Effect of biologically active additives based on the homogenate of drone larvae on microcirculation and metabolism in Nordic skiers

Objective: to study the effect of biologically active additives (BAA) based on the homogenate of drone larvae on microcirculation and metabolism in Nordic skiers. Materials and methods: 58 male skiers at the age of 18-20 years ranging from 1 category to CMS were divided into two groups: the main group (30 athletes) and the comparison group (28 athletes). The skiers of the main group used the homogenate of drone larvae daily at a dose of 10 mg/kg of body weight during 21 days. Athletes from the comparison group received potato starch (placebo). The study of the microcirculation system was performed with a laser analyzer of the capillary blood flow «LAKK-M» before the BAA administration, at the 1st, 10th and 21st days of the study and 30 days after the end of the BAA administration. Results: A significant increase in perfusion was observed in the experimental group (121%,

How to Search for Doubly Charmed Baryons and Tetraquarks

Possible experimental searches of doubly charmed baryons and tetraquarks at fixed target experiments with high energy hadron beams and a high intensity spectrometer are considered here. The baryons considered are: $\Xi_{cc}^{+}$ (ccd), $\Xi_{cc}^{++}$ (ccu), and $\Omega_{cc}^{+}$ (ccs); and the tetraquark is T ($cc\bar{u}\bar{d}$). Estimates are given of masses, lifetimes, internal structure, production cross sections, decay modes, branching ratios, and yields. Experimental requirements are given for optimizing the signal and minimizing the backgrounds. The discussion is in the spirit of an experimental and theoretical review, as part of the planning for a CHarm Experiment with Omni-Purpose Setup (CHEOPS) at CERN. The CHEOPS objective is to achieve a state-of-the-art very charming experiment, in the spirit of the aims of the recent CHARM2000 workshop.Comment: 18 pages text (latex), 16 March 1995, presented at "Physics with Hadron Beams with a High Intensity Spectrometer", revised 10 May for more complete bibliography and appropriate references to S. Paul et al., Letter of Intent, CHEOPS

Genetic landscape in Russian patients with familial left ventricular noncompaction

BackgroundLeft ventricular noncompaction (LVNC) cardiomyopathy is a disorder that can be complicated by heart failure, arrhythmias, thromboembolism, and sudden cardiac death. The aim of this study is to clarify the genetic landscape of LVNC in a large cohort of well-phenotyped Russian patients with LVNC, including 48 families (n=214).MethodsAll index patients underwent clinical examination and genetic analysis, as well as family members who agreed to participate in the clinical study and/or in the genetic testing. The genetic testing included next generation sequencing and genetic classification according to ACMG guidelines.ResultsA total of 55 alleles of 54 pathogenic and likely pathogenic variants in 24 genes were identified, with the largest number in the MYH7 and TTN genes. A significant proportion of variants −8 of 54 (14.8%) −have not been described earlier in other populations and may be specific to LVNC patients in Russia. In LVNC patients, the presence of each subsequent variant is associated with increased odds of having more severe LVNC subtypes than isolated LVNC with preserved ejection fraction. The corresponding odds ratio is 2.77 (1.37 −7.37; p <0.001) per variant after adjustment for sex, age, and family.ConclusionOverall, the genetic analysis of LVNC patients, accompanied by cardiomyopathy-related family history analysis, resulted in a high diagnostic yield of 89.6%. These results suggest that genetic screening should be applied to the diagnosis and prognosis of LVNC patients

