The modern principles in treatment of Legg – Calve – Perthes syndrome in children

The aim: to improve the results of treatment of degenerative hip’s diseases in children by means of pathogenetic pattern. Methods and Materials. 56 children with Legg — Calve — Perthes syndrome aged from 4 to 12 years and 20 children of control group without hip’s diseases have been studied. Results. The modern principles of surgical treatment of Legg — Calve — Perthes syndrome in children have been identified. Conclusion. The results of surgical treatment of patients with a different degree of pathological process depending on a type of surgery (osteoplastic surgery, formation of vascularizing autotransplant, varus osteotomy, rotational intertrochanteric osteotomy) have been presented

ERRATUM TO: Power corrections 1/Q21/Q^2 to parton sum rules for deep inelastic scattering from polarized targets

We clarify conflicting results in the literature on coefficient functions in front of higher twist operators contributing to the parton sum rules for deep inelastic scattering from polarized targets. The necessary corrections do not affect our calculations of matrix elements, published in Phys.Lett.B242(1990)245, but change final estimates of the $\sim 1/Q^2$ contributions to Bjorken and Ellis--Jaffe sum rules.Comment: 2 pages, MPI-Ph/93-0

Безфидерная культура эпителия слизистой губы человека для тканевой инженерии и регенеративной медицины

Background.The cultured cheek mucosa epithelium (buccal epithelium, BE) is used for autologous transplants generation and tissue engineering. An alternative source of cells for these purposes may be the lip mucosa, covered, like BE, with non-keratinized stratified squamous epithelium, but with some histological distinctions.Aims to characterize the human lip mucosa as a promising source of epithelial cells for autologous transplantation and tissue engineering.Methods.Scrapings of the lip, cheek, and gum mucosa from five healthy volunteers were analyzed by cytofluorimetry to determine the level of desquamation and cytokeratin (CK) 10 and 13 expression. The lip mucosa of two patients was characterized using routine histological staining and fluorescence immunohistochemistry for CK 3, 4, 10, 13, and p63 marker. 35 samples of full-thickness strips of the patients lip mucosa were used to set the explant (n=18) and enzymatic (n=17) techniques for expansion epithelium. Culture systems with 1.05 and 0.06mM Calcium contained 5% fetal bovine serum, 5 g/ml human insulin, 5 g/ml hydrocortisone, 10 ng/ml human epidermal growth factor. Stable cultures were stained for p63, vimentin, zonula occludens-1 (ZO-1), and CK10. Software tools determined levels of their expression.Results.The number of cells in the lip and gum samples was significantly lower than from the cheek. The median number of CK13 positive cells was significantly different for the gum (6.4%) and cheek (64.8%, p=0.0089). Significant differences for CK10 positive cells were not observed. The epithelium of the lip mucosa was 72.13.6 m thick, relatively flat, and without keratinization sites. Samples were positively stained for CK 4 and 13, in the absence of expression of CK 3 and 10. The primary culture of epithelial cells obtained by explant technique was significantly more effective (p=0.001) in comparison with the enzymatic method. Stable cultures had a cobble-stone morphology in both culture systems. The levels of vimentin and p63 expression in both culture systems was not significantly differ. ZO-1 expression was 3.6-fold higher for 1.05-mMCa++medium (p=0.0006).Conclusions.Epithelium cell culture from the lip mucosa can be obtained by culturing explants without a feeder layer. Quality control steps have been developed for cultured cells and biopsy site.Обоснование.Культивированный эпителий слизистой щеки (буккальный эпителий, БЭ) используетсядлясоздания аутологичных трансплантатовитканевой инженерии. Альтернативным источником клетокдляэтих целей может являться слизистая губы, покрытая,какиБЭ, неороговевающим многослойным плоским эпителием,ноимеющая некоторые гистологические отличия.Цель исследования охарактеризовать эпителий слизистой губы человекакакперспективный источник эпителиальных клетокдляаутологичной трансплантацииитканевой инженерии.Методы.Цитофлуориметрия соскобов слизистой губы, щекиидесны была выполненапооригинальному протоколуу5 здоровых добровольцевдляопределения уровня десквамации поверхностных клетокиэкспрессии цитокератинов (CK) 10и13. Слизистая губыотдвух пациентов была охарактеризованаспомощью рутинной гистологической окраскиифлуоресцентной иммуногистохимиинаCK 3, 4, 10, 13ипролиферативный маркер p63. 35 образцов полнослойного лоскута слизистой губыотпациентов были использованыдляпостановки эксплантной (n=18)иферментативной (n=17) техник выделения первичной культуры эпителия. Культуральные системыс1,05и0,06 мМ кальция содержали 5% фетальной бычьей сыворотки, 5 мкг/мл инсулина растворимого человеческого, 5 мкг/мл гидрокортизонаи10 нг/мл человеческого эпидермального фактора роста. Стабильные культуры эпителия были окрашенынамаркер p63, виментин, белок плотных межклеточных контактов (ZO-1)иCK 10, уровни экспрессии которых были определены программными средствами.Результаты.Поданным цитофлуориметрии число клетоквсоскобахсгубыидесны было достоверно ниже, чем со щеки. Число CK13-позитивных клетокпомедиане достоверно отличалосьдлясоскобовсдесны (6,4%)исо щеки (64,8%, р=0,0089). Достоверных отличийпоCK10-позитивным клеткамвсоскобахнеотмечали. Эпителий слизистой губы толщиной 72,13,6 мкм был представлен неороговевающим многослойным плоским,безучастков кератинизациииположительно окрашивалсянамаркеры СК 4и13вотсутствие экспрессии СК 3и10. Первичная культура клеток эпителия, полученная путем культивирования эксплантов, была достоверно более результативна (p=0,001)всравнениисферментативным способом. Стабильные культуры имели характерную морфологиювобеих культуральных системах. Уровень экспрессии виментинаиядерного транскрипционного фактора р63вкультуральных системах достовернонеотличался. Маркер ZO-1 былв3,6 раза более выраженприкультивированиивсредес1,05 мМ Ca++(p=0,0006).Заключение.Культура клеток эпителия слизистой губы может быть получена путем культивирования эксплантовбезфидерного слоя. Разработаны этапы контроля качествадлякультивированных клетокиучастка биопсии

Some studies on the deformation of the membrane in an RF MEMS switch

Radio Frequency (RF) switches of Micro Electro Mechanical Systems (MEMS) are appealing to the mobile industry because of their energy efficiency and ability to accommodate more frequency bands. However, the electromechanical coupling of the electrical circuit to the mechanical components in RF MEMS switches is not fully understood. In this paper, we consider the problem of mechanical deformation of electrodes in RF MEMS switch due to the electrostatic forces caused by the difference in voltage between the electrodes. It is known from previous studies of this problem, that the solution exhibits multiple deformation states for a given electrostatic force. Subsequently, the capacity of the switch that depends on the deformation of electrodes displays a hysteresis behaviour against the voltage in the switch. We investigate the present problem along two lines of attack. First, we solve for the deformation states of electrodes using numerical methods such as finite difference and shooting methods. Subsequently, a relationship between capacity and voltage of the RF MEMS switch is constructed. The solutions obtained are exemplified using the continuation and bifurcation package AUTO. Second, we focus on the analytical methods for a simplified version of the problem and on the stability analysis for the solutions of deformation states. The stability analysis shows that there exists a continuous path of equilibrium deformation states between the open and closed state

The Accidental Terrorist: Okhrana Connections to the Extreme-Right and the Attempt to Assassinate Sergei Witte in 1907

This article represents a case study in the relationship between the tsarist secret police (commonly known as the Okhrana in the West and okhranka in Russia) and acts of political terror perpetrated by the extreme-right in late imperial Russia. This specific case concerns the tangled web of conspiracy, propaganda and controversy that surrounded the attempted assassination of former-Chairman of the Council of Ministers, Sergei Witte, in 1907

On the free field realization of the osp(1|2) current algebra

The free field representation of the osp(1|2) current algebra is analyzed. The four point conformal blocks of the theory are studied. The structure constants for the product of an arbitrary primary operator and a primary field that transforms according to the fundamental representation of osp(1|2) are explicitly calculated.Comment: 11 pages, phyzz

Exact noncommutative solitons in p-adic strings and BSFT

The tachyon field of p-adic string theory is made noncommutative by replacing ordinary products with noncommutative products in its exact effective action. The same is done for the boundary string field theory, treated as the p -> 1 limit of the p-adic string. Solitonic lumps corresponding to D-branes are obtained for all values of the noncommutative parameter theta. This is in contrast to usual scalar field theories in which the noncommutative solitons do not persist below a critical value of theta. As theta varies from zero to infinity, the solution interpolates smoothly between the soliton of the p-adic theory (respectively BSFT) to the noncommutative soliton.Comment: 1+14 pages (harvmac b), 1 eps figure, v2: references added, typos correcte

Challenges of Matrix Models

Brief review of concepts and unsolved problems in the theory of matrix models.Comment: Contribution to Proceedings of Cargese 200