Study of fertility and cytogenetic variability in androgenic plants (R0 and R1) of alloplasmic introgression lines of common wheat

Anther culture is one of the methods to obtain DH lines of wheat. A limitation of this method can be cytogenetic instability in plants R0, leading to a decrease in fertility or sterility. In this study, we have investigated the fertility of R0, the fertility and cytogenetic variability of R1 in alloplasmatic introgression lines of common wheat in order to develop a cytogenetically stable DH lines with introgressions from different species. Lines 311/134, 311/FL, 311/IR with the cytoplasm from H. vulgare were studied. 311/134 carries the wheat-rye 1RS.1BL and wheatwheatgrass 7DL-7Ai translocations; 311/FL has the 1RS.1BL translocation and probably introgressions from A. glaucum; and 311/IR has the wheat-rye 1RS.1BL and wheat-Ae. speltoides T2B/2S#2 translocations. Green seedlings developed in anther culture for all lines. Differences between the lines in the ability for androgenesis and in the level of fertility in R0 and R1 have been revealed. Depressed androgenesis, low fertility and high aneuploidy were observed in 311/IR. It has been proposed that the reason for this is cytogenetic instability in gametes, which is caused by Gc genes located on T2B/2S#2. 63.3 % of 311/134 and 311/FL R1 plants that were grown from low seed-set R0 plants were aneuploids. Fertile R0 regenerant plants were identified that segregated in R1 for fertility and chromosome numbers. It has been demonstrated that DH lines are best developed from highfertility R1 plants with 2n = 42 irrespective of fertility in R0

Мультидисциплинарный подход к диагностике неспецифической интерстициальной пневмонии (клиническое наблюдение)

Nonspecific interstitial pneumonia (NSIP) is one of idiopathic interstitial pneumonias. NSIP is characterized by non-specific clinical, radiologic, and morphological patterns; thereby, multidisciplinary approach is most reasonable to diagnose NSIP. A clinical case of NSIP is reported in the article. The case demonstrated that the multi-slice computed tomography of the lungs is more valuable to diagnose NSIP compared to the lung tissue biopsy, as typical morphological signs are absent. The multidisciplinary approach was also very useful in this case.Одной из форм идиопатической интерстициальной пневмонии является неспецифическая интерстициальная пневмония (НСИП), особенность которой заключается в неспецифичности клинической, рентгенологической и морфологической картины. По данным приведенного клинического наблюдения показано, что в отсутствие патогномоничных гистологических маркеров НСИП существенно понижается ценность проведения биопсии легких как диагностического метода в этой ситуации и в большей степени определяется важность других инструментальных методов, в первую очередь мультиспиральной компьютерной томографии. По данным представленного клинического наблюдения продемонстрирована эффективность мультидисциплинарного подхода с привлечением различных специалистов при диагностике НСИП

Studying sintactic features of advertising texts

The article describes the results of observation and a survey to assess the perception of various headings of advertising text

FEATURES OF ANDROGENESIS IN ANTHER CULTURES OF VARIETIES AND A PROMISING ACCESSION OF SPRING COMMON WHEAT BRED IN WEST SIBERIA DIFFERING IN THE PRESENCE OR ABSENCE OF WHEAT-ALIEN TRANSLOCATIONS

Androgenesis has been studied in anther cultures of eight cultivars and one promising accession of spring common wheat raised in West Siberia (Siberian Research Institute of Agriculture, Omsk, Russia). The varieties are close in origin but vary in the presence or absence of wheat-alien translocations (wheat–rye 1RS.1BL and wheat–couch grass 7DL-7Ai). The promising accession L-311/00-22 bears the 1RS.1BL translocation and the cytoplasm of cultivated barley Hordeum vulgare L. The main task of the study is to assess the possibility of obtaining dihaploid lines in the genotypes examined bearing wheat-alien translocations. It has been found that different accessions respond differently to anther culture conditions depending on the concentration of 2,4-D in the initial medium. Accession L-311/00-22 is best for androgenesis experiments and raise of dihaploid lines. The dependence of the effect of the genotypic environment of wheat on the effect of wheat-alien translocation on androgenesis features, is discussed

Органический углерод и нефтяные углеводороды в донных отложениях Севастопольской бухты (Черное море)

Сделано обобщение предшествующих и полученных новых данных о межгодовых колебаниях в распределении органического углерода, хлороформных битумоидов и нефтяных углеводородов в донных отложениях Севастопольской бухты. За последние годы отмечается явное обогащение донных осадков органическим веществом. Наличие локальных районов, отличающихся высокими концентрациями органического углерода в осадках, обусловлено различными условиями осадкообразования и неодинаковой степенью антропогенной нагрузки на различные участки бухты. Как следствие, такие осадки имеют восстановительные условия, способствующие накоплению битумоидов и нефтяных углеводородов.The paper summarizes data available from the literature and recently obtained about interannual variations of the content of organic carbon, chloroform bitumenoids and oil hydrocarbons found in bottom sediments of Sevastopol Bay (Black Sea). For the recent years the bottom sediment has been receiving a rich inflow of organic matter. Localities where organic carbon content is especially high appeared owing to special sedimentation conditions and unevenly distributed anthropogenic load on the bay's water area. As the result, such sediments have reductive properties which foster accumulation of bitumenoids and oil hydrocarbons

ANDROGENESIS ABILITY IN COMMON WHEAT EUPLASMIC LINES AND ALLOPLASMIC RECOMBINANT LINES (H. VULGARE)-T. AESTIVUM POSSESSING 1RS.1BL AND 7DL-7AI TRANSLOCATIONS AND PRODUCTION OF DOUBLE HAPLOID LINES

Androgenesis ability was studied in anther cultures of euplasmic lines of common wheat and alloplasmic recombinant lines (H. vulgare)-T. aestivum with 1RS.1BL and 7DL-7Ai translocations. The ability to produce androgenic structures and plantlet regeneration are suppressed in lines carrying both translocations. Alloplasmic recombinant lines (H. vulgare)-T. aestivum with 1RS.1BL and 7DL-7Ai translocations, as well as alloplasmic lines with 1RS.1BL translocation, are characterized by increased ability to create androgenic structures, including polyembryos, and plantlet regeneration as compared to euplasmic lines. The inducing reciprocal influence of barley cytoplasm and rye chromosome 1RS on the androgenesis ability of lines (H. vulgare)-T. aestivum with 1RS.1BL and 7DL-7Ai translocations is discussed. Double haploid lines were developed from androgenic plants with spontaneously doubled chromosome numbers and restored fertility. Of the lines carrying the translocations, the most promising with regard to the manifestation of commercially valuable traits and resistance to diseases were selected in order to utilize them in breeding programs

CRYPTOGENIC STROKE IN GENERAL PRACTICE

This article is a clinical case description of a young female (37 yrs) with ischemic stroke of unknown origin. Neurologic and cardiovascular investigations failed to find out any potential reason for stroke in this case. Genetic test on prothrombotic mutations helped to identify several genetic abnormalities associated with high thrombotic risk that could lead this patient to ischemic event on the background of dysplasia of one of the brain arteries. The authours suggest an algorithmic approach to diagnose young patients (< 50 yrs) with stroke of unknown origin