A devil of a transmissible cancer

Devil facial tumor disease (DFTD) encompasses two independent transmissible cancers that have killed the majority of Tasmanian devils. The cancer cells are derived from Schwann cells and are spread between devils during biting, a common behavior during the mating season. The Centers for Disease Control and Prevention (CDC) defines a parasite as "An organism that lives on or in a host organism and gets its food from, or at, the expense of its host." Most cancers, including DFTD, live within a host organism and derive resources from its host, and consequently have parasitic-like features. Devil facial tumor disease is a transmissible cancer and, therefore, DFTD shares one additional feature common to most parasites. Through direct contact between devils, DFTD has spread throughout the devil population. However, unlike many parasites, the DFTD cancer cells have a simple lifecycle and do not have either independent, vector-borne, or quiescent phases. To facilitate a description of devil facial tumor disease, this review uses life cycles of parasites as an analogy

Indigenous knowledge management - multimedia capture of oral history (New lamps for old)

The history or origins of a village has always been transmitted through oral storytelling by the older generations who have lived in that village for a long time. In order to collect and preserve this tacit knowledge, Perbadanan Perpustakaan Awam Selangor (PPAS) the Selangor State Public Library, in collaboration with Discovery Research Consultant Management Sdn. Bhd and Unit Perancang Ekonomi Negeri Selangor (The Selangor Stae Ecomonic Unit) decided to collect oral histories of villages in Sabak Bernam, which is one of the districts in Selangor, a state in Peninsular Malaysia. With the realization that knowledge is a core resource, they captured and documented all the oral histories (tacit knowledge) into a book (explicit knowledge) called “KOLEKSI CERITA RAKYAT: DAERAH SABAK BERNAM”. Through the oral histories told to them by the older inhabitants, they managed to collect 53 stories of villages in Sabak Bernam. Though the PPAS initiatives were good, the book failed to catch the younger generation’s attention. Now in conjunction with the Faculty of Creative Multimedia, Multimedia University, they have taken one step further by converting the book into animation format

Adaptive matched field processing in an uncertain propagation environment

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution January 1992Adaptive array processing algorithms have achieved widespread use because they are very effective at rejecting unwanted signals (i.e., controlling sidelobe levels) and in general have very good resolution (i.e., have narrow mainlobes). However, many adaptive high-resolution array processing algorithms suffer a significant degradation in performance in the presence of environmental mismatch. This sensitivity to environmental mismatch is of particular concern in problems such as long-range acoustic array processing in the ocean where the array processor's knowledge of the propagation characteristics of the ocean is imperfect. An Adaptive Minmax Matched Field Processor has been developed which combines adaptive matched field processing and minmax approximation techniques to achieve the effective interference rejection characteristic of adaptive processors while limiting the sensitivity of the processor to environmental mismatch. The derivation of the algorithm is carried out within the framework of minmax signal processing. The optimal array weights are those which minimize the maximum conditional mean squared estimation error at the output of a linear weight-and-sum beamformer. The error is conditioned on the propagation characteristics of the environment and the maximum is evaluated over the range of environmental conditions in which the processor is expected to operate. The theorems developed using this framework characterize the solutions to the minmax array weight problem, and relate the optimal minmax array weights to the solution to a particular type of Wiener filtering problem. This relationship makes possible the development of an efficient algorithm for calculating the optimal minmax array weights and the associated estimate of the signal power emitted by a source at the array focal point. An important feature of this algorithm is that it is guarenteed to converge to an exact solution for the array weights and estimated signal power in a finite number of iterations. The Adaptive Minmax Matched Field Processor can also be interpreted as a two-stage Minimum Variance Distortionless Response (MVDR) Matched Field Processor. The first stage of this processor generates an estimate of the replica vector of the signal emitted by a source at the array focal point, and the second stage is a traditional MVDR Matched Field Processor implemented using the estimate of the signal replica vector. Computer simulations using several environmental models and types of environmental uncertainty have shown that the resolution and interference rejection capability of the Adaptive Minmax Matched Field Processor is close to that of a traditional MVDR Matched Field Processor which has perfect knowledge of the characteristics of the propagation environment and far exceeds that of the Bartlett Matched Field Processor. In addition, the simulations show that the Adaptive Minmax Matched Field Processor is able to maintain it's accuracy, resolution and interference rejection capability when it's knowledge of the environment is only approximate, and is therefore much less sensitive to environmental mismatch than is the traditional MVDR Matched Field Processor.The National Science Foundation, the General Electric Foundation, the Office of Naval Research, the Defense Advanced Research Projects Agency, and the Woods Hole Oceanographic Institution

