ПОЛУЧЕНИЕ РЕКОМБИНАНТНОГО ЛАКТОФЕРРИНА ЧЕЛОВЕКА ИЗ МОЛОКА КОЗ-ПРОДУЦЕНТОВ И ЕГО ФИЗИОЛОГИЧЕСКИЕ ЭФФЕКТЫ

Primary male-goats Lac-1 (human lactoferrin gene construction hLF5) and Lac-2 (human lactoferrin gene construction hLF3) with a genome containing a human lactoferrin gene were bred and the sperm bank of primary male-goats was created within the framework of Belarus-Russia Union State programs. The herd of goats that produced recombinant human lactoferrin (rhLF) in their milk was obtained. Human lactoferrin (rhLF) from the milk of transgenic goats, natural human lactoferrin (nhLf) from the woman milk, and natural goat lactoferrin (gLf) from the milk of non-transgenic goats were purified using cation-exchange chromatography. Physicochemical characteristics of rhLF were similar to those of nhLf as revealed by different analytical methods including mass spectrometry and peptide mapping. g. Oral supplementation with rhLF activated lipid metabolism and steroidogenesis, promoted favorable changes in the gut microflor composition as well as the enhancement of the functional activity and the fine structure of the rat liver and gut.В рамках белорусско-российских программ Союзного государства получены трансгенные по гену лактоферрина человека первичные козлы Лак-1 (генная конструкция hLF5) и Лак-2 (генная конструкция hLF3), создано стадо животных-продуцентов и банк спермы. Используя катионообменную хроматографию, выделены электрофоретически гомогенные препараты рекомбинантного лактоферрина человека (rhLF) из молока трансгенных коз и природного лактоферрина человека (nhLf) из женского молока. Различными аналитическими методами, включая масс-спектрометрию и пептидное картирование, показана аналогия физико-химических характеристик между rhLF и nhLf. При системном употреблении крысами  rhLF оказывал благоприятное действие на кишечную микрофлору, ультраструктуру печени и кишечника, активировал липидный метаболизм и стероидогенез

Prospects of the development of rural tourism in the conditions of silvicultural production by the way of creation lowbush blueberry (Vaccinium angustifolium Ait.) plantations

Prospects of development of rural tourism in the conditions of silvicultural production by creation plantations of lowbush blueberry are caused by: existence of a significant amount of the lands (50,000 ha of the areas of the developed riding peat bogs) corresponding for cultivation of the berry; low financial expenses on creation and maintenance of plantations; simplicity of receiving landing material; relatively rapid entry into the stage of industrial fruiting of species (for the third year after landing); high berry productivity of plantations (7.9 t of berries from a hectare for the fourth year of fructification); valuable biochemical structure of fruits

Strong-Field Ionization and Coulomb Explosion of Chlorine Weakly Bound to Small Water Clusters

Clusters exhibit an enhancement in ionization rates under intense, ultrafast laser pulses compared to their molecular/atomic counterparts Studies of ionization enhancement of weakly bound molecules to clusters have not been previously characterized and quantified. We demonstrate that weakly bound ClO to (H2O)(n) (n = 1-12) clusters and weakly bound HCl to (H2O)(n) (n = 1-12) clusters produce high atomic charge states of chlorine via Coulomb explosion. Density functional theory (DFT) was used to qualitatively compare the interaction energy of ClO with respect to the number of water molecules as well as HCl with respect to the number of water molecules. The chlorine ion signal intensity for each atomic charge state was observed to be dependent on the molecule-cluster bond strength. The observed ionization enhancement was quantified using semiclassical tunneling theory, and it was found that the Cl3+-5+ and O2+ charge states are enhanced in ionization. Possible mechanisms of ionization enhancement are explored for weakly bound chlorine species