Sacha Inchi (plukenetia Volubilis L.) Oil Composition Varies With Changes In Temperature And Pressure In Subcritical Extraction With N-propane

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sacha inchi is an oilseed crop that shows high oil content and it is an excellent source of polyunsaturated fatty acids. Temperature and pressure can influence in lipid composition on the subcritical extraction with n-propane. The highest extraction yield obtained was 30% under the conditions of 60 degrees C and 12 MPa. The extracted oil presented 442 mg of fatty acid g(-1) in terms of alpha linolenic acid. Analysis by easy ambient sonic-spray ionization mass spectrometry showed a typical TAG (Triacylglycerols) profile for Sacha inchi oil with major ions of m/z (mass-to-charge ratio) 895 (ALA-ALA-ALA), 897 (ALA-ALA-LA), 899 (ALA-LA-LA) and 901 (LA-LA-LA or OL-LA-ALA) in a form of adduct of [TAG + Na](+), in which ALA is alfa linoleic acid, LA is linoleic acid and OL is oleic acid. The phytosterols content (86.39-101.92 mg 100g(-1) of total lipids) varied according to the conditions applied for the n-propane extraction. In general, the subcritical fluid extraction with n-propane was found to preserve the important Sacha inchi oil bioactive constituents, that is, fatty acids, triacylglycerols and phytosterols. Regression analysis also showed that higher temperatures and pressures result in higher yields of phytosterols. (C) 2016 Elsevier B.V. All rights reserved.876470CapesSao Paulo Research Foundation (FAPESP) [2013/19161-4]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP