12 research outputs found
Histologic, histomorphometric and ultrastructural evaluation of titanium particles detached from titanium plasma sprayed endosseous dental implants.
TITANIUM ORTHOPAEDIC IMPLANT SURFACES INFLUENCE OSTEOBLAST CELLS RESPONSE
none8noneD.Martini; S.Guizzardi; M.Raspanti; C.Galli; A.Trirè; E.Orsini; M.Quaranta; A.RuggeriD.Martini; S.Guizzardi; M.Raspanti; C.Galli; A.Trirè; E.Orsini; M.Quaranta; A.Rugger
Presence of titanium granules in periimplant tissues: influence of implant surface roughness
\u201cIn vitro\u201d and \u201cin vivo\u201d studies of titanium implant surface microtopography
Destination of titanium particles detached from titanium plasma sprayed endosseous dental implants
none8noneE.Orsini; M.Franchi; D.Martini; B.Bacchelli; A.Trirè; M.Quaranta; M.Fini; A.RuggeriE.Orsini; M.Franchi; D.Martini; B.Bacchelli; A.Trirè; M.Quaranta; M.Fini; A.Rugger
Titanium implants surface micromorphology in \u201cin vitro\u201d and \u201cin vivo\u201d studies
Immunohistochemical and biochemical assay of MMP-3 in human dentine.
Objective
The function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays.
Methods
Powdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H3PO4 for 10 min or (2) untreated (control). The presence of MMP-3 was measured using a colorimetric assay system (QuantiSirâ„¢, Epigentek, USA). Additional cryo-fractured dentine fragments were processed for immunohistochemical identification of MMP-3 under FEI-SEM. Casein-zymography was used to investigate MMP-3 activity.
Results
MMP-3 detected level was 2.732 ng/μL in partially demineralised dentine powder, whilst it increased to 3.280 ng/μL in mineralised dentine. The FEI-SEM analysis revealed positive immunolabelling patterns for MMP-3, predominantly localized on the intertubular collagen fibrillar network showing MMP-3 directly or indirectly bound to the collagen fibrils. Casein-zymograms showed positive proteolytic activity for MMP-3 in demineralised dentine powder.
Conclusion
The results of the study clearly revealed the presence and distribution of MMP3 in human sound dentine. Whilst the presence was verified, its role is still unclear. Future studies are needed to investigate the possible involvement of MMP-3 in physiological and pathological condition of the dentine–pulp complex