Molecular Epidemiology of Staphylococcus aureus Skin and Soft Tissue Infections in the Lao People's Democratic Republic.

This is the first report of the molecular epidemiology of Staphylococcus aureus from skin and soft tissue infections (SSTI) in Laos. We selected a random sample of 96 S. aureus SSTI isolates received by the Microbiology Laboratory, Mahosot Hospital, Vientiane, between July 2012 and June 2014, including representation from seven referral hospitals. Isolates underwent susceptibility testing by Clinical and Laboratory Standards Institute methods, spa typing and DNA microarray analysis, with whole genome sequencing for rare lineages. Median patient age was 19.5 years (interquartile range 2-48.5 years); 52% (50) were female. Forty-three spa types, representing 17 lineages, were identified. Fifty-eight percent (56) of all isolates encoded Panton-Valentine leukocidin (PVL), representing six lineages: half of these patients had abscesses and three had positive blood cultures. The dominant lineage was CC121 (39; 41%); all but one isolate encoded PVL and 49% (19) were from children under five. Staphyococcus argenteus was identified in six (6%) patients; mostly adults > 50 years and with diabetes. Six isolates (6%) belonged to rare lineage ST2885; two possibly indicate cross-infection in a neonatal unit. One isolate from a previously undescribed lineage, ST1541, was identified. Antibiotic resistance was uncommon except for penicillin (93; 97%) and tetracycline (48; 50%). Seven (7%) isolates were methicillin-resistant S. aureus (MRSA), belonging to ST239-MRSA-III, CC59-MRSA-V(T) Taiwan Clone, ST2250-MRSA-IV, ST2885-MRSA-V and CC398-MRSA-V. Globally widespread CC5 and CC30 were absent. There are parallels in S. aureus molecular epidemiology between Laos and neighboring countries and these data highlight the prominence of PVL and suggest infiltration of MRSA clones of epidemic potential from surrounding countries

Adrenal suppression in patients taking inhaled glucocorticoids is highly prevalent and management can be guided by morning cortisol

Context Up to 3% of US and UK populations are prescribed glucocorticoids (GC). Suppression of the hypothalamo–pituitary–adrenal axis with the potential risk of adrenal crisis is a recognized complication of therapy. The 250 μg short Synacthen stimulation test (SST) is the most commonly used dynamic assessment to diagnose adrenal insufficiency. There are challenges to the use of the SST in routine clinical practice, including both the staff and time constraints and a significant recent increase in Synacthen cost. Methods We performed a retrospective analysis to determine the prevalence of adrenal suppression due to prescribed GCs and the utility of a morning serum cortisol for rapid assessment of adrenal reserve in the routine clinical setting. Results In total, 2773 patients underwent 3603 SSTs in a large secondary/tertiary centre between 2008 and 2013 and 17.9% (n=496) failed the SST. Of 404 patients taking oral, topical, intranasal or inhaled GC therapy for non-endocrine conditions, 33.2% (n=134) had a subnormal SST response. In patients taking inhaled GCs without additional GC therapy, 20.5% (34/166) failed an SST and suppression of adrenal function increased in a dose-dependent fashion. Using receiver operating characteristic curve analysis in patients currently taking inhaled GCs, a basal cortisol ≥348 nmol/l provided 100% specificity for passing the SST; a cortisol value <34 nmol/l had 100% sensitivity for SST failure. Using these cut-offs, 50% (n=83) of SSTs performed on patients prescribed inhaled GCs were unnecessary. Conclusion Adrenal suppression due to GC treatment, particularly inhaled GCs, is common. A basal serum cortisol concentration has utility in helping determine which patients should undergo dynamic assessment of adrenal function

