Current Challenges in Understanding the Story of Skin Pigmentation — Bridging the Morpho-Anatomical and Functional Aspects of Mammalian Melanocytes

Melanocytes are specialized dendritic melanin producing pigment cells, which have originated from the pluripotent embryonic cells and are termed as neural crest cells (NCC). The primary locations of these cells are basal layer of epidermis and hair follicles. Besides this, they are also found in the inner ear, nervous system, and heart with spatial specific functions. There are other cells able to produce melanin but of different embryonic origin (pigmented epithelium of retina, some neurons, and adipocytes). Melanocytes of the epidermis and hair are cells which share some common structural features but in general they form biologically different populations living in unique niches of the skin. Ultra structurally, melanocytes differ from each other on the basis of their locations and function. Principal function of epidermal melanocytes is photoprotection and thermoregulation by packaging melanin pigment into melanosomes and delivering them to neighboring keratinocytes. It is unfair to think that melanocytes reap all the glory for their role in pigmenting the skin and providing it critical protection against UV damage. They probably play a significant role in diverse physiological functions and their particular functions in all target places are much wider than the melanin synthesis only. Alternation in any structure and function of these pigmentary cells affects the process of pigmentation/melanogenesis which leads to pigmentary disorders like hyperpigmentation or hypopigmentation

Polyphenols, Flavonoids, Mineral Elements, and Biological Activities of Ginger and Cinnamon Essential Oil and Extracts as Regulated by Their Isolation Procedures

Our research compared the chemical make-up of wild Ginger and cinnamon, including their essential oils (EOs), total phenol, and total flavonoid, for their antioxidant and antibacterial effects in vitro. The mineral (nutritional and poisonous) components of the plant were also identified in this investigation. Hydro distillation (HD), steam distillation (SD), and microwave-assisted distillation (MAD) were used to extract the EOs, and gas chromatography with flame ionization detection (GC-FID) and mass spectrometry detection (GC-MS) were used to examine them. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to evaluate the EOs' antioxidant properties. The essential oil was analyzed, and twenty-six components were found to make up 97.73% of the oil with a yield of 0.202%. The primary components were pulegone (74.81%), menthone (13.01%) and piperitone (3.82%). Neutron activation analysis (INAA) and inductively coupled plasma optical emission spectrometry (ICP-OES) were used to detect twenty-one elements, including macro- and micro-elements (Ba, Br, Ca, Cl, Co, Cr, Cs, Eu, Fe, K, Mg, Mn, Mo, Na, Rb, Sb, Sc, Sr, Th, U, and Zn), with the mineral element concentration being very close to the FAO recommendation

24 Hours chronomics of ambulatory blood pressure and its relation with circadian rhythm of 6-sulfatoxy melatonin in night shift health care workers

Background: Night shift workers have altered circadian pattern of blood pressure/heart rate and hormones like melatonin and cortisol. Due to this variation, night shift worker suffers from various cardiovascular disorders and hormonal disturbances.Methods: The Present study was aimed to investigate the effects of rotating night shift on 24 hours chronomics of BP/HR and its relation with 6-sulfatoxy melatonin levels. 62 healthy nursing professionals, aged 20-40 year, performing day and night shift duties were recruited. Each month scheduled to continuous 9 days night shift (12 hours in regular 9 nights, from 20:00 to 08:00); after 9 days night shift they perform remaining duties in day shift and 4 days off in each month.Results: Ambulatory BP and HR were recorded at every 30 min intervals in day time and each hour in night time synchronically with circadian pattern of 6 sulfatoxy melatonin during shift duties. Highly Significant difference was found in double amplitude (2DA) of blood pressure between night and day shift (p<0.001). In night shift, hyperbaric index (HBI) of mean systolic blood pressure was found to be increased at 00-03 am (midnight) while during day shift, peak was found at 06-09 am. Peak melatonin was to be found in early morning as compared to mid night in both the shift.Conclusions: The present study concluded that the desynchronization was appeared during night shift and entrainment of circadian rhythm in the day shift.

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Trigonella foenum graecum L. (Fenugreek) is a valuable medicinal plant cultivated for decades for its therapeutic characteristics. Still no pronounced improvement concerning wild form was accomplished as it is a self-pollinating crop. Induced mutagenesis is encouraged as a remarkable tool on this plant to circumvent the genetic bottleneck of cultivated germplasms. As a result, novel allelomorphic combinations for short-term agronomic attributes were developed. Fenugreek cultivar Pusa Early Bunching, selected for the present experiment, was mutagenized with five doses (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) of caffeine and sodium azide (SA) to evaluate its impact on the qualitative and quantitative traits of M1 and M2 generation conducted in a Complete Randomized Block Design (CRBD), replicated five times during 2019–2020 and 2020–2021, respectively. The frequency of induced phenotypic variations was assessed in M2 progenies, resulting in the identification and isolation of a broad spectrum of mutants with altered phenotypes. Mutagenic effectiveness and efficiency were found to be maximum at lower concentrations of the mutagen treatments and highest in SA, followed by caffeine. Various morphological mutants with modified characters were observed at different concentrations in M2 generation. The spectrum of mutations was wider in SA than in caffeine, as caffeine produced 51 while SA produced 54 individual mutants under seven major categories. The maximum frequency of morphological mutants was associated with leaf, followed by plant size, plant growth habit, pod, seed size, seed shape, and seed color. Morphological and structural variations in the guard cells of stomata and seeds were observed through scanning electron microscopy. The variations created in the economically important traits may enrich the genetic diversity of this plant species. Moreover, these morphological mutants may serve as a source of elite genes in further breeding programs of fenugreek

