Tissue reconstruction of abdominal wall with butyric acid-based nets: preliminary in vitro test using tissue engineering strategies

OBJECTIVE: A hernia of the abdominal wall is an opening of the muscles in the abdominal wall, which is frequently treated via the application of a surgical mesh. The purpose of this research is to study how human adipose-derived stem cells (hADSCs) interact with Phasix™ Mesh, a commercially available mesh for hernia repair. Studying how cells derived from the abdominal region behave with Phasix™ Mesh is crucial to improve the state of the art of current surgery and achieve effective tissue restoration. MATERIALS AND METHODS: hADSCs were seeded onto Phasix™ Mesh, a fully resorbable surgical mesh of poly (4-hydroxybutyric acid) (P4HB). Cell viability was assessed through MTT assay, and cell growth and adhesion were evaluated via multiple imaging techniques and gene imaging profiling. RESULTS: Results confirm that the nets support cells proliferation, extracellular matrix production and increasing of angiogenetic factor. CONCLUSIONS: Butyric acid-based nets are promising scaffolds for abdominal wall reconstruction

Analysis and comparison of quality of life and patients’ satisfaction between dental-skeletal dysmorphisms and Obstructive Sleep Apnea (OSA) patients following orthognathic surgery

OBJECTIVE: The aim of this study was to evaluate the impact of orthognathic surgery on the patients’ satisfaction and quality of life (QoL) in patients with dental skeletal dysmorphisms and Obstructive Sleep Apnea (OSA). SUBJECTS AND METHODS: Patients were grouped into two cohorts, patients with OSA (G1) and patients with dento-skeletal dysmorphisms (G2). SF-36 questionnaire was obtained from all subjects before interventions. A standardized follow-up protocol, including a second SF-36 questionnaire was planned as at least 6 months after surgery. The impact of surgery on satisfaction was evaluated by post-operative patient satisfaction-based survey. RESULTS: 61 patients were included as: 21 OSA (G1), 12 Class II (G2-a) and 28 Class III (G2-b) patients. The mean post-operative follow-up was 65.47±26.36 months. In the SF-36 results, when pre and post operative surveys were compared, the quality of life increased significantly for G1 in all items except for body pain. In G2, when pre and post operative surveys were compared, the quality of life increased significantly in items related to emotional well-being, health transition, role limitations due to emotional problems, while other parameters did not significantly change. When groups were compared, there was no difference among them except for physical functioning which was improved for OSA patients. According to the Rustemeyer results, overall post-operative satisfaction score was 84.92±14.72%. There was a significant difference for patient satisfaction considering facial aesthetics in both groups. For chewing function there was no difference for patient satisfaction in G1, but there was a significant difference in G2 patients. CONCLUSIONS: Orthognathic surgery seems to be beneficial in terms of patients’ satisfaction and patients’ satisfaction for both dental skeletal dysmorphism and OSA patients

Malocclusioni di classe II prima divisione: considerazioni sulla scelta di eseguire una o due fasi di trattamento

Objectives: To determine the appropriate timing for treatment of Class II Division 1 malocclusion. Materials and methods: We conducted a review of the literature to analyze the controversy surrounding the use single-phase versus two-phase orthodontic treatment for this type of malocclusion. Results: The evidence shows that early orthodontic treatment is effective in specific clinical situations, but in many other cases, later single-phase treatment is more appropriate. Conclusions: The practicing orthodontist should tailor treatment timing to the specific clinical situation

Cephalometric evaluation of ocular protrusion at stages of growth

The sagittal relationships between the orbital globe and the ocular orbit have great importance in aesthetic and functional evaluations. These relationships are useful in diagnosis and treatment of some malformations in which the orbital volume is reduced such as craniostenosis. The aim of the present study was the cephalometric evaluation of the ocular protrusion at different growth stages. Ninety-two patients with negative history of craniosynostosis, severe myopia, and skeletal class I were selected for the present study. Lateral teleradiography x-ray was performed for all the patients, and 5 groups were carried out based on the dentition phase. Cephalometric analyses were performed on the lateral teleradiography x-ray. Mean values of ocular protrusion (B-Or post) for females and males were found and digitized for the 5 groups tested. All other sagittal and vertical measurements were successfully computed by the trained operator. B-Or post showed values between -14.93 mm and -16.90 mm among the groups. The cephalometric analysis proposed in the present study allowed to successfully evaluate sagittal and vertical relationship of the orbital globe and the orbital cavity in growing patients. Moreover, the B-Or post maintained its value during growth in the present study

D-Bite (Digital Bite): CAD/CAM manufacturing technique = D-Bite (Digital-Bite): Tecnica di costruzione CAD/CAM]

Objectives To manufacture an occlusal splint based on CAD/CAM technology. Materials and methods Plaster casts made from oral impressions were scanned with CAD/CAM technology. The CAD/CAM device employed was a structured light-based scanner. CAD generates a 3D digital image that can be used to design the splint. During the CAM phase, the D-Bite (Digital Bite) was created from a block of polymethyl methacrylate (PMMA) with a thickness of about 20 mm. Results The production of a digitally designed D-Bite (Digital Bite). The D-Bite manufacturing technique is standardized thanks to computer-assisted procedures. Conclusions The D-Bite manufactured with CAD/CAM technology provided excellent biological and esthetic results, and the manufacturing technique was simple and rapi

Sublingual ranula : a closer look to its surgical management

Ranulas have been managed by various surgical methods, and the optimal treatment is still controversial. The aim of this study was to analyze a group of 124 surgically treated patients with intraoral ranula to assess 3 different methods: sublingual gland removal combined with the ranula excision, conventional marsupialization, and a variant of the marsupialization technique usually performed in our departments. Recurrence rate was 0% after radical treatment, 25.8% after marsupialization, and 12% after modified marsupialization. We suggest that conservative methods should always be considered as treatment of superficial oral ranulas. The modification of the conventional marsupialization by suturing the edges of the pseudocyst before unroofing of the lesion was demonstrated to be a useful technical strategy that simplifies and accelerates the surgical procedures and probably contributed to preventing recurrences

Comparison of conventional orthognathic surgery and "surgery-first" protocol: a new weapon against time

The aim of this literature review article is to evaluate the new surgical technique "Surgery-First" and compare it with the conventional orthognathic surgery in terms of treatment length, patient satisfaction and post-surgical stability. The goal is to compare the pro and cons of the two techniques and to determine which technique can offer more efficient results