Pilot phase trial to quantify the extent and relevance of any deepwater puerulus settlement that may have taken place in the Western Rocklobster Fishery

This study had two objectives. Firstly to trial modifications to commercial western rock lobster pots, so as to sample as wide a size range of lobsters on the grounds as possible. Sampling took place over a 10 day period between 13-24 September 2009, with seven commercial fishermen being responsible for collection of the data at different sampling locations (Mandurah, Fremantle, Lancelin, Dongara, Geraldton, Abrolhos and Kalbarri). This arrangement proved to be successful in that a wide area of the coast was covered by the sampling regime and good quality catch composition data was achieved across the four depth zones (0-10 fm, 10-20 fm, 20-30 fm and >30 fm) that were sampled. The different pot modifications that were trialled sampled a wide size range (25-144 mm CL), but mostly caught lobsters around the legal size limit (modal size, 80 mm CL). In three of the four areas where more than one pot type was used, there were significant differences (p<0.01) in the catch rates of one or more size classes between the various pot types.The second objective was to establish whether there has been a shift in post-puerulus settlement from shallow to deeper waters. Analysis showed that depth was highly significant (p<0.05) in determining the catch rates of most size classes (≤50 mm CL; 51-65 mm CL; 66-76 mm CL; 77-105 mm CL and ≥105 mm CL). Generally, small size classes were sampled in the shallow depth categories and larger lobsters were sampled in the deeper depth categories, which would suggest that as in the past, recent puerulus settlement has been in shallow depths. Accordingly, it would seem reasonable to conclude that it is unlikely that there has been a major shift in the depths at which pueruli are settling. However, small lobsters were sampled in low numbers in the deep water categories in this survey and this therefore does not exclude the possibility that deep water settlement may be becoming more common than in past.In terms of recommendations, more monitoring of post-puerulus/juvenile lobsters using modified commercial pots similar to those used in this study has the potential to provide inter-annual comparisons of the numbers of small size classes settling at different depths on the grounds. It is only with more data of this type over a longer time period, that it will be possible to show whether there is any indirect evidence of a shift in settlement to deeper water. Should this work continue into the future, it would be beneficial to standardize on a consistent type of modification to the commercial pots so that more reliable comparisons of the relative numbers of juvenile animals can be made between areas

EXTRACTION OF VINEYARD MACROSTRUCTURE FROM SUB-OPTIMAL SEQUENCES OF AERIAL IMAGERY

Remote sensing techniques can be used to identify and classify vine properties such as row width, height, cover-fraction, missing segments and leaf area density, providing opportunities to visualise plant vigour as a spatial function of vineyard geography. This information may then be integrated into decision support tools to improve vineyard management practices. An algorithm for identifying vines from a sequence of overlapping aerial images and then estimating their properties is described. The image stacks were obtained from visible and long wave infrared cameras carried by an unmanned aerial vehicle (UAV). Structure from motion (SfM) was used to create 3D thermal and colourised point clouds, from which the underlying topography of the surface terrain was extracted. The surface topographic model was obtained using bounded data query nearest neighbour calculations, which were reduced to computationally manageable levels using Kd-trees that recursively partitioned the point clouds by progressively separating them into binary trees. This allowed the point clouds to be classified in terms of their hue, saturation, surface temperature and height relative to surface topography using Lloyd’s unsupervised k-means clustering. Individual samples were then associated using Gaussian probability density functions normalised by cluster statistics. The algorithm was evaluated against ground truth obtained using aerial data in terms of its accuracy and robustness using a combination of real world conditions that included high shadowing, poor contrast and UAV flight paths and camera settings that delivered sub-optimal SfM performance

Australian encephalitis: sentinel chicken surveillance programme

Sentinel chicken flocks are used to monitor flavivirus activity in Australia. The main viruses of concern are Murray Valley encephalitis (MVE) and Kunjin which cause the potentially fatal disease encephalitis, in humans. Currently 30 flocks are maintained in the north of Western Australia, 9 in the Northern Territory, 12 in New South Wales and 10 in Victoria. The flocks in Western Australia and the Northern Territory are tested year round but those in New South Wales and Victoria are tested only from November to March, during the main risk season. Results are coordinated by the Arbovirus Laboratory in Perth and reported bimonthly

