Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India

Artemisia which produces a large number of secondary metabolites is naturally found in cold desert high altitude environment of India. Secondary metabolites such as alkaloids, flavonoids, phenols, polysaccharides and terpenes represent a significant barrier to the extraction of pure genomic DNA. Thus, in this study, the DNA extraction protocol to extract pure genomic DNA from different Artemisia species was tailored. The protocol was based on the CTAB method with slight modifications. In the study, 1.6 M NaCl, 2% cetyltrimethyl ammonium bromide (CTAB), 3% polyvinylpyrrolidone (PVP) and 0.5% β-mercaptoethanol was used in the extraction buffer. The incubation period was kept for 1 h at 65°C with one-tenth of the volume of warm (55°C) 10% CTAB solution during the extraction process. This study described a reliable protocol for extracting good quality and optimum amount of DNA from Artemisia species suitable for PCR analysis.Key words: Artemisia, genomic DNA isolation, PCR amplification, secondary metabolites

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Тематика курсовых работ (дневное отделение)

The use of treated wastewater (TWW) as an alternative resource to fresh water (FW) for irrigation purposes is becoming increasingly important, especially in semiarid and arid regions. However, achieving success in crop production largely depends on the adoption of appropriate on‐farm management strategies aimed at optimizing crop yields, maintaining soil productivity and safeguarding the environment. For this purpose, predictive models are of particular interest. A safe irrigation management (SIM) model developed and tested in this research was used to schedule irrigation under controlled management tailored to the use of 1) TWW and 2) FW and to reproduce farmers' strategies. These management strategies aim to improve actual irrigation practices, accounting for water quality, soil characteristics, and crop yield. The results of the application of SIM on a citrus farm in Souss‐Massa, Morocco, show that the management strategy adopted by farmers withdraws considerable amounts of water and results in substantial drainage volumes compared to those in the SIM strategy. In the specific case of TWW, the strategy simulated by the SIM model resulted in a decrease in yield of approximately 4%, compared to the 23% decrease derived from the farmers' traditional strategy. Moreover, SIM allowed for great savings in terms of fertilizing elements and for the reduction in the movement of water and salts beyond the root zone, usually considered the main source of groundwater contamination. These results confirm the appropriateness of using prediction models and the accuracy of the SIM model in adapting irrigation strategies to TWW, which will be an integral part of the strategies that encourage their use in irrigated agriculture