10 research outputs found
Spontaneous Sigmoid Colon Rupture Secondary to Endometriosis in the Third Trimester of Pregnancy
Antimicrobial activity of heterotrophic bacterial communities from the marine sponge Erylus discophorus (Astrophorida, Geodiidae)
10 páginas, 3 tablas.Heterotrophic bacteria associated with two specimens of the marine sponge Erylus discophorus were screened for their
capacity to produce bioactive compounds against a panel of human pathogens (Staphylococcus aureus wild type and
methicillin-resistant S. aureus (MRSA), Bacillus subtilis, Pseudomonas aeruginosa, Acinetobacter baumanii, Candida albicans
and Aspergillus fumigatus), fish pathogen (Aliivibrio fischeri) and environmentally relevant bacteria (Vibrio harveyi). The
sponges were collected in Berlengas Islands, Portugal. Of the 212 isolated heterotrophic bacteria belonging to Alpha- and
Gammaproteobacteria, Actinobacteria and Firmicutes, 31% produced antimicrobial metabolites. Bioactivity was found
against both Gram positive and Gram negative and clinically and environmentally relevant target microorganisms.
Bioactivity was found mainly against B. subtilis and some bioactivity against S. aureus MRSA, V. harveyi and A. fisheri. No
antifungal activity was detected. The three most bioactive genera were Pseudovibrio (47.0%), Vibrio (22.7%) and Bacillus
(7.6%). Other less bioactive genera were Labrenzia, Acinetobacter, Microbulbifer, Pseudomonas, Gordonia, Microbacterium,
Micrococcus and Mycobacterium, Paenibacillus and Staphylococcus. The search of polyketide I synthases (PKS-I) and
nonribosomal peptide synthetases (NRPSs) genes in 59 of the bioactive bacteria suggested the presence of PKS-I in 12
strains, NRPS in 3 strains and both genes in 3 strains. Our results show the potential of the bacterial community associated
with Erylus discophorus sponges as producers of bioactive compounds.This research was supported by the European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Programme
and national funds through FCT – Foundation for Science and Technology, under the projects PEst-C/MAR/LA0015/2013, PEst-OE/QUI/UI0612/2011 and PTDC/
QUI-QUI/098053/2008. Ana Patrícia Graçaa acknowledges an ERAMUS fellowship. Joana Bondoso was financed by FCT - Foundation for Science and Technology
(PhD grant SFRH/BD/35933/2007). Joana R. Xavier’s research is funded by an FCT - Foundation for Science and Technology postdoctoral fellowship (grant
no. SFRH/BPD/62946/2009). In addition, this work was funded by the Fundación MEDINA, a public-private partnership of Merck Sharp and Dohme of España/
Universidad de Granada/Junta de AndalucíaPeer reviewe
Comparison of Cold-Knife Conization versus Loop Electrosurgical Excision for Cervical Adenocarcinoma In Situ (ACIS): A Systematic Review and Meta-Analysis
Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA
Because of the important roles noncoding RNAs play in gene expression, their sequence-specific recognition is important for both fundamental science and the pharmaceutical industry. However, most noncoding RNAs fold in complex helical structures that are challenging problems for molecular recognition. Herein, we describe a method for sequence-specific recognition of double-stranded RNA using peptide nucleic acids (PNAs) that form triple helices in the major grove of RNA under physiologically relevant conditions. We also outline methods for solid-phase conjugation of PNA with cell-penetrating peptides and fluorescent dyes. Protocols for PNA preparation and binding studies using isothermal titration calorimetry are described in detail
Oncological and reproductive outcomes of adenocarcinoma in situ of the cervix managed with the loop electrosurgical excision procedure
Toxoplasma gondii-induced host cellular cell cycle dysregulation is linked to chromosome missegregation and cytokinesis failure in primary endothelial host cells
SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150
Antimicrobial Activity of Heterotrophic Bacterial Communities from the Marine Sponge Erylus discophorus (Astrophorida, Geodiidae)
Adenosine Signaling in the Tumor Microenvironment
Adenosine, deriving from ATP released by dying cancer cells and then degradated in the tumor environment by CD39/CD73 enzyme axis, is linked to the generation of an immunosuppressed niche favoring the onset of neoplasia. Signals delivered by extracellular adenosine are detected and transduced by G-protein-coupled cell surface receptors, classified into four subtypes: A1, A2A, A2B, and A3. A critical role of this nucleoside is emerging in the modulation of several immune and nonimmune cells defining the tumor microenvironment, providing novel insights about the development of novel therapeutic strategies aimed at undermining the immune-privileged sites where cancer cells grow and proliferate