POLYMERIC MATERIALS IN GLUING TECHNIQUES

Polymeric materials have an important role in gluing technology due to their broad application in the wood industry, metal industry, glass, and ceramics industry as well as medicine. This article discusses the basic physicochemical aspects of bonding with adhesives and also, the mechanisms of action of the adhesive materials during bonding based on polymerization processes and different types of polymerization. It was emphasized that depending on the material used in gluing, various adhesives with different physicochemical characteristics facilitate and contribute to the quality of the bonded joints. In this way, it is an insured procedure that is suitable for materials that are sensitive to the effects of heat, because welding and soldering would deform the base material and often the total degradation of its mechanical properties

Content of Heavy Metals and Metalloids in Drinking Water in the Una-Sana Canton of the Federation of Bosnia and Herzegovina

Unsko-sanska županija bogata je prirodnom izvorskom vodom, kojom se opskrbljuje stanovništvo. Po prvi put je ispitana procjena kakvoće toksičnih i esencijalnih teških metala i metaloida u vodi za piće iz više izvorišta u osam općina i to: Bihaću, Cazinu, Bosanskoj Krupi, Bužimu, Velikoj Kladuši, Bosanskom Petrovcu, Sanskom Mostu i Ključu. U svibnju 2016. godine iz svake općine analizirano je po tri uzorka vode (ukupno 24). Mjerene su koncentracije arsena, olova, kadmija, kroma, nikla, kobalta, molibdena, fosfora, cinka i bakra. U pet uzoraka vode uočene su povećane koncentracije za Ni (do 20 µg dm–3) i Cr (do 50 µg dm–3) i smatraju se prirodnim podrijetlom. Međutim, u većem broju uzoraka (osam) koncentracije As su odstupale od dozvoljenih (do 10 µg dm–3), s maksimumom od 14,05 µg dm–3 i vjerojatno su od samog sastava zemljišta, ali i antropogenih izvora zbog postojanja odlagališta otpada u široj regiji izvorišta. Vrijednosti koncentracija ostalih elemenata Cd, Pb, Co, Cu, P, Mo i Zn bile su u granicama maksimalno dozvoljenih propisanih Pravilnicima u BiH, Direktivom o vodi za ljudsku potrošnju 98/83/EC, kao i smjernicama Svjetske zdravstvene organizacije (WHO). Sadržaj teških metala i metaloida u vodi za piće na području Unsko-sanske županije, osim za As ne predstavljaju opasnost za zdravlje ljudi. Ovo djelo je dano na korištenje pod licencom Creative Commons Imenovanje 4.0 međunarodna.The Una-Sana Canton is rich in natural spring water that supplies the population. Performed for the first time was a quality assessment of toxic and essential heavy metals and metalloids in drinking water from several sources in eight municipalities: Bihac, Cazin, Bosanska Krupa, Buzim, Velika Kladusa, Bosanski Petrovac, Sanski Most, and Kljuc. In May 2016, three samples were taken from each municipality (total 24). The concentrations of arsenic, lead, cadmium, chromium, nickel, cobalt, molybdenum, phosphorus, zinc, and copper were measured. In a small number of water samples, slightly increased concentrations of Ni (up to 20 µg dm–3) and Cr (up to 50 µg dm–3) were found, and considered to be of natural origin. However, in the majority of samples, the As concentrations differed from the allowed concentration (up to 10 µg dm–3), with a maximum of 14.05 µg dm–3, which probably originates from the very composition of the soil, but also from anthropogenic sources due to the existence of a landfill in the wider region of the source. The concentrations of other elements, Cd, Pb, Co, Cu, P, Mo, and Zn were within the maximum permissible limits prescribed by the Regulations of B&H, the EU Drinking Water Directive 98/83/EC, as well as the WHO guidelines. Except for As, the content of heavy metals and metalloids in the drinking water of Una-Sana Canton is not a danger to human health. This work is licensed under a Creative Commons Attribution 4.0 International License

Element contents and health risk assessment in wild edible mushrooms of Bosnia and Herzegovina

