Questionário De Percepção Dos Pais - Q.P.P.

Based on the relevant psychological and sociologicalliterature, a 43 item scale (QPP) was built to assess subjects'perceptions of their own parents. A factorial analysis of the answers of574 high-school and college students revealed four factors in thepaternal figure: Friendly companionship, punitive discipline, lax discipline,and centration in the child. Four factors also appeared in thematernal figure: Friendly intimacy, overprotection, lax discipline, andpunishment. Two second-order factors appeared in both parentalfigures: Love and discipline. Ali factors showsatisfactory reliability. Theinstrument as well as the form of its practical use are presented.Com base na literatura psicológica e sociológica pertinente, foi construído um instrumento de 43 itens (QPP) para avaliar a percepção que os filhos possuem de seus pais. A validação fatorial foi efetuada com uma amostra de 574 estudantes de 2? grau e superior. Quatro fatores apareceram na figura do pai: companheiro amigo, disciplina punitiva, disciplina lassa, centralização no filho; quatro fatores surgiram também na figura da mãe: intimidade amiga, superproteção, controle lasso, punição. Esses quatro fatores em ambas as figuras parentais se agrupam em dois fatores de segunda ordem: amore disciplina. Todos eles possuem índices satisfatórios de precisão. São apresentados igualmente o instrumento e o seu modo de utilização

Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine

The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries

Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine

The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries

Synthesis of LaNiO3 perovskite by the modified proteic gel method and study of catalytic properties in the syngas production

Este trabalho descreve um estudo sobre a síntese da perovsquita LaNiO3 via método do gel proteico, variando quantidade de colágeno e sobre a atividade catalítica de LaNiO3 e LaNiO3/Al2O3 na produção de gás de síntese (CO + H2). Os padrões de difração de raios X revelaram a formação da estrutura perovsquita em todas as amostras preparadas pelo método de síntese do gel protéico, variando a quantidade de colágeno e após calcinações a 700 ºC por 2 h. O catalisador LaNiO3/Al2O3 preparado pelo método de impregnação mostrou picos de difração nos difratogramas atribuídos a LaNiO3 e ao suporte (Al2O3). Este catalisador apresentou: área superficial específica de 46,1 m2 g-1, dois picos de redução no perfil de redução à temperatura programada (RTP) e 46% de conversão do metano (através da oxidação parcial do metano utilizando oxigênio) depois de 18 h de reação. _________________________________________________________________________________________ ABSTRACT: This work describes a study on the synthesis of LaNiO3 perovskites via the modified proteic gel method, varying collagen content and on the catalytic activity of LaNiO3 and LaNiO3/Al2O3 in the syngas (CO + H2) production. X-ray diffraction patterns revealed the formation of perovskite structure in all samples prepared by proteic gel synthesis method, varying collagen content and after calcinations at 700 ºC for 2 h. LaNiO3/Al2O3 catalyst prepared by the impregnation method showed diffraction peaks due to the perovskite structure and to the support (Al2O3). This catalyst presented: specific surface of 46.1 m2 g-1, two reduction peaks in the temperature programmed reduction (TPR) profile and 46% of methane conversion (by the partial oxidation of methane using oxygen) after 18 h of reaction

Bioassay-Guided Evaluation of Antinociceptive Effect of N-Salicyloyltryptamine: A Behavioral and Electrophysiological Approach

We investigated the antinociceptive and nerve excitability effects of the N-salicyloyltryptamine (NST) NST-treated mice exhibited a significant decrease in the number of writhes when 100 and 200 mg/kg (i.p.) were administered (i.p.). This effect was not antagonized by naloxone (1.5 mg/kg, i.p.). NST inhibited the licking response of the injected paw when 100 and 200 mg/kg were administered (i.p.) to mice in the first and second phases of the formalin test. Because the antinociceptive effects could be associated with neuronal excitability inhibition, we performed the single sucrose gap technique and showed that NST (3.57 mM) significantly reduced (29.2%) amplitude of the compound action potential (CAP) suggesting a sodium channel effect induced by NST. Our results demonstrated an antinociceptive activity of the NST that could be, at least in part, associated to the reduction of the action potential amplitude. NST might represent an important tool for pain management

