In situ study of sucrose exposure, mutans streptococci in dental plaque and dental caries

The purpose of this study was to investigate the relationship among frequency of sucrose exposure, mutans streptococci levels and dental caries. Adult volunteers took part in this crossover study performed in 4 phases of 28 days each. The volunteers wore intra-oral palatal appliances containing blocks of human dental enamel and dripped 20% sucrose solution onto the dental blocks from 0 to 8 times/day. After each phase, the colony forming units (CFU) were determined in dental plaque and enamel dental caries was evaluated using cross-sectional hardness. Sucrose frequency had no statistically significant effect on mutans streptococci levels. In the enamel cross-sectional hardness tests, significant differences (p < 0.05) in relation to area of mineral loss were observed only when sucrose exposure was 8 times/day. Similar results were obtained when cross-sectional hardness was assessed at each distance from enamel surface.The purpose of this study was to investigate the relationship among frequency of sucrose exposure, mutans streptococci levels and dental caries. Adult volunteers took part in this crossover study performed in 4 phases of 28 days each. The volunteers wore intra-oral palatal appliances containing blocks of human dental enamel and dripped 20% sucrose solution onto the dental blocks from 0 to 8 times/day. After each phase, the colony forming units (CFU) were determined in dental plaque and enamel dental caries was evaluated using cross-sectional hardness. Sucrose frequency had no statistically significant effect on mutans streptococci levels. In the enamel cross-sectional hardness tests, significant differences (p < 0.05) in relation to area of mineral loss were observed only when sucrose exposure was 8 times/day. Similar results were obtained when cross-sectional hardness was assessed at each distance from enamel surface12210110

Influence Of A Cobalt-chromium Metal Framework On Surface Roughness And Knoop Hardness Of Visible Light-polymerized Acrylic Resins

Although visible light-polymerized acrylic resins have been used in removable partial dentures, it is not clear whether the presence of a metal framework could interfere with their polymerization, by possibly reflecting the light and affecting important properties, such as roughness and hardness, which would consequently increase biofilm accumulation. The aim of this study was to compare the roughness and Knoop hardness of a visible light-polymerized acrylic resin and to compare these values to those of water-bath- and microwave-polymerized resins, in the presence of a metal framework. Thirty-six specimens measuring 30.0 x 4.0 ± 0.5 mm of a microwave- (Onda Cryl), a visible light- (Triad) and a water-bath-polymerized (Clássico) (control) acrylic resins containing a cobalt-chromium metal bar were prepared. After processing, specimens were ground with 360 to 1000-grit abrasive papers in a polishing machine, followed by polishing with cloths and 1-μm diamond particle suspension. Roughness was evaluated using a profilometer (Surfcorder SE 1700) and Knoop hardness (Kg/mm 2) was assayed using a microhardness tester (Shimadzu HMV 2000) at distances of 50, 100, 200, 400 and 800 μm from the metal bar. Roughness and Knoop hardness means were submitted to two-way ANOVA and compared by Tukey and Kruskal Wallis tests at a 5% significance level Statistically significant differences were found (p&lt;0.05) for roughness and Knoop hardness, with light-polymerized resin presenting the highest values (Ra = 0.11 μm and hardness between 20.2 and 21.4 Kg/mm 2). Knoop values at different distances from the metal bar did not differ statistically (p&gt;0.05). Within the limitations of this in vitro study, it was concluded that the presence of metal did not influence roughness and hardness values of any of the tested acrylic resins.143208212Anusavice, K.J., (2003) Philip's Science of Dental Materials. 11 th Ed., p. 96. , 11 St. Louis: Elsevier ScienceBollen, C.M.L., Lambrechts, P., Quirynen, M., Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: A review of the literature (1997) Dent Mater, 13 (4), pp. 258-269Borchers, L., Tavassol, F., Tschernitschek, H., Surface quality achieved by polishing and by varnishing of temporary crown and fixed partial denture resins (1999) J Prosthet Dent, 82 (5), pp. 550-556Braun, K.O., Del Bel Cury, A.A., Cury, J.A., Use of microwave energy for processing acrylic resin near metal (1998) Braz Oral Res, 12 (2), pp. 173-180Curtis, D.A., Eggleston, T.L., Marshall, S.J., Watanabe, L.G., Shear bond strength of visible-light-cured resin relative to heat-cured resin (1989) Dental Mater, 5 (7), pp. 314-318De Clerck, J.P., Microwave polymerization of acrylic resins used in dental prosthesis (1987) J Prosthet Dent, 57 (5), pp. 650-658Khan, Z., Von Fraunhofer, J.A., Razavi, R., The staining characteristics, transverse strength, and microhardness of a visible light-cured denture base material (1987) J Prosthet Dent, 57 (3), pp. 384-386Levin, B., Sanders, J.L., Reitz, P.V., The use of microwave energy for processing acrylic resins (1989) J Prosthet Dent, 61 (3), pp. 381-383Ogle, R.E., Sorensen, S.E., Lewis, E.A., A new visible light-cured resin system applied to removable prosthodontics (1986) J Prosthet Dent, 56 (4), pp. 497-506Quirynen, M., Cml, B., Willems, G., Van Steenberghe, D., Comparison of surface characteristics of six commercially pure titanium abutments (1994) JOMI, 9 (1), pp. 71-76Reitz, P.V., Sanders, J.L., Levin, B., The curing of denture acrylic resins by microwave energy. Physical properties (1985) Quintessence Int, 6 (8), pp. 547-551Rodrigues Garcia, R.C.M., Souza Jr., J.A., Rached, R.N., Del Bel Cury, A.A., Effect of denture cleansers on the surface roughness and hardness of a microwave-cured acrylic resin and dental alloys (2004) J Prosthodont, 13 (3), pp. 1-6Rueggeberg, F.A., Goughman, W.F., Curtis Jr., J.W., Effect of light intensity and exposure duration on cure of resin composite (1994) Oper Dent, 19 (1), pp. 26-32Truong, V.T., Thomasz, F.G., Comparison of denture acrylic resins cured by boiling water and microwave energy (1988) Aust Dent J, 33 (3), pp. 201-204Ulusoy, M., Ulusoy, N., Aydin, A.K., An evaluation of polishing technique on surface roughness of acrylic rsins (1986) J Prosthetic Dent, 56 (1), pp. 107-11

