How I treat transfusional iron overload

Mammary organoids from adult mice produce tubules, analogous to mammary ducts in vivo, in response to hepatocyte growth factor (HGF) when cultured in collagen gels. The combination of HGF plus progestin (R5020) causes reduced tubule number and length. We hypothesized that the inhibitory effect on tubulogenesis was due to progestin-mediated alteration of HGF/c-Met signaling. Using molecular inhibitors and short hairpin RNA, it was determined that HGF activation of Ras-related C3 botulinum toxin substrate (Rac1) was required for the formation of cytoplasmic extensions, the first step of tubulogenesis, and that Rac1 activity was Src kinase (Src) and focal adhesion kinase (FAK) dependent. The highly novel finding was that R5020 reduced tubulogenesis by up-regulating and increasing extracellular laminin and \u3b16-integrin ligation to reduce activation of the Src, focal adhesion kinase, and Rac1 pathway. Receptor activator of nuclear factor-\u3baB ligand, another progesterone-induced paracrine factor, did not replicate this effect of R5020. The inhibitory effect of R5020 on tubulogenesis was likely mediated through progesterone receptor (PR) isoform A (PRA), because PRA is the predominant PR isoform expressed in the organoids, and the progestin-induced effect was prevented by the PR antagonist RU486. These results provide a plausible mechanism that explains progestin/PRA-mediated blunting of HGF-induced tubulogenesis in vitro and is proposed to be relevant to progesterone/PRA- induced side-branching in vivo during pregnancy. Copyrigh

Gastro-intestinal flora and diarrhoea after Vagotomy

Twenty patients, 7 of whom had diarrhoea after vagotomy and drainage, were  investigated by culture of gastric and jejunal aspirates and measurements of faecal  fat, free bile acids in jejunal fluid, urinary indican excretion, serum folate and serum vitamin B,2. The haematological findings were compared with those in 20 patients  with duodenal ulcer who had not undergone surgery and in 25 healthy controls. The pattern of small bowel flora was normal after vagotomy; this was consistent where the normal results of the urinary indican estimations. There was no relationship between diarrhoea and the bacteriological results. Steatorrhoea occurred in 6 of the 7 patients with  diarrhoea and in only 1 of 13 without diarrhoea, suggesting a relationship between diarrhoea and faecal fat excretion after vagotomy and drainage. The mean serum folate and vitamin B'2 levels of the patients after vagotomy were significantly lower than thosein healthy controls. Some of the folate levels in the duodenal ulcer controls were subnormal

Kapita Selekta Hematologi

IX,419 HLM. ; 21X27 C

Kapita selekta hematologi

ix,419 hlm.;21x27 c

Kapita selekta hematologi (essential haematology), ed.6/ Hoffbrand

ix, 419 hal.: ill, tab.; 30 cm

Kapita selekta haematologi = Essential haematologi ed.2/ Hoffbrand

x, 266 hal.: ill.; 22 cm

Kapita selekta hematologi (essential haematology), ed.6/ Hoffbrand

ix, 419 hal.: ill, tab.; 30 cm

Kapita selekta hematologi (essential haematology), ed.6/ Hoffbrand

ix, 419 hal.: ill, tab.; 30 cm