The content of macro- and microelements in dry samples of mushrooms of the species Macrolepiota procera, Boletus edulis and Cantharellus cibarius, collected at different areas in Bosnia and Herzegovina, was de- termined using the ICP-OES method (inductively coupled plasma optical emission spectrometry). Of the macro- elements, K is the most represented, followed by S, P, Mg, and the least represented Ca and Na. Zn is the most represented of the essential microelements, followed by Fe, Se, Cu, Mn and Co. Al is the most abundant of the other trace elements followed by Ni and Cr. Of the toxic metals, the most represented is Cd, followed by Pb and As. There are differences in the concentration of micro- and macroelements in the mushrooms analysed, depending on the area from which they were collected because natural geology and geochemistry influence the content of macro- and microelements in wild edible mushrooms. The results show that the analysed mushrooms can be considered a good source of essential elements. The study also assessed potential health risks of heavy metals and the target hazard quotient (THQ) for As, Cd, Pb, Cu, Zn, Ni and Cr in the analysed mushrooms was lower than the safe level. The carcinogenic risk index revealed that Cd and Ni are the most prevalent pollutants in the mushrooms studied

Synthesis, Microbiological Activity and In Silico Investigation for Some Synthesized Coumarin Derivatives

Four 4-hydroxycoumarin derivatives were synthesized and the structure was confirmed by NMR spectroscopy and Mass spectrometry. Tested compounds have shown significant antimicrobial activity against Bacillus subtilis subsp. spizizenii, Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis, and the effect of more halogens on the benzene nucleus, as well as the combination of halogen and alkyl groups, on the antimicrobial activity, was investigated. According to the docking study, these compounds can operate simultaneously on two enzymes, amylase and gyrase (1BAG and 1KZN), which are known to play an important role in bacterial life. Obtained docking study parameters for tested compounds showed an association with the in vitro results of the antimicrobial activity of these compounds. In silico tests of molecular properties of the tested compounds showed that the compounds met Lipinski\u27s rule of five. In this paper, the ADME parameters of tested compounds were also calculated: Caco2 (in vitro Caco2 cell permeability), HIA (human intestinal absorption), MDCK (in vitro Mandin Darby Canine Kidney (MDCK) cell permeability), TPSA (topological polar surface area), etc. This work is licensed under a Creative Commons Attribution 4.0 International License

Amino Acids Composition and Antioxidant Activity of Selected Mushrooms from Bosnia and Herzegovina

Many studies highlight the health benefits of mushrooms, which are consequently becoming more and more interesting for researchers. The content of amino acids (AA), total phenolic content (TPC), and antioxidative activity (AAc) were determined in wild as well as cultivated mushrooms. The AA included: L-tryptophan (Trp), L-arginine (Arg), L-cysteine (Cys), L-methionine (Met), L-alanine (Ala), L-phenylalanine (Phe), L-lysine (Lys), L-valine (Val), L-glycine (Gly), and L-leucine (Leu). The wild mushrooms: Lactarius piperatus, Amanita caesarea, Lactarius deliciosus, Lycoperdon pyriforme, Macrolepiota procera, and cultivated mushrooms: Agaricus bisporus, Boletus aestivalis, Cantharellus cibarius, Pleurotus ostreatus, and Agaricus bisporus var. avellaneus were investigated in this study. The AA was determined by HPTLC method and quantified with a Camag TLC scanner and WINCAT software by scanning the plates at 540 nm. The TPC was spectrophotometrically estimated as gallic acid equivalents/g of fresh weight according to Folin-Ciocalteu’s method. The radical scavenging activity (RSA) of mushroom extracts was determined by DPPH assay. The highest content of Ala, Gly, Phe, Lys, Val, and Leu was found in Pleurotus ostreatus. The total phenolic content (mg GAE/g) in investigated mushrooms ranged from 1.90 to 35.56, and the % RSA ranged from 43.88 to 90.17. This study promotes the consumption of food rich in bioactive compounds, mushrooms being among such food. Further research on mushrooms from Bosnia and Herzegovina and their benefits in the overall maintenance of human health and protection from age-related diseases is necessary

Antiproliferative and genotoxic potential of xanthen-3-one derivatives

Twelve previously synthesized, biologically active 2,6,7-trihydroxyxanthen-3-one derivatives were evaluated in vitro for antiproliferative activity. Compounds were screened against HeLa, SW620, HepG2 and A549 tumor cell lines. Compound with the trifluormethyl group on C-4\u27 position of the phenyl ring showed the best inhibitory activity towards HeLa and A549 tumor cells with IC50 of 0.7 µmol L–1 4.1 µmol L–1, resp. Compound with chlorine and fluorine substituents on aryl ring showed the best antiproliferative activity against SW620 with IC50 of 4.1 µmol L–1 and against HepG2 tumor cell line with IC50 of 4.2 µmol L–1. Analyses of cytotoxic and genotoxic potential of the trifluormethyl derivative were performed with cytokinesis-block micronucleus cytome assay in human lymphocyte culture and revealed no genotoxic and cytotoxic effects. The most potent compounds were subjected to molecular docking simulations in order to analyse bindings to molecular targets and, at the same time, further support the results of experimental cytotoxic tests. Docking studies showed sites of importance in forming hydrogen bonds of the most potent compounds with targets of interest