Dynamic Load Balancing for Massively Multiplayer Online Games Using OPNET

In recent years, there has been an important growth of online gaming. Today’s Massively Multiplayer Online Games (MMOGs) can contain millions of synchronous players scattered across the world and participating with each other within a single shared game. Traditional Client/Server architectures of MMOGs exhibit different problems in scalability, reliability, and latency, as well as the cost of adding new servers when demand is too high. P2P architecture provides considerable support for scalability of MMOGs. It also achieves good response times by supporting direct connections between players. In this paper, we have proposed a novel dynamic load balancing for massively multiplayer online games (MMOGs) based this hybrid Peer-to-Peer architecture. We have divided the game world space into several regions. Each region in the game world space is controlled and managed by using both a super-peer and a clone-super-peer. The region's super-peer is responsible for distributing the game update among the players inside the region, as well as managing the game communications between the players. However, the clone-super-peer is responsible for controlling the players' migration from one region to another, in addition to be the super-peer of the region when the super-peer leaves the game. We have designed and evaluated the dynamic load balancing for MMOGs based on hybrid P2P architecture. We have used OPNET Modeler 18.0 to simulate and evaluate the proposed system. Our dynamic load balancer is responsible for distributing the load among the regions in the game world space. The position of the load balancer is located between the game server and the regions. The results, following extensive experiments, show that low delay and higher traffic communication can be achieved using dynamic load balancing for MMOGs based on hybrid P2P system

Methylglyoxal Mediates Adipocyte Proliferation by Increasing Phosphorylation of Akt1

Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of the most important factors to cause metabolic syndrome. An increased MG accumulation was observed in the adipose tissue of obese Zucker rats. Cell proliferation assay showed that 5–20 µM of MG stimulated the proliferation of 3T3-L1 cells. Further study suggested that accumulated-MG stimulated the phosphorylation of Akt1 and its targets including p21 and p27. The activated Akt1 then increased the activity of CDK2 and accelerated the cell cycle progression of 3T3-L1 cells. The effects of MG were efficiently reversed by advanced glycation end product (AGE) breaker alagebrium and Akt inhibitor SH-6. In summary, our study revealed a previously unrecognized effect of MG in stimulating adipogenesis by up-regulation of Akt signaling pathway and this mechanism might offer a new approach to explain the development of obesity

Multiple Roles for the Non-Coding RNA SRA in Regulation of Adipogenesis and Insulin Sensitivity

Peroxisome proliferator-activated receptor-γ (PPARγ) is a master transcriptional regulator of adipogenesis. Hence, the identification of PPARγ coactivators should help reveal mechanisms controlling gene expression in adipose tissue development and physiology. We show that the non-coding RNA, Steroid receptor RNA Activator (SRA), associates with PPARγ and coactivates PPARγ-dependent reporter gene expression. Overexpression of SRA in ST2 mesenchymal precursor cells promotes their differentiation into adipocytes. Conversely, knockdown of endogenous SRA inhibits 3T3-L1 preadipocyte differentiation. Microarray analysis reveals hundreds of SRA-responsive genes in adipocytes, including genes involved in the cell cycle, and insulin and TNFα signaling pathways. Some functions of SRA may involve mechanisms other than coactivation of PPARγ. SRA in adipocytes increases both glucose uptake and phosphorylation of Akt and FOXO1 in response to insulin. SRA promotes S-phase entry during mitotic clonal expansion, decreases expression of the cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1, and increases phosphorylation of Cdk1/Cdc2. SRA also inhibits the expression of adipocyte-related inflammatory genes and TNFα-induced phosphorylation of c-Jun NH2-terminal kinase. In conclusion, SRA enhances adipogenesis and adipocyte function through multiple pathways

ADDRESSING THE GAPS IN ASSESSMENT AND TREATMENT OF THE UPPER EXTREMITY IN REHABILITATION OF INDIVIDUALS WITH NEUROLOGICAL CONDITIONS

Regaining upper extremity function is critical following stroke and spinal cord injury (SCI). The objectives of this thesis were to develop and assess the psychometric properties of a 3D printed version of an upper extremity outcome assessment tool called the Toronto Rehabilitation Institute-Hand Function Test (TRI-HFT) and to assess the feasibility of stimulating interscapular muscles using transcutaneous functional electrical stimulation (FES). The first study explored the feasibility of 3D printing the original TRI-HFT objects and assessed its inter and intra-rater reliability and convergent validity in chronic stroke. The second study focused on assessing its psychometric properties in the sub-acute and chronic SCI populations. We hypothesized that the TRI-HFT could be 3D printed and that the 3D printed test would have high reliability and validity in stroke and SCI populations. In the third study I explored the feasibility and benefits of stimulating the Lower Trapezius (LT), Serratus Anterior (SA) and Upper Trapezius (UT) along with Anterior Deltoid during forward flexion and along with Middle Deltoid during abduction in able-bodied individuals. The underlying hypothesis was that it would result in an increased range and more natural reaching movement. In the first and second study we found that all objects of the TRI-HFT could be successfully 3D printed with an error margin of less than 10% except for the Paper and the Sponge objects. The 3D TRI-HFT showed high inter and intra-rater reliability in stroke and SCI.The 3D TRI-HFT showed strong criterion validity when compared to the Graded Redefined Assessment of Strength, Sensibility and Prehension test in the SCI population. The 3D TRI-HFT showed moderate to strong construct validity when compared to the Chedoke-McMaster Stroke Assessment-Arm and Hand and the Fugl Meyer Assessment-Hand in chronic stroke. In the third study, the LT, SA and UT could be successfully stimulated using surface FES. The maximum reach in abduction for FES of middle deltoid along with the interscapular muscles was 51.77°±17.54° compared to FES for middle deltoid alone which was 43.76°±15.32°. This work essentially builds on the current state of assessment and FES treatment of the upper extremity in the rehabilitation domain.Ph.D