CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

Open Access Journal; Published online: 31 Jan 2019Presence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated

Active Region Moss: Doppler Shifts from Hinode/EIS Observations

Studying the Doppler shifts and the temperature dependence of Doppler shifts in moss regions can help us understand the heating processes in the core of the active regions. In this paper we have used an active region observation recorded by the Extreme-ultraviolet Imaging Spectrometer (EIS) onboard Hinode on 12-Dec-2007 to measure the Doppler shifts in the moss regions. We have distinguished the moss regions from the rest of the active region by defining a low density cut-off as derived by Tripathi et al. (2010). We have carried out a very careful analysis of the EIS wavelength calibration based on the method described in Young et al. (2012). For spectral lines having maximum sensitivity between log T = 5.85 and log T = 6.25 K, we find that the velocity distribution peaks at around 0 km/s with an estimated error of 4-5 km/s. The width of the distribution decreases with temperature. The mean of the distribution shows a blue shift which increases with increasing temperature and the distribution also shows asymmetries towards blue-shift. Comparing these results with observables predicted from different coronal heating models, we find that these results are consistent with both steady and impulsive heating scenarios. However, the fact that there are a significant number of pixels showing velocity amplitudes that exceed the uncertainty of 5 km s$^{-1}$ is suggestive of impulsive heating. Clearly, further observational constraints are needed to distinguish between these two heating scenarios.Comment: 21 pages (single column), 7 figures, Accepted for Publication in The Astrophysical Journa

Modified 2D Proca Theory: Revisited Under BRST and (Anti-)Chiral Superfield Formalisms

Within the framework of Becchi-Rouet-Stora-Tyutin (BRST) approach, we discuss mainly the fermionic (i.e. off-shell nilpotent) (anti-)BRST, (anti-)co-BRST and some discrete dual-symmetries of the appropriate Lagrangian densities for a two (1+1)-dimensional (2D) modified Proca (i.e. a massive Abelian 1-form) theory without any interaction with matter fields. One of the novel observations of our present investigation is the existence of some kinds of restrictions in the case of our present St\"{u}ckelberg-modified version of the 2D Proca theory which is not like the standard Curci-Ferrari (CF)-condition of a non-Abelian 1-form gauge theory. Some kinds of similarities and a few differences between them have been pointed out in our present investigation. To establish the sanctity of the above off-shell nilpotent (anti-)BRST and (anti-)co-BRST symmetries, we derive them by using our newly proposed (anti-)chiral superfield formalism where a few specific and appropriate sets of invariant quantities play a decisive role. We express the (anti-)BRST and (anti-)co-BRST conserved charges in terms of the superfields that are obtained after the applications of (anti-)BRST and (anti-)co-BRST invariant restrictions and prove their off-shell nilpotency and absolute anticommutativity properties, too. Finally, we make some comments on (i) the novelty of our restrictions/obstructions, and (ii) the physics behind the negative kinetic term associated with the pseudo-scalar field of our present theory.Comment: LaTeX file, 58 pages, Journal reference give

Nuclear magnetic resonance probes for the Kondo scenario for the 0.7 feature in semiconductor quantum point contact devices

We propose a probe based on nuclear relaxation and Knight shift measurements for the Kondo scenario for the "0.7 feature" in semiconductor quantum point contact (QPC) devices. We show that the presence of a bound electron in the QPC would lead to a much higher rate of nuclear relaxation compared to nuclear relaxation through exchange of spin with conduction electrons. Furthermore, we show that the temperature dependence of this nuclear relaxation is very non-monotonic as opposed to the linear-T relaxation from coupling with conduction electrons. We present a qualitative analysis for the additional relaxation due to nuclear spin diffusion (NSD) and study the extent to which NSD affects the range of validity of our method. The conclusion is that nuclear relaxation, in combination with Knight shift measurements, can be used to verify whether the 0.7 feature is indeed due to the presence of a bound electron in the QPC.Comment: Published version. Appears in a Special Section on the 0.7 Feature and Interactions in One-Dimensional Systems. 16 page

Efficiency bounds for estimating linear functionals of nonparametric regression models with endogenous regressors

The main objective of this paper is to derive the efficiency bounds for estimating certain linear functionals of an unknown structural function when the latter is not itself a conditional expectation.