325 research outputs found
In vitro modelling of alveolar repair at the air-liquid interface using alveolar epithelial cells derived from human induced pluripotent stem cells
Research on acute and chronic lung diseases would greatly benefit from reproducible availability of alveolar epithelial cells (AEC). Primary alveolar epithelial cells can be derived from human lung tissue but the quality of these cells is highly donor dependent. Here, we demonstrated that culture of EpCAM+ cells derived from human induced pluripotent stem cells (hiPSC) at the physiological air-liquid interface (ALI) resulted in type 2 AEC-like cells (iAEC2) with alveolar characteristics. iAEC2 cells expressed native AEC2 markers (surfactant proteins and LPCAT-1) and contained lamellar bodies. ALI-iAEC2 were used to study alveolar repair over a period of 2 weeks following mechanical wounding of the cultures and the responses were compared with those obtained using primary AEC2 (pAEC2) isolated from resected lung tissue. Addition of the Wnt/β-catenin activator CHIR99021 reduced wound closure in the iAEC2 cultures but not pAEC2 cultures. This was accompanied by decreased surfactant protein expression and accumulation of podoplanin-positive cells at the wound edge. These results demonstrated the feasibility of studying alveolar repair using hiPSC-AEC2 cultured at the ALI and indicated that this model can be used in the future to study modulation of alveolar repair by (pharmaceutical) compounds
Deltamethrin residues applied in different formulations in staked cucumber and the actions of insecticides on the pickleworm control
Vortices on Orbifolds
The Abelian and non-Abelian vortices on orbifolds are investigated based on
the moduli matrix approach, which is a powerful method to deal with the BPS
equation. The moduli space and the vortex collision are discussed through the
moduli matrix as well as the regular space. It is also shown that a quiver
structure is found in the Kahler quotient, and a half of ADHM is obtained for
the vortex theory on the orbifolds as the case before orbifolding.Comment: 25 pages, 4 figures; references adde
Development of porous and flexible ptmc membranes for in vitro organ models fabricated by evaporation-induced phase separation
Polymeric membranes are widely applied in biomedical applications, including in vitro
organ models. In such models, they are mostly used as supports on which cells are cultured to create
functional tissue units of the desired organ. To this end, the membrane properties, e.g., morphology
and porosity, should match the tissue properties. Organ models of dynamic (barrier) tissues, e.g.,
lung, require flexible, elastic and porous membranes. Thus, membranes based on poly (dimethyl
siloxane) (PDMS) are often applied, which are flexible and elastic. However, PDMS has low cell
adhesive properties and displays small molecule ad- and absorption. Furthermore, the introduction
of porosity in these membranes requires elaborate methods. In this work, we aim to develop porous
membranes for organ models based on poly(trimethylene carbonate) (PTMC): a flexible polymer
with good cell adhesive properties which has been used for tissue engineering scaffolds, but not in
in vitro organ models. For developing these membranes, we applied evaporation-induced phase
separation (EIPS), a new method in this field based on solvent evaporation initiating phase separation,
followed by membrane photo-crosslinking. We optimised various processing variables for obtaining
form-stable PTMC membranes with average pore sizes between 5 to 8 µm and water permeance in
the microfiltration range (17,000–41,000 L/m2
/h/bar). Importantly, the membranes are flexible and are
suitable for implementation in in vitro organ models
Avaliação clínica a longo prazo - pelo sinal de Phalen, Tinel e parestesia noturna - dos pacientes submetidos a cirurgia de liberação do túnel do carpo com instrumento de Paine®
Screening of patients with augmented renal clearance in ICU: taking into account the CKD-EPI equation, the age, and the cause of admission
Saúde dos trabalhadores de enfermagem e a segurança do paciente: o olhar de gerentes de enfermagem*
The rainy season increases the abundance and richness of the aquatic insect community in a Neotropical reservoir
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