With food to health : proceedings of the 9th International scientific and professional conference

Proceedings contains 7 original scientific papers, 8 professional papers and 1 review paper which were presented at "9th International Scientific and Professional Conference WITH FOOD TO HEALTH", organised in following sections: Nutrition, Dietetics and diet therapy, Food safety, Food analysis, Production of safe food and food with added nutritional value

Total Content of Phenols and Anthocyanins in Edible Fruits from Bosnia

Content of total phenols and total anthocyanins was estimated in edible fruits from Bosnia by photometric methods. Cyanidin-3-galactoside chloride was used as a standard for determination of total anthocyanins, and galic acid served as a standard for determination of total phenols. Total content of phenols was 12.7 mg/g in elderberry fruits, 10.4 mg/g in bilberry, 9.8 mg/g in blackberry, 8.8 mg/g in wild cherry, 6.1 mg/g in cultivated blackberry, 3.5 mg/g in cultivated strawberry, 2.4 mg/g in average in sour cherry fruits from different locations and the lowest quantity of total phenols was in edible parts of melon, only 0.2 mg/g. Total content of anthocyanins was 6.8 mg/g in wild cherry, 6.7 mg/g in elderberry fruits and 4.5 mg/g in bilberry. Wild bilberry fruits from different locations had in average 3.5 mg/g, cherries from different locations 1.3 mg/g, cultivated blackberries 1.0 mg/g, cultivated strawberries 0.8 mg/ g while melon fruit had no anthocyanins at all. Acidity was measured in macerate of edible fruits by direct insertion of electrode. pH values in the macerates were as follows: 3.03 in bilberry, 3.45 in blackberries, 3.59 in sour cherries, 3.92 in wild cherries, 4.44 in elderberries and 6.19 in melon

Antioxidant Capacity in the Lipophilic Fraction of Alzheimer’s Brain Tissues

The aim of this study was to investigate the antioxidant capacity (AC) in the lipophilic fraction of postmortem motorcortex (MC), nucleus caudatus (NC) and gyrus temporalis (GT) from controls (C) and Alzheimer’s disease (AD) patients. The initial samples consisted of 50 human brain tissues of AD and C. AC of the different region of human brain were measured by using the fluorescent method of the oxygen radical absorbance capacity (ORAC). Peroxyl and hydroxyl radical generators were used in the analysis. All ORAC analysis were carried out on the Perkin-Elmer spectrofluorometer LS 55 with fluorescent filters, Ex: 485 nm; Em 520 nm. Final results were calculated using the differences between area under the quenching curve of fluorescein (FL), blank and analyzed biological samples. AC against peroxyl radicals (ORAC-ROO°) of lipophilic fraction in MC of AD was statistically significantly lower in comparison with MC of C (p < 0,008). No changes in the AC against hydroxyl radicals (ORAC-°OH) of lipophilic fraction of AD were found in comparison with C. Reduction of total protein in GT of AD (p < 0,03) was found. The results showed that in the MC of AD brain the balance between production of free radicals and the neutralization by a complex antioxidant system is disturbed. The manual fluorescent method for AC measurements proved to be sufficiently appropriate and sensitive for the AC measurements of lipophilic fraction of postmortem brain tissues from different patologica! conditions

Inhibition of Neutral Red Photolysis with Different Antioxidants

Neutral red is a dye the azine structure which has been used as an acido-base indicator and a dye in histochemistry. In 1960 Goldhaber introduced Neutral red into the medium of resorbing bone cultures to localize the osteoclast in the living cultures. Using time-lapse microcinematography in order to follow the osteoclasts, he reported excellent contrast could be obtained with Neutral red due to the avidity of osteoclasts for this dye. Unfortunately, however, the photodynamic effect resulting from subsequent exposure of these cultures to light precluded this approach, and again in 1963. it was observed that the death of the osteoclasts was probably due to a photodynamic effect related to the dye in the cell, the presence of oxygen and the frequent exposure of light by our time-lapse photography. VIS and UV irradiation induced photolysis of Neutral red, and from Neutral red cation produced with photons a Neutral red radical. This Neutral red radical can be inhibited with action of an antioxidant, such as melatonin, glutathione, ascorbic acid, E vitamin, etc. We developed an assay with Neutral redphotolysis which utilizes a VIS and UV irradiation technique for quantification the inhibition of photolysis with action of an antioxidant. In this method Neutral red acts double, as a free radical generator and as a photosensitizer