CHEMOKINE SECRETION PATTERNS IN MONONUCLEAR AND DENDRITIC CELLS: ROLE OF HISTAMINE TYPE H3/H4 RECEPTORS

The study presents results concerning effects of H3/H4 histamine receptors antagonist (Ciproxifan) upon in vitro chemokine secretion by dendritic cells (DC) and peripheral blood mononuclear cells (PBMC). We have been shown that in vitro inhibition of type H3/H4 histamine receptor affects in different ways production of major chemokines by PBMC and DC, including eotaxin, RANTES, MCP-1, MIP-1α, MIP-1β, GRO-α, and IL-8. E.g., RANTES and GRO-α secretion in DC cultures was reduced, respectively, by 20% and 40%. Meanwhile, MIP-1β secretion was virtually unchanged, whereas MCP-1 secretion exhibited a four-fold increase. In PBMC cultures, the H3/H4 receptor antagonist induced a significant increase in eotaxin, RANTES, MIP-1α, MIP-1β, GRO-α, and IL-8 secretion (p < 0.05)

Influence of kyphosis correction in thoracolumber spine on physical activity of children with infantile cerebral palsy

Objective - to determinate the influence of kyphosis correction in thoracic spine on the ability to have vertical balance of the body in patients with cerebral palsy. Material and methods. The authors have performed a comprehensive survey of 17 patients with cerebral palsy aged from 6 to 17 years with the generated non-fixed "habitual" thoracolumbar kyphosis associated with joint contractures of the lower extremities. The analysis of the radiological data was carried out to determine the dependence of severity of kyphosis on the position of the body. The state of the vertical stability of children before and after thoracolumbar orthosis with body corset were evaluated by means of computed stabilometrics. Results. Radiological findings in all children have shown a normal correlation of changes in the degree of kyphosis - its reduction if there was an exclusion of the vertical load on the spine, as well as the trunk orthosis. In this case correction of sagittal profile of the spine occurred mainly due to the lumbar area. This feature of spinal biomechanics in patients with infantile cerebral palsy unpredictably reflected on the motor activity of patients. After correction of kyphosis with corset only 29.4% of the children surveyed with stabilometrics demonstrated the improvement in the function of maintaining the vertical position of the body. In 70.6% of patients the correction of sagittal profile of the spine has led to a deterioration of the vertical balance of the body. Negative dynamics of test research in this group presupposes the exhaustion of children’s adaptive capacity, which indicates the risk of developing abnormal compensatory reactions because of corset bracing. Conclusion. The advanced integrated observation of the patients with cerebral palsy can improve the prediction of outcomes in planning of the correction of kyphosis in thoracolumbar spine

Evaluation of nonlinear dynamics of the cell structure damage as a promising method for personalized cancer diagnostics

Background: Modulation interference microscopy is one of the promising technologies for early personalized cancer diagnostics, while it gives the possibility to obtain real-time images of living functioning cells at a nanometer resolution, as well as to assess spatio-temporal changes of their intracellular structures.Aim: To identify the markers of malignant transformation of cells based on a multiple scale spatio-temporal of the data obtained by lase interferometry during in situ registration of changes in intracellular structures.Materials and methods: The study was performed with the breast adenocarcinoma MCF-7 cell line and human kidney epithelial cells HEK 293 provided by the N.N. Blokhin National Medical Research Centre of Oncology (Moscow, Russia). Changes in fluctuation of the living cell phase thickness were measured with a laser modulation interference microscope MIM-340 (PA UOMZ, Russia). The multifractal detrended fluctuation analysis (MF-DFA) was used to assess the results obtained.Results: By comparison of the scaling exponential spectra and corresponding spectra of the singular fluctuations in the minimal and maximal diameters of the phase pictures of HEK 293 cells and MCF-7 cells, it was shown that the cancer cell dynamics has definite monofractal properties: a linear dependency of the scaling exponents τ from the actual q parameter and “dotted” singularity spectrum (the spectral width does not exceed 0.1). The dynamics of healthy cell demonstrated multifractal properties, such as a non-linear dependency τ(q) and a bell shaped singularity spectrum (the spectral width over 0.1).Conclusion: We have proposed a new approach to assess the intracellular structural damage during oncogenesis. The signs of monofractality are objective criteria of malignization. The results obtained seems promising as early diagnostic markers of cancer transformation (damage) of cells and determination of tumor cell sensitivity / resistance to anti-tumor agents in an individual patient