Sentinel chicken surveillance programme In Australia, 1 July 2001 to 30 June 2002

Detection of flavivirus seroconversions in sentinel chicken flocks located throughout Australia is used to provide an early warning of increased levels of Murray Valley encephalitis (MVE) and Kunjin (KUN) virus activity in the region. During the 2002-2003 season low levels of flavivirus activity were detected in northern Australia compared to previous years. MVE and KUN virus activity was detected in the Kimberley and Pilbara regions of Western Australia and the Northern Territory but not in north Queensland, New South Wales or Victoria. This is similar to the previous season. There were no reported cases of diseas disease caused by either virus reported

Identifying hybridization and admixture using SNPs:Application of the DArTseq platforminphylogeographic research on vertebrates

Next-generation sequencing (NGS) approaches are increasingly being used to generate multi-locus data for phylogeographic and evolutionary genetics research. We detail the applicability of a restriction enzyme-mediated genome complexity reduction approach with subsequent NGS (DArTseq) in vertebrate study systems at different evolutionary and geographical scales. We present two case studies using SNP data from the DArTseq molecular marker platform. First, we used DArTseq in a large phylogeographic study of the agamid lizard Ctenophorus caudicinctus, including 91 individuals and spanning the geographical range of this species across arid Australia. A low-density DArTseq assay resulted in 28 960 SNPs, with low density referring to a comparably reduced set of identified and sequenced markers as a cost-effective approach. Second, we applied this approach to an evolutionary genetics study of a classic frog hybrid zone (Litoria ewingii–Litoria paraewingi) across 93 individuals, which resulted in 48 117 and 67 060 SNPs for a low- and high-density assay, respectively. We provide a docker-based workflow to facilitate data preparation and analysis, then analyse SNP data using multiple methods including Bayesian model-based clustering and conditional likelihood approaches. Based on comparison of results from the DArTseq platform and traditional molecular approaches, we conclude that DArTseq can be used successfully in vertebrates and will be of particular interest to researchers working at the interface between population genetics and phylogenetics, exploring species boundaries, gene exchange and hybridization. © 2017 The Authors

Some comments on graduate training for engineers

QUESTIONING the value and adequacy of postgraduate engineering courses as commonly conducted, L. W. W. Morrow in an article in Electrical Engineering for March 1939, pages 118–22, called for a program of investigation of postgraduate training. Solicited comments of some representative industrialists and educators are presented here

Manipulating the Mouse Genome to Engineer Precise Functional Syntenic Replacements with Human Sequence

SummaryWe have devised a strategy (called recombinase-mediated genomic replacement, RMGR) to allow the replacement of large segments (>100 kb) of the mouse genome with the equivalent human syntenic region. The technique involves modifying a mouse ES cell chromosome and a human BAC by inserting heterotypic lox sites to flank the proposed exchange interval and then using Cre recombinase to achieve segmental exchange. We have demonstrated the feasibility of this approach by replacing the mouse α globin regulatory domain with the human syntenic region and generating homozygous mice that produce only human α globin chains. Furthermore, modified ES cells can be used iteratively for functional studies, and here, as an example, we have used RMGR to produce an accurate mouse model of human α thalassemia. RMGR has general applicability and will overcome limitations inherent in current transgenic technology when studying the expression of human genes and modeling human genetic diseases

A qualitative study of stakeholders' perspectives on the social network service environment

Over two billion people are using the Internet at present, assisted by the mediating activities of software agents which deal with the diversity and complexity of information. There are, however, ethical issues due to the monitoring-and-surveillance, data mining and autonomous nature of software agents. Considering the context, this study aims to comprehend stakeholders' perspectives on the social network service environment in order to identify the main considerations for the design of software agents in social network services in the near future. Twenty-one stakeholders, belonging to three key stakeholder groups, were recruited using a purposive sampling strategy for unstandardised semi-structured e-mail interviews. The interview data were analysed using a qualitative content analysis method. It was possible to identify three main considerations for the design of software agents in social network services, which were classified into the following categories: comprehensive understanding of users' perception of privacy, user type recognition algorithms for software agent development and existing software agents enhancement