2 nd Brazilian Consensus on Chagas Disease, 2015

Abstract Chagas disease is a neglected chronic condition with a high burden of morbidity and mortality. It has considerable psychological, social, and economic impacts. The disease represents a significant public health issue in Brazil, with different regional patterns. This document presents the evidence that resulted in the Brazilian Consensus on Chagas Disease. The objective was to review and standardize strategies for diagnosis, treatment, prevention, and control of Chagas disease in the country, based on the available scientific evidence. The consensus is based on the articulation and strategic contribution of renowned Brazilian experts with knowledge and experience on various aspects of the disease. It is the result of a close collaboration between the Brazilian Society of Tropical Medicine and the Ministry of Health. It is hoped that this document will strengthen the development of integrated actions against Chagas disease in the country, focusing on epidemiology, management, comprehensive care (including families and communities), communication, information, education, and research

Development and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnh

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Eucalyptus camaldulensis Dehnh is an Australian tree species which occurs in various climatic and environmental conditions and show large genetic diversity. Twenty five microsatellite markers were developed from a CT(8)-GT(8) enriched genomic library of E. camaldulensis. The number of alleles ranged from 4 to 13 (average of 8). The polymorphism information content (PIC) and the discriminating power (D) of each primer ranged from 0.37 to 0.88 (average of 0.72) and 0.48 to 0.99 (average of 0.84), respectively. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.25 to 0.90, respectively. Four loci showed statistically significant from Hardy-Weinberg equilibrium after Bonferroni correction (P, (5%) < 0.0038). All polymorphic markers were tested for cross-amplification in 25 different Eucalyptus species. Those microsatellite loci will be useful to investigate questions of genetic diversity and structure, gene flow, mating system and ex situ genetic conservation of E. camaldulensis.11465469Sao Paulo State GovernmentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP [05/58721-9, 05/59217-2

Microsatellite markers isolated from polyploid Kielmeyera coriacea Mart. & Zucc. (Clusiaceae) from an enriched genomic library

Kielmeyera coriacea is a morphologically highly variable polyploid species, characteristic of the Cerrado, the savanna-like vegetation of the Central-Brazil. It was subject of various biological studies and, like others cerrado plants, this species suffers strong anthropogenic pressure. To access the genetic diversity of populations of this species, its taxonomic circumscriptions and to study supposed hybridization events, we have developed 11 polymorphic microsatellite loci from a CT/GT-enriched genomic library. The band pattern showed 12-32 bands per locus, and 2-8 bands per locus per individual. All microsatellites successfully amplified PCR products in K. grandiflora, the putative sister species, and all revealed similar multibanded pattern.10515331535Coordenacao de Aperfeic, oamento de Pessoal de Nivel SuperiorFundo de Apoio ao Ensino, a Pesquisa e a Extensao da Universidade Estadual de Campinas [108807]Fundo de Apoio ao Ensino, a Pesquisa e a Extensao da Universidade Estadual de Campinas [108807

Isolation and characterization of microsatellite markers for Brachiaria brizantha (Hochst. ex A. Rich.) Stap

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The first set of nuclear simple sequence repeat (SSR) loci for Brachiaria brizantha (Hochst. ex A. Rich.) Stap is described. A microsatellite-enriched library was constructed and 19 loci were characterized. About 13 SSR loci were found to be polymorphic and across-taxa amplification tests showed that six of them can be transferred to four other species of Brachiaria. This new SSR resource will be a powerful tool for population genetic studies of B. brizantha, for interspecific genetic studies within the genus Brachiaria, for mapping and for marker assisted selection in breeding.10618731876Brazilian Agricultural Research Corporation (Embrapa)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Foundation for Science and Technological Development of the State of Mato Grosso do Sul (Fundect)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X-9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.11475479Brazilian Agricultural Research Corporation (EMBRAPA)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Foundation for Science and Technological Development of the State of Mato Grosso do Sul (FUNDECT)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Genetic diversity and population structure analysis of the tropical pasture grass Brachiaria humidicola based on microsatellites, cytogenetics, morphological traits, and geographical origin

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brachiaria humidicola (Rendle) Schweick. is a warm-season grass commonly used as forage in the tropics. Accessions of this species were collected in eastern Africa and massively introduced into South America in the 1980s. Several of these accessions form a germplasm collection at the Brazilian Agricultural Research Corporation. However, apomixis, ploidy, and limited knowledge of the genetic basis of this germplasm collection have constrained breeding activities. The objectives of this work were to identify genetic variability in the Brazilian B. humidicola germplasm collection using microsatellite markers and to compare the results with information on the following: (1) collection sites of the accessions; (2) reproductive mode and ploidy levels; and (3) genetic diversity revealed by morphological traits. The evaluated germplasm population is highly structured into four major groups. The sole sexual accession did not group with any of the clusters. Genetic dissimilarities did not correlate with either geographic distances or genetic distances inferred from morphological descriptors. Additionally, the genetic structure identified in this collection did not correspond to differences in ploidy level. Alleles exclusive to either sexual or apomictic accessions were identified, suggesting that further evaluation of the association of these loci with apospory should be carried out.539698709Brazilian Agricultural Research Corporation (EMBRAPA) [02.05.2.10.00.02.01, 01.06.1.07.05.06.02]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Foundation for Science and Technological Development of the State of Mato Grosso do Sul (FUNDECT) [41/100165/2004]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Agricultural Research Corporation (EMBRAPA) [02.05.2.10.00.02.01, 01.06.1.07.05.06.02]CNPq [482458/2007-0, 478262/2004-3, 307430/2007-3]FAPESP [2010/51010-0, 2007/57022-5, 2006/52953-8]Foundation for Science and Technological Development of the State of Mato Grosso do Sul (FUNDECT) [41/100165/2004