Vaccination with newcastle disease vaccines strain i2 and lasota in commercial and local chickens in Plateau State Nigeria

Vaccination trials and comparative immunogenicity study using Newcastle disease vaccine strain I2 (NDVI2) and NDV La Sota administered to commercial and local chickens through intraocular (i/o), intramuscular (i/m), drinking water (dw), untreated sorghum, parboiled sorghum, sorghum coated with gum Arabic or commercial chick mash feed as vaccine carriers was conducted. Newcastle disease vaccine strain I2 and NDV La Sota vaccines provided protection to commercial and local chickens vaccinated through i/o, i/m or dw. No significant difference (P.0.05) was observed in the antibody titre of commercial or local chickens vaccinated with either NDVI2 or NDV La Sota vaccines administered via commercial feed, parboiled sorghum, parboiled sorghum coated with gum Arabic and untreated sorghum. NDVI2 or NDV La Sota vaccines administered through commercial feed, parboiled sorghum, parboiled sorghum coated with gum Arabic and untreated sorghum gave no or limited protection (0-22%) to the birds when challenged with a local strain of velogenic viscerotropic Newcastle disease virus Kudu 113 strain. It was concluded that the vaccine carriers used in this study were not suitable for delivery of NDVI2 or NDVS La Sota vaccines to local or commercial chickens

Concentration and Human Health Risk Assessment of Dichlorodiphenyltrichloroethane in Two Species of Fish Muscle from River Gongola Basin and its Dam, Dadinkowa, Gombe State, Nigeria

The concentration of DDT and its metabolites in the two fish species (Clarias gariepinus and Bagrus docmak) from River Gongola basin and its dam, Dadinkowa in Gombe State Nigeria was determined and human health risk association from the consumption of contaminated fish was evaluated. Fish samples were collected for period of six months and the pesticide residues analysis was carried out by using QuEChERS method along with conventional method (Hand shaken technique) and gas chromatography with electron capture detector (GC-ECD). (DDTs) and its metabolites were observed with DDD were the predominant contaminants, followed by DDT. The predominance of DDE may be attributed to their current use in vector control and contamination from past usage. The estimated daily intakes (EDIs) of DDT and its metabolites from all fish species were much lower than the acceptable daily intakes (ADIs), indicating that consumption of fish is at little risk to human health at present. However, the cancer risk estimates in the area of concern and the hazard ratios (HRs) of DDTs below the threshold value of one, indicating daily exposure to these compounds is a potential concern

Fungal Contamination of Some Poultry Houses in Kaduna State, Nigeria

The study was conducted to assess the level of fungal contamination of poultry houses with emphasis on Aspergillus species contamination of litter, feeders, drinkers and the housing materials. Ten swabs each from ten locations and materials (100) of the 10 poultry houses were collected for fungal isolation. A total of 126 fungi belonging to 5 genera were isolated from the different parts of the poultry environment viz; Aspergillus, Mucor, Candida, Rhizopusand Penecillium species with Aspergillus and Candida species having the highest frequency of isolation, 69 (54.76%) and 27 (20.93%) respectively. Three species of Aspergillus were isolated A. fumigatus (22), A. flavus (22) and A. niger (18). Fungi were isolated from all parts of the poultry sampled with a higher rate of isolation from the doors, window nets, roof and feeders. The presence of Aspergillus and Candida species which are important poultry pathogens i.e causing Aspergillosis, mycotoxicosis and Candidaisis indicates an economic threat the farmers as well as to the health of the bird

Malaria vectors resistance to commonly used insecticides in the control of malaria in Bichi, northern Nigeria

This study was carried out in 2015 to assess the level of resistance of sibling species of Anopheles gambiae complex the principal malaria vector from Bichi in Kano state to three classes of insecticides; (DDT, Permethrin and Bendiocarb) approved by World Health Organization (WHO) for vector control with the aim of obtaining baseline susceptibility and resistance data by evaluating the potency of brands of insecticides used against the mosquito vector in the area where the disease is prevalent. An. gambiae larvae collected from ecologically contrasting breeding sites were reared to adults in the laboratory. Adults from the F1 progeny were assayed for resistance using the WHO adult insecticide susceptibility bioassay protocol. The mosquitoes were exposed to DDT, permethrin and bendiocarb belonging to 3 classes of insecticides. The individual sibling species were identified molecularly and the molecular resistance mechanisms characterized by SINE and kdr PCR methods respectively. The An. gambiae populations tested revealed high level of resistance to DDT and permethrin but less resistant to bendiocarb. Analysis of the genetic composition of the vector population revealed predominance of An. coluzzii (> 77%). L1014F and L1014S kdr mutations correlated to insecticide resistance phenotype expression. This study indicates differential distribution of the resistant genotype of Anopheles malaria vector between ecologically different habitats in the area. This insecticide resistance represents a threat to vector control tool and should be monitored. The information obtained could be useful in decision and planning making for vector control programs in the region.Keywords: Anopheles coluzzii, Anopheles gambiae, Insecticides, Malaria control, resistance, Susceptibilit