Does traumatic occlusal forces lead to peri-implant bone loss? a systematic review

Observational studies have indicated that crestal bone level changes at implants are typically associated with clinical signs of inflammation, but still mechanical overload has been described as possible factor leading to hard-tissue deficiencies at implant sites without mucosal inflammation. The aim of this paper was systematically review the literature regarding the possible effect of traumatic occlusal forces on the peri-implant bone levels. Literature search was conducted using PubMed, Scielo and Lilacs, including the following terms: oral OR dental AND implant$AND (load OR overload OR excessive load OR force$ OR bruxism) AND (bone loss OR bone resorption OR implant failure$). Databases were searched for the past 10 years of publications, including: clinical human studies, either randomized or not, cohort studies, case control studies, case series and animal research. Exclusion criteria were review articles, guidelines and in vitro and in silico (finite element analysis) research, as well as retrospective studies. The PICO questions formulated was: "does traumatic occlusal forces lead to peri-implant bone loss?" The database searches as well as additional hand searching, resulted in 807 potentially relevant titles. After inclusion/exclusion criteria assessment 2 clinical and 4 animal studies were considered relevant to the topic. The included animal studies did not reveal an association between overload and peri-implant bone loss when lower overloads were applied, whereas in the presence of excessive overload it seemed to generate peri-implant bone loss, even in the absence of inflammation. The effect of traumatic occlusal forces in peri-implant bone loss is poorly reported and provides little evidence to support a cause-and-effect relationship in humans, considering the strength of a clinically relevant traumatic occlusal force33

Mechanical competence of ovariectomy-induced compromised bone after single or combined treatment with high-frequency loading and bisphosphonates

Osteoporosis leads to increased bone fragility, thus effective approaches enhancing bone strength are needed. Hence, this study investigated the effect of single or combined application of high-frequency (HF) loading through whole body vibration (WBV) and alendronate (ALN) on the mechanical competence of ovariectomy-induced osteoporotic bone. Thirty-four female Wistar rats were ovariectomized (OVX) or sham-operated (shOVX) and divided into five groups: shOVX, OVX-shWBV, OVX-WBV, ALN-shWBV and ALN-WBV. (Sham)WBV loading was applied for 10 min/day (130 to 150 Hz at 0.3g) for 14 days and ALN at 2 mg/kg/dose was administered 3x/week. Finite element analysis based on micro-CT was employed to assess bone biomechanical properties, relative to bone micro-structural parameters. HF loading application to OVX resulted in an enlarged cortex, but it was not able to improve the biomechanical properties. ALN prevented trabecular bone deterioration and increased bone stiffness and bone strength of OVX bone. Finally, the combination of ALN with HF resulted in an increased cortical thickness in OVX rats when compared to single treatments. Compared to HF loading, ALN treatment is preferred for improving the compromised mechanical competence of OVX bone. In addition, the association of ALN with HF loading results in an additive effect on the cortical thickness

