties have been studied (absorption ,transmission ,reflectance) also the optical constant were found like (absorption coefficient, extenuation coefficient and refraction index) for all of the prepared plates. The results have shown that by increasing the thickness of plates., the absorption intensity increase in which at plates thickness (0.3-0.96)cm the absorption intensity were(1.54-1.43) respectively, and since absorption peak for epoxy occur in ultraviolet region and exactly at wavelength(368)nm and energy gap(Eg=3.05 eV) thus their good transmittance in the visible light region The plates have transmittance of about (60-83.4)% in visible region ,the refraction index for Elda epoxy is (n= 1.53 ) and its reflectance is (R=4 )% at wavelength (368 nm)

Determination of some antioxidant in the serum of pregnant women and its relationship with some biochemical parameters

The aim of this study determine of some biochemical parameters in serum of pregnant women. The possibility of using these parameters as an indicator of duration of pregnancy. This study was conducted on 75 of blood samples from healthy pregnant women , from 1/2 /2016 to 30/6/2016 divided into three groups. aged (17 – 43 ) years , and the study samples comparad with 25 sample for healthy women not pregnant as a control. A comparison in hematological parameters between the three groups with each other and with the control showed there was a significant difference in total count white blood cell between the three groups and the control (P≤0.05) and the highest level was in group III. Respect to Hb and PCV% t results showed low significant in Group III when compared with groups I and II. The result of study showed a non-significant (p≤0.05) in total protein of all studied groups and control group , albumin were significantly decreased in the groups II and III compared to non-pregnant women, but there was no significant difference between the three groups in globulin and glucose(RSG). The activity of catalase and uric acid concentration were significantly lower in pregnant women in the groups I,II and III as compared to control group

Complex Synthesis of Cis-[Pt(Asc) (NH₃)₂] and its Effect on Human Breast Cancer MCF-7 Cell <i>in vitro</i>

Bosom malignancy is the most regularly analyzed disease and the imperative reason for growth-related passing among ladies, accounting for 23% of all new tumor cases and 14% of tumor passing's. L-Ascorbic acid, commonly known as vitamin C is well-known in chemistry since long back. It has tremendous medical applications in several diseases. Therefore, in this paper five concentrations of complex cis-[Pt(Asc)(NH3)2] where Asc=L-ascorbic acid derivative on MCF-7 cell line to detect the changes in five cellular parameters (nuclear intensity, mitochondrial membrane potential, valid cell count, cytochrome C, and membrane permeability) after exposure with 24 h are investigated. The results showed that 400 μg/mL has the highest significant effect on the five parameters (nuclear intensity, mitochondrial membrane potential, valid cell count, cytochrome C, and membrane permeability) when compared with Doxorubicin 20 μM (substance used as anti-cancer) which represent the positive control. Also, the 200 μg/mL showed results close to those of the untreated cells which represent the negative control (-ve) with a very few significant differences