Electrostatically Tunable Meta-Atoms Integrated With In Situ Fabricated MEMS Cantilever Beam Arrays

Two concentric split ring resonators (SRRs) or meta-atoms designed to have a resonant frequency of 14 GHz are integrated with microelectromechanical systems cantilever arrays to enable electrostatic tuning of the resonant frequency. The entire structure was fabricated monolithically to improve scalability and minimize losses from externally wire-bonded components. A cantilever array was fabricated in the gap of both the inner and outer SRRs and consisted of five evenly spaced beams with lengths ranging from 300 to 400 μm. The cantilevers pulled in between 15 and 24 V depending on the beam geometry. Each pulled-in beam increased the SRR gap capacitance resulting in an overall 1-GHz shift of the measured meta-atom resonant frequency

Induced pluripotent stem cell-based therapy for age-related macular degeneration

Introduction: In age-related macular degeneration (AMD), stem cells could possibly replace or regenerate disrupted pathologic retinal pigment epithelium (RPE), and produce supportive growth factors and cytokines such as brain-derived neurotrophic factor. Induced pluripotent stem cells (iPSCs)-derived RPE was first subretinally transplanted in a neovascular AMD patient in 2014. Areas covered: Induced PSCs are derived from the introduction of transcription factors to adult cells under specific cell culture conditions, followed by differentiation into RPE cells. Induced PSC-derived RPE cells exhibit ion transport, membrane potential, polarized VEGF secretion and gene expression that is similar to native RPE. Despite having similar in vitro function, morphology, immunostaining and microscopic analysis, it remains to be seen if iPSC-derived RPE can replicate the myriad of in vivo functions, including immunomodulatory effects, of native RPE cells. Historically, adjuvant RPE transplantation during CNV resections were technically difficult and complicated by immune rejection. Autologous iPSCs are hypothesized to reduce the risk of immune rejection, but their production is time-consuming and expensive. Alternatively, allogenic transplantation using human leukocyte antigen (HLA)-matched iPSCs, similar to HLA-matched organ transplantation, is currently being investigated. Expert opinion: Challenges to successful transplantation with iPSCs include surgical technique, a pathologic subretinal microenvironment, possible immune rejection, and complications of immunosuppression

SRRs Embedded with MEMS Cantilevers to Enable Electrostatic Tuning of the Resonant Frequency

A microelectromechanical systems (MEMS) cantilever array was monolithically fabricated in the gap region of a split ring resonator (SRR) to enable electrostatic tuning of the resonant frequency. The design consisted of two concentric SRRs each with a set of cantilevers extending across the split region. The cantilever array consisted of five beams that varied in length from 300 to 400 μm, with each beam adding about 2 pF to the capacitance as it actuated. The entire structure was fabricated monolithically to reduce its size and minimize losses from externally wire bonded components. The beams actuate one at a time, longest to shortest with an applied voltage ranging from 30–60 V. The MEMS embedded SRRs displayed dual resonant frequencies at 7.3 and 14.2 GHz or 8.4 and 13.5 GHz depending on the design details. As the beams on the inner SRR actuated the 14.2 GHz resonance displayed tuning, while the cantilevers on the outer SRR tuned the 8.4 GHz resonance. The 14.2 GHz resonant frequency shifts 1.6 GHz to 12.6 GHz as all the cantilevers pulled-in. Only the first two beams on the outer cantilever array pulled-in, tuning the resonant frequency 0.4 GHz from 8.4 to 8.0 GHz

Background-Limited Imaging in the Near-Infrared with Warm InGaAs Sensors: Applications for Time-Domain Astronomy

We describe test observations made with a customized 640 x 512 pixel Indium Gallium Arsenide (InGaAs) prototype astronomical camera on the 100" DuPont telescope. This is the first test of InGaAs as a cost-effective alternative to HgCdTe for research-grade astronomical observations. The camera exhibits an instrument background of 113 e-/sec/pixel (dark + thermal) at an operating temperature of -40C for the sensor, maintained by a simple thermo-electric cooler. The optical train and mechanical structure float at ambient temperature with no cold stop, in contrast to most IR instruments which must be cooled to mitigate thermal backgrounds. Measurements of the night sky using a reimager with plate scale of 0.4 arc seconds / pixel show that the sky flux in Y is comparable to the dark current. At J the sky brightness exceeds dark current by a factor of four, and hence dominates the noise budget. The sensor read noise of ~43e- falls below sky+dark noise for exposures of t>7 seconds in Y and 3.5 seconds in J. We present test observations of several selected science targets, including high-significance detections of a lensed Type Ia supernova, a type IIb supernova, and a z=6.3 quasar. Deeper images are obtained for two local galaxies monitored for IR transients, and a galaxy cluster at z=0.87. Finally, we observe a partial transit of the hot JupiterHATS34b, demonstrating the photometric stability required over several hours to detect a 1.2% transit depth at high significance. A tiling of available larger-format sensors would produce an IR survey instrument with significant cost savings relative to HgCdTe-based cameras, if one is willing to forego the K band. Such a camera would be sensitive for a week or more to isotropic emission from r-process kilonova ejecta similar to that observed in GW170817, over the full 190 Mpc horizon of Advanced LIGO's design sensitivity for neutron star mergers.Comment: 13 pages, 12 figures, submitted to A

