83 research outputs found
Cytologic-enzymologic diagnosis of experimental pneumonia induced by klebsiella pneumoniae serotype ii in rats and its treatment with free and liposomal enrofloxacin
Density functional theory studies of MTSL nitroxide side chain conformations attached to an activation loop
A quantum-mechanical (QM) method rooted on density functional theory (DFT) linked to the Stochastic Liouville equation (SLE) in the Fokker Planck (FP) form has been employed for the first time to sample the methane-thiosulfonate spin label (MTSL) conformational space attached to the Aurora-A kinase activation loop and to calculate the EPR spectrum. The features of the calculated energy surface allowed us to describe the system in a limited number of rotamers stabilized by interactions of the MTSL side chain and neighbouring residues. The relevant magnetic parameters and the electron paramagnetic resonance (EPR) spectrum were subsequently calculated from the trajectories of the spin probe in the protein environment. The comparison between theoretical and experimental continuous wave (CW) EPR spectra revealed some small differences in the EPR line shape which arises from the combinations of g- and A-values obtained from the conformations selected. The theoretical approach adopted in this work can be used to recognise the contribution of MTSL rotamers to the EPR spectrum in order to help extract structural/dynamics properties of protein from the experimental data
New-onset diabetes after transplantation in tacrolimus-treated, living kidney transplantation: long-term impact and utility of the pre-transplant OGTT
Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label
Structural investigations of proteins and their biological complexes are now frequently complemented by distance constraints between spin labeled cysteines generated using double electronâelectron resonance (DEER) spectroscopy, via site directed spin labeling (SDSL). Methanethiosulfonate spin label (MTSSL), has become ubiquitous in the SDSL of proteins, however, has limitations owing to its high number of rotamers, and reducibility. In this article we introduce the use of bromoacrylaldehyde spin label (BASL) as a cysteine spin label, demonstrating an advantage over MTSSL due to its increased selectivity for surface cysteines, eliminating the need to âknock outâ superfluous cysteine residues. Applied to the multidomain protein, His domain protein tyrosine phosphatase (HD-PTP), we show that BASL can be easily added in excess with selective labeling, whereas MTSSL causes protein precipitation. Furthermore, using DEER, we were able to measure a single cysteine pair distance in a three cysteine domain within HD-PTP. The label has a further advantage of comprising a sulfide in a three-bond tether, making it a candidate for protein binding and in-cell studies
Migrant breast cancer patients and their participation in genetic counseling: results from a registry-based study
Evaluation of chitosan gel with 1% silver sulfadiazine as an alternative for burn wound treatment in rats
Integrated analysis of the conformation of a protein-linked spin label by crystallography, EPR and NMR spectroscopy
Therapeutic importance of sulfated polysaccharides from seaweeds: updating the recent findings
A Remark on Unified Error Exponents: Hypothesis Testing, Data Compression and Measure Concentration
Let A be finite set equipped with a probability distribution P, and let M be a âmassâ function on A. A characterization is given for the most efficient way in which A n can be covered using spheres of a fixed radius. A covering is a subset C n of A n with the property that most of the elements of A n are within some fixed distance from at least one element of C n , and âmost of the elementsâ means a set whose probability is exponentially close to one (with respect to the product distribution P n ). An efficient covering is one with small mass M n (C n ). With different choices for the geometry on A, this characterization gives various corollaries as special cases, including Martonâs error-exponents theorem in lossy data compression, Hoeffdingâs optimal hypothesis testing exponents, and a new sharp converse to some measure concentration inequalities on discrete spaces
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