Overfeeding, Autonomic Regulation and Metabolic Consequences

The autonomic nervous system plays an important role in the regulation of body processes in health and disease. Overfeeding and obesity (a disproportional increase of the fat mass of the body) are often accompanied by alterations in both sympathetic and parasympathetic autonomic functions. The overfeeding-induced changes in autonomic outflow occur with typical symptoms such as adiposity and hyperinsulinemia. There might be a causal relationship between autonomic disturbances and the consequences of overfeeding and obesity. Therefore studies were designed to investigate autonomic functioning in experimentally and genetically hyperphagic rats. Special emphasis was given to the processes that are involved in the regulation of peripheral energy substrate homeostasis. The data revealed that overfeeding is accompanied by increased parasympathetic outflow. Typical indices of vagal activity (such as the cephalic insulin release during food ingestion) were increased in all our rat models for hyperphagia. Overfeeding was also accompanied by increased sympathetic tone, reflected by enhanced baseline plasma norepinephrine (NE) levels in both VMH-lesioned animals and rats rendered obese by hyperalimentation. Plasma levels of NE during exercise were, however, reduced in these two groups of animals. This diminished increase in the exercise-induced NE outflow could be normalized by prior food deprivation. It was concluded from these experiments that overfeeding is associated with increased parasympathetic and sympathetic tone. In models for hyperphagia that display a continuously elevated nutrient intake such as the VMH-lesioned and the overfed rat, this increased sympathetic tone was accompanied by a diminished NE response to exercise. This attenuated outflow of NE was directly related to the size of the fat reserves, indicating that the feedback mechanism from the periphery to the central nervous system is altered in the overfed state.

Segond's fracture: a biomechanical cadaveric study using navigation

Background Segond’s fracture is a well-recognised radiological sign of an anterior cruciate ligament (ACL) tear. While previous studies evaluated the role of the anterolateral ligament (ALL) and complex injuries on rotational stability of the knee, there are no studies on the biomechanical effect of Segond’s fracture in an ACL deficient knee. The aim of this study was to evaluate the effect of a Segond’s fracture on knee rotation stability as evaluated by a navigation system in an ACL deficient knee. Materials and methods Three different conditions were tested on seven knee specimens: intact knee, ACL deficient knee and ACL deficient knee with Segond’s fracture. Static and dynamic measurements of anterior tibial translation (ATT) and axial tibial rotation (ATR) were recorded by the navigation system (2.2 OrthoPilot ACL navigation system B. Braun Aesculap, Tuttlingen, Germany). Results Static measurements at 30 showed that the mean ATT at 30 of knee flexion was 5.1 ± 2.7 mm in the ACL intact condition, 14.3 ± 3.1 mm after ACL cut (P = 0.005), and 15.2 ± 3.6 mm after Segond’s fracture (P = 0.08). The mean ATR at 30 of knee flexion was 20.7 ± 4.8 in the ACL intact condition, 26.9 ± 4.1 in the ACL deficient knee (P[0.05) and 30.9 ± 3.8 after Segond’s fracture (P = 0.005). Dynamic measurements during the pivot-shift showed that the mean ATT was 7.2 ± 2.7 mm in the intact knee, 9.1 ± 3.3 mm in the ACL deficient knee(P = 0.04) and 9.7 ± 4.3 mm in the ACL deficient knee with Segond’s fracture (P = 0.07). The mean ATR was 9.6 ± 1.8 in the intact knee, 12.3 ± 2.3 in the ACL deficient knee (P[0.05) and 19.1 ± 3.1 in the ACL deficient knee with Segond’s lesion (P = 0.016). Conclusion An isolated lesion of the ACL only affects ATT during static and dynamic measurements, while the addition of Segond’s fracture has a significant effect on ATR in both static and dynamic execution of the pivot-shift test, as evaluated with the aid of navigation