Automating biomedical data science through tree-based pipeline optimization

Over the past decade, data science and machine learning has grown from a mysterious art form to a staple tool across a variety of fields in academia, business, and government. In this paper, we introduce the concept of tree-based pipeline optimization for automating one of the most tedious parts of machine learning---pipeline design. We implement a Tree-based Pipeline Optimization Tool (TPOT) and demonstrate its effectiveness on a series of simulated and real-world genetic data sets. In particular, we show that TPOT can build machine learning pipelines that achieve competitive classification accuracy and discover novel pipeline operators---such as synthetic feature constructors---that significantly improve classification accuracy on these data sets. We also highlight the current challenges to pipeline optimization, such as the tendency to produce pipelines that overfit the data, and suggest future research paths to overcome these challenges. As such, this work represents an early step toward fully automating machine learning pipeline design.Comment: 16 pages, 5 figures, to appear in EvoBIO 2016 proceeding

Gene Therapy for Inherited Retinal and Optic Nerve Degenerations

Introduction: The eye is a target for investigational gene therapy due to the monogenic nature of many inherited retinal and optic nerve degenerations (IRD), its accessibility, tight blood-ocular barrier, the ability to non-invasively monitor for functional and anatomic outcomes, as well as its relative immune privileged state.Vectors currently used in IRD clinical trials include adeno-associated virus (AAV), small single-stranded DNA viruses, and lentivirus, RNA viruses of the retrovirus family. Both can transduce non-dividing cells, but AAV are non-integrating, while lentivirus integrate into the host cell genome, and have a larger transgene capacity. Areas covered: This review covers Leber’s congenital amaurosis, choroideremia, retinitis pigmentosa, Usher syndrome, Stargardt disease, Leber’s hereditary optic neuropathy, Achromatopsia, and X-linked retinoschisis. Expert opinion: Despite great potential, gene therapy for IRD raises many questions, including the potential for less invasive intravitreal versus subretinal delivery, efficacy, safety, and longevity of response, as well as acceptance of novel study endpoints by regulatory bodies, patients, clinicians, and payers. Also, ultimate adoption of gene therapy for IRD will require widespread genetic screening to identify and diagnose patients based on genotype instead of phenotype

CHIRON - A Fiber Fed Spectrometer for Precise Radial Velocities

The CHIRON optical high-resolution echelle spectrometer was commissioned at the 1.5m telescope at CTIO in 2011. The instrument was designed for high throughput and stability, with the goal of monitoring radial velocities of bright stars with high precision and high cadence for the discovery of low-mass exoplanets. Spectral resolution of R=79,000 is attained when using a slicer with a total (including telescope and detector) efficiency of 6% or higher, while a resolution of R=136,000 is available for bright stars. A fixed spectral range of 415 to 880 nm is covered. The echelle grating is housed in a vacuum enclosure and the instrument temperature is stabilized to +-0.2deg. Stable illumination is provided by an octagonal multimode fiber with excellent light-scrambling properties. An iodine cell is used for wavelength calibration. We describe the main optics, fiber feed, detector, exposure-meter, and other aspects of the instrument, as well as the observing procedure and data reduction.Comment: 15 pages, 10 figures. Accepted by PAS

Finite-Size-Scaling at the Jamming Transition: Corrections to Scaling and the Correlation Length Critical Exponent

We carry out a finite size scaling analysis of the jamming transition in frictionless bi-disperse soft core disks in two dimensions. We consider two different jamming protocols: (i) quench from random initial positions, and (ii) quasistatic shearing. By considering the fraction of jammed states as a function of packing fraction for systems with different numbers of particles, we determine the spatial correlation length critical exponent $\nu\approx 1$, and show that corrections to scaling are crucial for analyzing the data. We show that earlier numerical results yielding $\nu<1$ are due to the improper neglect of these corrections.Comment: 5 pages, 4 figures -- slightly revised version as accepted for Phys. Rev. E Rapid Communication