Клінічні особливості міксоми лівого передсердя в поєднанні з активним туберкульозом легень

The symptoms and syndromology of myxomas with clinical features of a rare comorbidity – lung tuberculosis and left atrial myxoma in a 69-old year woman, are described in the article. The description includes the clinical history, transesophageal echocardiography findings, pathomorphological characteristics of myxoma, also photoillustrations of tumor’s macro- and microstructures. The analysis reflects the troubles in differential diagnosis of the clinical case due to non-specific symptoms of both diseases. The discussion provides the information about the possible pathophysiological link between myxoma and tuberculosis and the role of interleukin6 inthis process.В статье описаны симптоматика и синдромология миксом, а также клинические проявления редкого сочетания двух заболеваний – туберкулёза лёгких и миксомы левого предсердия у 69-летней женщины. Приводятся результаты клинического обследования пациентки, чреспищеводной эхокардиографии, патоморфологического исследования миксомы и фотоиллюстрации макро-, микроструктуры опухоли. Анализируется сложность дифференциальной диагностики в представленном наблюдении ввиду неспецифичности симптоматики обоих заболеваний. Обсуждается вопрос возможной патогенетической связи между миксомой и туберкулёзной инфекцией, а также роль интерлейкина-6 в этом процессе.У статті описані симптоматика та синдромологія міксом, а також клінічні прояви рідкого поєднання двох хвороб – туберкульозу легень і міксоми лівого передсердя у 69-річної жінки. Наведені результати клінічного обстеження пацієнтки, черезстравохідної ехокардіографії, патоморфологічного дослідження міксоми та фотоілюстрації макро-, мікроструктури пухлини. Аналізується складність диференціальної діагностики в цьому випадку через неспецифічність симптоматики обох хвороб. Обговорюється питання можливого патогенетичного зв’язку між міксомою та туберкульозною інфекцією, крім того, роль інтерлейкіна-6 у цьому процесі

Сlinical features of left atrial myxoma in comorbidity with active lung tuberculosis

The symptoms and syndromology of myxomas with clinical features of a rare comorbidity – lung tuberculosis and left atrial myxoma in a 69-old year woman, are described in the article. The description includes the clinical history, transesophageal echocardiography findings, pathomorphological characteristics of myxoma, also photoillustrations of tumor’s macro- and microstructures. The analysis reflects the troubles in differential diagnosis of the clinical case due to non-specific symptoms of both diseases. The discussion provides the information about the possible pathophysiological link between myxoma and tuberculosis and the role of interleukin6 inthis process

Effects of Tyrosine and Tryptophan in Rats with Diet-Induced Obesity

Amino acids tyrosine (Tyr) and tryptophan (Trp) play a significant role in the regulation of energy metabolism, locomotor activity, and eating behavior. We studied the possibility of modulating these processes in obesity by increasing the pool of Tyr and Trp in the experimental diet. As a model of obesity, we used Wistar rats fed a diet with an excess specific energy value (HFCD) for 64 days. Trp led to a normalization of the rats’ body weight almost to the control level, but increased anxiety-like behavior and decreased long-term memory. The consumption of amino acids resulted in increased grip strength and impairment of short-term memory. The locomotor activity of animals decreased with age as a result of Tyr consumption, while Trp, on the contrary, prevented this. The Tyr supplementation led to the normalization of triglycerides and LDL. In the spleen cell lysates, amino acids suppressed the production of proinflammatory cytokines. The liver tissue morphology showed that the consumption of Tyr noticeably weakened the signs of fatty degeneration. The addition of Trp, on the contrary, led to an unfavorable effect, consisting of the appearance of a high number of large rounded fatty vacuoles. The data obtained indicate a more pronounced anti-inflammatory effect of Tyr as compared to Trp