Establishing convergent validity of a medication literacy assessment instrument for use within the Nigerian setting

Purpose: To establish convergent validity of a previously designed medication literacy instrument for use in Nigeria. Methods: A cross-sectional study was conducted in Zaria, Kaduna State from May to August 2021, with structured instruments administered to conveniently sampled members of the public via one-on-one interviews to collect data. These instruments included a previously designed medication literacy assessment instrument and the Newest Vital Sign United Kingdom version (NVS-UK) health literacy assessment questionnaire. Data obtained was reported using descriptive and inferential statistics. Results: Three hundred respondents were interviewed, majority of whom were females (51 %) and aged between 15 – 25 (76.6 %). The percentage of correct responses to the NVS-UK questions ranged from 22 to 58.3 %, while the total number of NVS-UK questions answered correctly by respondents ranged from 0 to 6 with a mean of 2.2 ± 1.7. Respondents’ NVS-UK scores were associated with their highest level of education completed (p = 0.001). The NVS-UK showed adequate internal consistency (Cronbach’s Alpha = 0.7) and validation of the developed medication literacy instrument against the NVS-UK demonstrated a Spearman’s rho correlation coefficient of 0.42. Conclusion: The designed instrument is valid and can be used to assess medication literacy within the country

Mitigating the Event and Effect of Energy Holes in Multi-hop Wireless Sensor Networks Using an Ultra-Low Power Wake-up Receiver and an Energy Scheduling Technique

This research work presents an algorithm for extending network lifetime in multi-hop wireless sensor networks (WSN). WSNs face energy gap issues around sink nodes due to the transmission of large amounts of data through nearby sensor nodes. The limited power supply to the nodes limits the lifetime of the network, which makes energy efficiency crucial. Multi-hop communication has been proposed as an efficient strategy, but its power consumption remains a research challenge. In this study, an algorithm is developed to mitigate energy holes around the sink nodes by using a modified ultra-low-power wake-up receiver and an energy scheduling technique. Efficient power scheduling reduces the power consumption of the relay node, and when the residual power of the sensor node falls below a defined threshold, the power emitters charge the nodes to eliminate energy-hole problems. The modified wake-up receiver improves sensor sensitivity while staying within the micro-power budget. This study's simulations showed that the developed RF energy harvesting algorithm outperformed previous work, achieving a 30% improvement in average charged energy (AEC), a 0.41% improvement in average energy (AEH), an 8.39% improvement in the number of energy transmitters, an 8.59% improvement in throughput, and a 0.19 decrease in outage probability compared to the existing network lifetime enhancement of multi-hop wireless sensor networks by RF Energy Harvesting algorithm. Overall, the enhanced power efficiency technique significantly improves the performance of WSNs

Localization of the Drosophila Rad9 Protein to the Nuclear Membrane Is Regulated by the C-Terminal Region and Is Affected in the Meiotic Checkpoint

Rad9, Rad1, and Hus1 (9-1-1) are part of the DNA integrity checkpoint control system. It was shown previously that the C-terminal end of the human Rad9 protein, which contains a nuclear localization sequence (NLS) nearby, is critical for the nuclear transport of Rad1 and Hus1. In this study, we show that in Drosophila, Hus1 is found in the cytoplasm, Rad1 is found throughout the entire cell and that Rad9 (DmRad9) is a nuclear protein. More specifically, DmRad9 exists in two alternatively spliced forms, DmRad9A and DmRad9B, where DmRad9B is localized at the cell nucleus, and DmRad9A is found on the nuclear membrane both in Drosophila tissues and also when expressed in mammalian cells. Whereas both alternatively spliced forms of DmRad9 contain a common NLS near the C terminus, the 32 C-terminal residues of DmRad9A, specific to this alternative splice form, are required for targeting the protein to the nuclear membrane. We further show that activation of a meiotic checkpoint by a DNA repair gene defect but not defects in the anchoring of meiotic chromosomes to the oocyte nuclear envelope upon ectopic expression of non-phosphorylatable Barrier to Autointegration Factor (BAF) dramatically affects DmRad9A localization. Thus, by studying the localization pattern of DmRad9, our study reveals that the DmRad9A C-terminal region targets the protein to the nuclear membrane, where it might play a role in response to the activation of the meiotic checkpoint