Candida albicans increases dentine demineralization provoked by streptococcus mutans biofilm

Streptococcus mutans are considered the most cariogenic bacteria, but it has been suggested that Candida albicans could increase their cariogenicity. However, the effect of this dual-species microorganisms’ combination on dentine caries has not been experimentally evaluated. Biofilms of C. albicans, S. mutans and C. albicans + S. mutans (n = 12/biofilm) were grown in ultra-filtered tryptone yeast extract broth culture medium for 96 h on root dentine slabs of known surface hardness and exposed 8 times per day for 3 min to 10% sucrose. The medium was changed 2 times per day (after the 8 cariogenic challenges and after the overnight period of famine), and aliquots were analyzed to determinate the pH (indicator of biofilm acidogenicity). After 96 h, the biofilms were collected to determine the wet weight, colony-forming units, and the amounts of extracellular polysaccharides (soluble and insoluble). Dentine demineralization was assessed by surface hardness loss (% SHL). The architecture of the biofilms was analyzed by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). Data were analyzed by ANOVA followed by Tukey’s test (α = 0.05). The dual-species C. albicans + S. mutans biofilm provoked higher % SHL on dentine (p < 0.05) than the S. mutans and C. albicans biofilm. This was supported by the results of biofilm acidogenicity and the amounts of soluble (6.4 ± 2.14 vs. 4.0 ± 0.94 and 1.9 ± 0.97, respectively) and insoluble extracellular polysaccharides (24.9 ± 9.22 vs. 18.9 ± 5.92 and 0.7 ± 0.48, respectively) (p < 0.05). The C. albicans biofilm alone presented low cariogenicity. The images by CLSM and TEM, respectively, suggest that the C. albicans + S. mutans biofilm is more voluminous than the S. mutans biofilm, and S. mutans cells interact with C. albicans throughout polysaccharides from the biofilm matrix. These findings show that C. albicans enhances the cariogenic potential of the S. mutans biofilm, increasing dentine demineralization533322331CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ165331/2013-

The Role Of Dentistry In The Interdisciplinary Team: Contributing To Comprehensive Health Care For The Elderly [o Papel Da Odontologia Na Equipe Interdisciplinar: Contribuindo Para A Atenção Integral Ao Idoso.]

This literature review focuses on dentistry's role in comprehensive health care for the elderly. The authors discuss the need for an interdisciplinary approach. They begin by analyzing the current situation in geriatric dentistry and related problems in Brazil, relating primarily to the lack of specific studies and human resources with training in geriatrics and gerontology. The authors emphasize interactions between dentistry and other health professions for health promotion, specific prevention, and rehabilitation of elderly patients, with special attention to the importance of communication and information exchange.1641099110

Effect of a calcium carbonate-based dentifrice on in situ enamel remineralization

This crossover study evaluated the effect of calcium carbonate (CaCO(3))-based monofluorophosphate dentifrice on enamel remineralization. Ten volunteers wore palatal appliances containing four enamel blocks with caries-like lesions, two of them covered by a test plaque of mutans streptococci. The following treatments were evaluated: a negative control, a silica-based or a CaCO(3)-based dentifrice, and the percentage of enamel surface microhardness recovery was determined. The CaCO(3)-based dentifrice was more effective than the negative control on the enhancement of enamel remineralization, either in the presence or absence of test plaque. This efficacy was also shown by the silica-based dentifrice but only in the presence of test plaque.This crossover study evaluated the effect of calcium carbonate (CaCO 3 )-based monofl uorophosphate dentifrice on enamel remineralization. Ten volunteers wore palatal appliances containing four enamel blocks with carieslike lesions, two of them covered by a test plaque of mutans streptococci. The following treatments were evaluated: a negative control, a silica-based or a CaCO 3 -based dentifrice, and the percentage of enamel surface microhardness recovery was determined. The CaCO 3 -based dentifrice was more effective than the negative control on the enhancement of enamel remineralization, either in the presence or absence of test plaque. This effi cacy was also shown by the silica-based dentifrice but only in the presence of test plaque39325525