11 research outputs found

    Metabolic engineering of Rhizopus oryzae for the production of platform chemicals

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    Rhizopus oryzae is a filamentous fungus belonging to the Zygomycetes. It is among others known for its ability to produce the sustainable platform chemicals l-(+)-lactic acid, fumaric acid, and ethanol. During glycolysis, all fermentable carbon sources are metabolized to pyruvate and subsequently distributed over the pathways leading to the formation of these products. These platform chemicals are produced in high yields on a wide range of carbon sources. The yields are in excess of 85 % of the theoretical yield for l-(+)-lactic acid and ethanol and over 65 % for fumaric acid. The study and optimization of the metabolic pathways involved in the production of these compounds requires well-developed metabolic engineering tools and knowledge of the genetic makeup of this organism. This review focuses on the current metabolic engineering techniques available for R. oryzae and their application on the metabolic pathways of the main fermentation products

    Effect of Cultural Conditions on the Commercial Production of Polygalacturonase by Rhizoctonia bataticola

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    Polygalacturonase (PG) activity was detected in culture filtrate and Rhizoctonia-infected tissues of potato tubers (Solanum tuberosum). Culture produced PG formed more reducing groups that PG from diseased tissue. PG production and pH of the culture medium increased with incubation period and attained optimal values between the 4th and 6th day. The ability of Rhizoctonia bataticola to produce PG varied with the carbon and nitrogen sources in the medium; relatively large amounts of PG were produced in media supplemented with citrus pectin, sodium polypectate and potato slices as carbon sources and asparagine and D-L-_-phenylalanine as nitrogen sources. The industrial use of the enzyme to separate cellulose fibres and the maceration of vegetables are discussed. Keywords:Rhizoctonia bataticola, Solanum tuberosum, polygalacturonase, nitrogen and carbon sources. Discovery and Innovation Vol. 19 (4) 2007: pp. 264-26
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