On Minimal N=4 Topological Strings And The (1,k) Minimal Bosonic String

In this paper we consider tree-level scattering in the minimal N=4 topological string and show that a large class of N-point functions can be recast in terms of corresponding amplitudes in the (1,k) minimal bosonic string. This suggests a non-trivial relation between the minimal N=4 topological strings, the (1,k) minimal bosonic strings and their corresponding ADE matrix models. This relation has interesting and far-reaching implications for the topological sector of six-dimensional Little String Theories.Comment: lanlmac, 30 pages; v3 minor revisions, version published in JHE

On the Connection between N=2 Minimal String and (1,n) Bosonic Minimal String

We study the scattering amplitudes in the N=2 minimal string or equivalently in the N=4 topological string on ALE spaces. We find an interesting connection between the tree level amplitudes of the N=2 minimal string and those of the (1,n) minimal bosonic string. In particular we show that the four and five-point functions of the N=2 string can be directly rewritten in terms of those of the latter theory. This relation offers a map of physical states between these two string theories. Finally we propose a possible matrix model dual for the N=2 minimal string in the light of this connection.Comment: 40 pages, one figure, harvmac, minor corrections, references adde

Unitarity Meets Channel-Duality for Rolling / Decaying D-Branes

Investigations for decay of unstable D-brane and rolling of accelerated D-brane dynamics have revealed that various proposed prescriptions give different result for spectral amplitudes and observables. Here, we study them with particular attention to unitarity and open-closed channel duality. From "ab initio" derivation in the open string channel, both in Euclidean and Lorentzian worldsheet approaches, we find heretofore overlooked contribution to the spectral amplitudes and obervables. The contribution is fortuitously absent for decay of unstable D-brane, but is present for rolling of accelerated D-brane. We finally show that the contribution is imperative for ensuring unitarity and optical theorem at each order in string loop expansion.Comment: Latex, 28 pages, 2 figures (colored

A conformational ensemble derived using NMR methyl chemical shifts reveals a mechanical clamping transition that gates the binding of the HU protein to dna

Recent improvements in the accuracy of structure-based methods for the prediction of nuclear magnetic resonance chemical shifts have inspired numerous approaches for determining the secondary and tertiary structures of proteins. Such advances also suggest the possibility of using chemical shifts to characterize the conformational fluctuations of these molecules. Here we describe a method of using methyl chemical shifts as restraints in replica-averaged molecular dynamics (MD) simulations, which enables us to determine the conformational ensemble of the HU dimer and characterize the range of motions accessible to its flexible \u3b2-arms. Our analysis suggests that the bending action of HU on DNA is mediated by a mechanical clamping mechanism, in which metastable structural intermediates sampled during the hinge motions of the \u3b2-arms in the free state are presculpted to bind DNA. These results illustrate that using side-chain chemical shift data in conjunction with MD simulations can provide quantitative information about the free energy landscapes of proteins and yield detailed insights into their functional mechanisms

Cyclophilin a catalyzes proline isomerization by an electrostatic handle mechanism

Proline isomerization is a ubiquitous process that plays a key role in the folding of proteins and in the regulation of their functions. Different families of enzymes, known as "peptidyl-prolyl isomerases" (PPIases), catalyze this reaction, which involves the interconversion between the cis and trans isomers of the N-terminal amide bond of the amino acid proline. However, complete descriptions of the mechanisms by which these enzymes function have remained elusive. We show here that cyclophilin A, one of the most common PPIases, provides a catalytic environment that acts on the substrate through an electrostatic handle mechanism. In this mechanism, the electrostatic field in the catalytic site turns the electric dipole associated with the carbonyl group of the amino acid preceding the proline in the substrate, thus causing the rotation of the peptide bond between the two residues. We identified this mechanism using a combination of NMR measurements, molecular dynamics simulations, and density functional theory calculations to simultaneously determine the cis-bound and trans-bound conformations of cyclophilin A and its substrate as the enzymatic reaction takes place. We anticipate that this approach will be helpful in elucidating whether the electrostatic handle mechanism that we describe here is common to other PPIases and, more generally, in characterizing other enzymatic processes

Global Impact of COVID-19 on Stroke Care and IV Thrombolysis

OBJECTIVE: To measure the global impact of COVID-19 pandemic on volumes of IV thrombolysis (IVT), IVT transfers, and stroke hospitalizations over 4 months at the height of the pandemic (March 1 to June 30, 2020) compared with 2 control 4-month periods. METHODS: We conducted a cross-sectional, observational, retrospective study across 6 continents, 70 countries, and 457 stroke centers. Diagnoses were identified by their ICD-10 codes or classifications in stroke databases. RESULTS: There were 91,373 stroke admissions in the 4 months immediately before compared to 80,894 admissions during the pandemic months, representing an 11.5% (95% confidence interval [CI] -11.7 to -11.3, p < 0.0001) decline. There were 13,334 IVT therapies in the 4 months preceding compared to 11,570 procedures during the pandemic, representing a 13.2% (95% CI -13.8 to -12.7, p < 0.0001) drop. Interfacility IVT transfers decreased from 1,337 to 1,178, or an 11.9% decrease (95% CI -13.7 to -10.3, p = 0.001). Recovery of stroke hospitalization volume (9.5%, 95% CI 9.2-9.8, p < 0.0001) was noted over the 2 later (May, June) vs the 2 earlier (March, April) pandemic months. There was a 1.48% stroke rate across 119,967 COVID-19 hospitalizations. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was noted in 3.3% (1,722/52,026) of all stroke admissions. CONCLUSIONS: The COVID-19 pandemic was associated with a global decline in the volume of stroke hospitalizations, IVT, and interfacility IVT transfers. Primary stroke centers and centers with higher COVID-19 inpatient volumes experienced steeper declines. Recovery of stroke hospitalization was noted in the later pandemic months. © 2021 American Academy of Neurology