Prevalence and predictors of coronary artery disease in patients with a calcium score of zero

The absence of coronary calcification is associated with an excellent prognosis. However, a calcium score of zero does not exclude the presence of coronary artery disease (CAD) or the possibility of future cardiovascular events. Our aim was to study the prevalence and predictors of coronary artery disease in patients with a calcium score of zero. Prospective registry consisted of 3,012 consecutive patients that underwent cardiac CT (dual source CT). Stable patients referred for evaluation of possible CAD that had a calcium score of zero (n = 864) were selected for this analysis. The variables that were statistically significant were included in a multivariable logistic regression model. From 864 patients with a calcium score of zero, 107 (12.4 %) had coronary plaques on the contrast CT (10.8 %, n = 93 with nonobstructive CAD and 1.6 %, n = 14 with obstructive CAD). By logistic regression analysis, the independent predictors of CAD in this population were age >55 years [odds ratio (OR) 1.63 (1.05-2.52)], hypertension [OR 1.64 (1.05-2.56)] and dyslipidemia [OR 1.54 (1.00-2.36)]. In the presence of these 3 variables, the probability of having coronary plaques was 21 %. The absence of coronary artery calcification does not exclude the presence of coron

Acetylated cashew gum-based nanoparticles for the incorporation of alkaloid epiisopiloturine

The natural alkaloid epiisopiloturine has recently become the focus of study for various medicinal properties, particularly for its anti-inflammatory and antischistosomal effect. The incorporation of active molecules in natural polymeric matrices has garnered increasing interest during recent decades. A new derivative of cashew gum successfully obtained by gum acetylation has shown great potential as a carrier in controlled drug release systems. In this work, epiisopiloturine was encapsulated in acetylated cashew gum nanoparticles in order to increase solubility and allow slow release, whereas the morphology results were supported by computer simulations. The particles were produced under a variety of conditions, and thoroughly characterized using light scattering and microscopic techniques. The particles were spherical and highly stable in solution, and showed drug incorporation at high levels, up to 55% efficiency. Using a dialysis-based in vitro assay, these particles were shown to release the drug via a Fickian diffusion mechanism, leading to gradual drug release over approximately 6 h. These nanoparticles show potential for the use as drug delivery system, while studies on their potential anti-inflammatory action, as well as toxicity and efficacy assays would need to be performed in the future to confirm their suitability as drug delivery candidates.This work was conducted in partnership with the Polymer Laboratory of the Federal University of Ceará for polymer modification. The authors thanks Foundation for Science and Technology (FCT) for the fellowships SFRH/BD/97995/2013 (AP) and SFRH/BD/95983/2013 (MPA), in the context of the POCH program. The work at UCIBIO/REQUIMTE was supported by FCT through project UID/MULTI/04378/2013 – POCI/01/0145/FEDER/007728 with financial support from FCT/MCTES through national funds and co-financed by FEDER, under the Partnership Agreement PT2020. The work at REQUIMTE/LAQV received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013. The computational time was provided by GRID-Unesp, SICC/IFSP and CENAPAD/SP. The authors also acknowledge CNPq and CAPES for a scholarship and financial aid.info:eu-repo/semantics/publishedVersio

Quaternized cashew gum: An anti-staphylococcal and biocompatible cationic polymer for biotechnological applications

Chemical modifications to cashew gum (CG) structure have been previously reported to obtain new physicochemical characteristics, however until now there were no reports of modifications by introduction of new functional groups to add cationic character. This study presents a quaternization route for CG using a quaternary ammonium reagent. The chemical features of the quaternized cashew gum derivatives (QCG) were analyzed by: FTIR, elemental analysis, degree of substitution, Zeta potential, 1H NMR and 1H-13C correlation (HSQC). QCG were evaluated for their anti-staphylococcal activity by determining minimum inhibitory and bactericidal concentrations against pathogenic Staphylococcus spp. and by imaging using atomic force microscopy. Moreover, the mammalian cell biocompatibility were also assessed through hemolytic and cell toxicity assays. QCG presented promising antimicrobial activity against methicillin-resistant S. aureus and biocompatibility on tested cells. These results show that QCG could be a promising tool in the development of biomaterials with an anti-septic action.info:eu-repo/semantics/publishedVersio