Super resolution of car plate images using generative adversarial networks

Car plate recognition is used in traffic monitoring and control systems such as intelligent parking lot management, finding stolen vehicles, and automated highway toll. In practice, Low-Resolution (LR) images or videos are widely used in surveillance systems. In low resolution surveillance systems, the car plate text is often illegible. Super-Resolution (SR) techniques can be used to improve the car plate quality by processing a series of LR images into a single High-Resolution (HR) image. Recovering the HR image from a single LR is still an ill-conditioned problem for SR. Previous methods always minimize the mean square loss in order to improve the peak signal to noise ratio (PSNR). However, minimizing the mean square loss leads to overly smoothed reconstructed image. In this paper, Generative Adversarial Networks (GANs) based SR is proposed to reconstruct the LR images into HR images. Besides that, perceptual loss is proposed to solve the smoothing issue. The quality of the GAN based SR generated images is compared to existing techniques such as bicubic, nearest and Super-Resolution Convolution Neural Network (SRCNN). The results show that the reconstructed images using GANs based SR achieve better results in term of perceptual quality compared to previous methods

Rocket borne instrumentation for the measurement of electron density-height profile in the ionosphere

The present paper describes the construction and working of a high frequency capacitance rocket payload for the measurement of electron concentration in the ionosphere, designed and fabricated mostly with indigenous components. This payload was flight-tested in the first and second Indian Centaure test rocket launched on 26-2-1969 and 7-12-1969 from Thumba Equatorial Rocket Launching Station. The paper also presents the calibration procedures and some of the preliminary results obtained from the first flight to show the capabilities of the instrument

Prevalence and Determinants of Endothelial Dysfunction among Adults Living with HIV in Northwest Nigeria

Background: Endothelial dysfunction constitutes an early pathophysiological event in atherogenesis and cardiovascular disease. This study aimed to assess the prevalence, determinants, and degree of endothelial dysfunction in antiretroviral therapy (ART)–treated people living with HIV (PLWH) in northwestern Nigeria using brachial flow-mediated dilatation (FMD). Methods: This was a comparative, cross-sectional study. A total of 200 ART-treated adults living with HIV with no evidence of kidney disease were compared with 200 HIV-negative participants attending a tertiary hospital in Kano, Nigeria, between September 2020 and May 2021. Endothelial function was evaluated by measuring FMD with a high-resolution vascular ultrasound transducer. FMD was calculated as the ratio of the brachial artery diameter after reactive hyperemia to baseline diameter and expressed as a percentage of change. Blood and urine samples were obtained from participants in both arms. Urine albumin-to-creatinine ratio (uACR) was calculated using the 2021 CKD-EPI estimated glomerular filtration rate (eGFR) creatinine-cystatin C equation without the race variable, and low-density lipoprotein (LDL) cholesterol was measured using enzymatic method. Results: The overall mean age (± standard deviation) of the study participants was 42 ± 11 years. Participants in the comparison arm were younger than PLWH (38 ± 11 versus 46 ± 10 years, respectively). The median (interquartile range) uACR was 41.6 (23.2–162.9) mg/g for the ART-treated PLWH versus 14.5 (7.4–27.0) mg/g for healthy controls. PLWH had a significantly lower mean percent FMD when compared to HIV-negative participants (9.8% ± 5.4 versus 12.1% ± 9.2, respectively). Reduced FMD was independently associated with HIV infection (β = –2.83%, 95% CI, –4.44% to –1.21%, p = 0.001), estimated glomerular filtration rate (β = –0.04%, 95% CI, –0.07% to –0.01%, p = 0.004) and LDL cholesterol (β = –1.12%, 95% CI, –2.13% to –0.11%, p = 0.029). Conclusion: HIV-positive status, lower estimated GFR, and higher LDL cholesterol levels were independently associated with endothelial dysfunction. Future prospective studies with larger cohorts of persons living with HIV (and age- and sex-matched HIV-negative controls) are needed to gain further insight into these important findings. In the interim, aggressive management of modifiable risk factors is warranted