Detection of HPV in cancerous and non-cancerous esophageal tissues from Turkmen-Sahra, Iran

The aim of this study were to assess the presence of HPV esophageal infection among Iranian Turkmen who live in an area located in the cancer belt in Asia. The specimens derived from 120 patients previously diagnosed for Squamous Cell Carcinoma (SCC) non-cancerous tissue derived from esophagus. All specimens were examined for the presence of HPV DNA PCR was utilized to amplify a 150 bp segment of HPV L1 gene using the consensus primers. The amplified region was subsequently sequenced to identify the HPV genotypes. The HPV DNA was detected in 49.4% of patients with SCC, and 58% of non-cancerous tissue of esophagus. The positive samples included HPV-16 (46.6%), HPV-6 (24.6%), HPV-66 (8.2%), HPV-52 (4.1%), HPV-18 (2.7%); 14% of cases were positive for more than one type of HPV. The results confirm the presence of HPV in both esophageal cancerous and non-cancerous tissues. These results imply two different interpretations: 1) Due to non-significant difference between the rate of HPV positive in cancerous and non-cancerous tissues, HPV has no important role in esophageal cancers, which is less probable. 2). Because of a highly incidence of esophageal cancer in Turkmen-Sahra region, the HPV is a possible etiologic agent in esophageal carcinogenesis, most probably acting synergistically with physical, chemical, and/or nutritional factors that have previously been found to be related to this malignancy in Turkmen-Sahra. © 2006 Academic Journals Inc., USA

Investigation of antibiotic resistance and frequency of Clostridium difficile tcdA and tcdB genes in feces of calves in Chaharmahal and Bakhtiari province

زمینه و هدف: کلستریدیوم دیفیسیل یک باسیل گرم مثبت و اسپورزا می باشد که قادر به ایجاد بیماری در انسان و حیوانات است. هدف از مطالعه حاضر بررسی فراوانی ژن های ویرولانس و مقاومت آنتی بیوتیکی کلستریدیوم دیفیسیل جدا شده از مدفوع گوساله ها در استان چهارمحال و بختیاری می باشد. روش بررسی: در این مطالعه مقطعی- توصیفی تعداد 150 نمونه مدفوع تازه گوساله جمع آوری و کلستریدیوم دیفیسیل به کمک روش های کشت باکتریایی جداسازی شد. DNA ژنومی باکتری ها با استفاده از کیت استخراج DNA، تخلیص و ژن های tcdA و tcdB با استفاده از روش Multiplex PCR شناسایی گردید. برای بررسی مقاومت دارویی از روش انتشار دیسک به روش Kirby-Bauer استفاده گردید. یافته ها: تعداد 90 نمونه (60) دارای کلستریدیوم دیفیسیل بوده که از این تعداد، 8 نمونه (8/8 ) دارای ژن tcdA و 16 نمونه (7/17) ژن tcdB داشتند. در آزمایش مقاومت آنتی بیوتیکی مشاهده شد که بیشترین میزان مقاومت، به ترتیب مربوط به آنتی بیوتیک های کلیندامایسن (100) و اریترومایسین (90) است و بیشترین حساسیت به ترتیب مربوط به سیپروفلوکساسین (50) و وانکومایسین (20) می باشد. نتیجه گیری: با توجه به نتایج مطالعه حاضر کلستریدیوم دیفیسیل دارای شیوع و مقاومت آنتی بیوتیکی بالا بوده که می بایست با اتخاذ روش های پیشگیری و درمانی مناسب و همچنین محدود کردن استفاده از داروهای ضد میکروبی در انسان و دام از توسعه ی این باکتری و به خصوص سویه های بیماریزا جلوگیری کرد

Weyl holographic superconductor in the Lifshitz black hole background

We investigate analytically the properties of the Weyl holographic superconductor in the Lifshitz black hole background. We find that the critical temperature of the Weyl superconductor decreases with increasing Lifshitz dynamical exponent, $z$, indicating that condensation becomes difficult. In addition, it is found that the critical temperature and condensation operator could be affected by applying the Weyl coupling, $\gamma$. Moreover, we compute the critical magnetic field and investigate its dependence on the parameters $\gamma$ and $z$. Finally, we show numerically that the Weyl coupling parameter $\gamma$ and the Lifshitz dynamical exponent $z$ together control the size and strength of the conductivity peak and the ratio of gap frequency over critical temperature $\omega_{g}/T_{c}$.Comment: 25 pages, 22 figure

Detection of human papillomavirus DNA in intraosseus ameloblastoma

Human Papillomavirus (HPV) infection has been shown as a risk factor in oral carcinogenesis. The association between HPV and benign and malignant neoplasm of oral mucosa, especially surface epithelium-derived tumors, is well established. The role of HPV in pathogenesis of odontogenic cysts and tumors has been published in few articles. The aim of this study was detection of HPV in Iranian patients with intrabony ameloblastoma and investigation of specific risk factors associated with ameloblastoma. One hundred intrabony ameloblastoma and 50 age-sex matched samples as controls were evaluated by polymerase chain reaction for the detection and typing of HPV. Fisher exact and chi square tests were used to assess the data. HPV DNA was detected in 32% of patients and 10% of controls. HPV-6 was the most prevalent genotype (31.6%) in infected cases. It was followed by HPV-11 (12.5%), HPV-16 (12.5%) and HPV-31 (3.1%). We found a significant association between presence of HPV and location of tumor (p = 0.02), traumatic history (p = 0.03) and ododontic therapy (p = 0.01). These findings indicated that HPV-6 probably is one of the most important etiologic agents in causing intraosseous ameloblastoma in Iranian population. © 2006 Academic Journals Inc., USA