StoRM: A Manager for Storage Resource in Grid

Nowadays, data intensive applications demand high-performance and large-storage systems capable of serving up to various Petabytes of storage space. Therefore, common solutions adopted in data centres include Storage Area Networks (SAN) and cluster parallel file systems, such as GPFS from IBM and Lustre from Sun Microsystems. In order to make these storage system solutions available in modern Data Grid architectures, standard interfaces are needed. The Grid Storage Resource Manager (SRM) interface is one of these standard interfaces. Grid storage services implementing the SRM standard provide common capabilities and advanced functionality such as dynamic space allocation and file management on shared storage systems. In this paper, we describe StoRM (STOrage Resource Manager). StoRM is a flexible and high-performing implementation of the standard SRM interface version 2.2. The software architecture of StoRM allows for an easy integration to different underlying storage systems via a plug-in mechanism. In particular, StoRM takes advantage from storage systems based on cluster file systems. Currently, StoRM is installed and used in production in various data centres, including the WLCG Italian Tier-1. In addition, Economics and Financial communities, as represented by the EGRID Project, adopt StoRM in production as well

An efficient strategy for the collection and storage of large volumes of data for computation

In recent years, there has been an increasing amount of data being produced and stored, which is known as Big Data. The social networks, internet of things, scientific experiments and commercial services play a significant role in generating a vast amount of data. Three main factors are important in Big Data; Volume, Velocity and Variety. One needs to consider all three factors when designing a platform to support Big Data. The Large Hadron Collider (LHC) particle accelerator at CERN consists of a number of data-intensive experiments, which are estimated to produce a volume of about 30 PB of data, annually. The velocity of these data that are propagated will be extremely fast. Traditional methods of collecting, storing and analysing data have become insufficient in managing the rapidly growing volume of data. Therefore, it is essential to have an efficient strategy to capture these data as they are produced. In this paper, a number of models are explored to understand what should be the best approach for collecting and storing Big Data for analytics. An evaluation of the performance of full execution cycles of these approaches on the monitoring of the Worldwide LHC Computing Grid (WLCG) infrastructure for collecting, storing and analysing data is presented. Moreover, the models discussed are applied to a community driven software solution, Apache Flume, to show how they can be integrated, seamlessly

Scheme for sub-shot-noise transmission measurement using a time multiplexed single-photon source

A promising result from optical quantum metrology is the ability to achieve sub-shot-noise performance in transmission or absorption measurements. This is due to the significantly lower uncertainty in light intensity of quantum beams with respect to their classical counterparts. In this work, we simulate the outcome of an experiment that uses a multiplexed single-photon source based on pair generation by continuous spontaneous parametric down conversion (SPDC) followed by a time multiplexing set-up with a binary temporal division strategy, considering several types of experimental losses. With such source, the sub-Poissonian statistics of the output signal is the key for achieving sub-shot-noise performance. We compare the numerical results with two paradigmatic limits: the shot-noise limit (achieved using coherent sources) and the quantum limit (obtained with an ideal photon-number Fock state as the input source). We also investigate conditions in which threshold detectors can be used, and the effect of input light fluctuations on the measurement error. Results show that sub-shot-noise performance can be achieved, even without using number-resolving detectors, with improvement factors that range from 1.5 to 2. This technique would allow measurements of optical absorption of a sample with reasonable uncertainty using ultra-low light intensity and minimum disruption of biological or other fragile specimens.Comment: 10 pages, 8 figure

Biomimetic Electrospun Self-Assembling Peptide Scaffolds for Neural Stem Cell Transplantation in Neural Tissue Engineering

Spinal cord regeneration using stem cell transplantation is a promising strategy for regenerative therapy. Stem cells transplanted onto scaffolds that can mimic natural extracellular matrix (ECM) have the potential to significantly improve outcomes. In this study, we strived to develop a cell carrier by culturing neural stem cells (NSCs) onto electrospun 2D and 3D constructs made up of specific crosslinked functionalized self-assembling peptides (SAPs) featuring enhanced biomimetic and biomechanical properties. Morphology, architecture, and secondary structures of electrospun scaffolds in the solid-state and electrospinning solution were studied step by step. Morphological studies showed the benefit of mixed peptides and surfactants as additives to form thinner, uniform, and defect-free fibers. It has been observed that β-sheet conformation as evidence of self-assembling has been predominant throughout the process except for the electrospinning solution. In vitro NSCs seeded on electrospun SAP scaffolds in 2D and 3D conditions displayed desirable proliferation, viability, and differentiation in comparison to the gold standard. In vivo biocompatibility assay confirmed the permissibility of implanted fibrous channels by foreign body reaction. The results of this study demonstrated that fibrous 2D/3D electrospun SAP scaffolds, when shaped as micro-channels, can be suitable to support NSC transplantation for regeneration following spinal cord injury

Mesenchymal stromal cells promote the proliferation of basal stem cells and efficient epithelization in organotypic models of wound healing

Adipose derived mesenchymal stromal cells (ADSCs) represent a fascinating tool in the scenario of wound healing and regenerative medicine. Recent data already demonstrated that ADSCs could exert a stimulatory action on epithelial cells through secretion of soluble factors. The aim of the present study was to assess how ADSCs guide wound re-epithelization in vitro in the presence of keratinocytes. We used an organotypic model of wound healing and we seeded keratinocytes on a ADSC-induced dermal matrix. Conventional hematoxylin–eosin stain and immunohistochemistry staining for Ki67, p63 and pan-keratins were performed at different timepoints. Histological sections of organotypic cultures showed complete coverage of the ADSC-induced matrix by keratinocytes. Proliferation of basal stem cells was found to be the main mechanism responsible for epithelization of the dermis. In conclusion, ADSC do not only stimulate dermal regeneration through collagen deposition but also promote epithelization

Mesenchymal stem cells for the treatment of psoriasis: a comprehensive review

Mesenchymal stem cells (MSCs) have recently been shown to have not only regenerative capabilities but also immunomodulating properties. For this reason, they are currently under investigation in clinical trials for the treatment of several autoimmune systemic disorders. Psoriasis is a systemic immune-mediated disease for which MSCs could have therapeutic potential. We analysed the existing literature with regard to MSC-based strategies for the treatment of psoriasis, using the MEDLINE, Embase, Scopus and Cochrane Library electronic databases from inception to the date of study. A number of studies confirm the involvement of MSCs in psoriasis pathogenesis and therefore designate MSCs as an important potential therapeutic tool in this setting. Preclinical data are mostly based on imiquimod-induced murine models of psoriasis, and confirm the anti-inflammatory and immunomodulatory action of MSCs in the setting of psoriasis. Six patients affected by psoriasis were described in four clinical studies. Despite significant differences in terms of therapeutic protocols and clinical outcomes, the MSC-based regimens were efficacious in 100% of the cases. Despite more data still being needed, MSCs could be a promising therapy for psoriasis

Which are the main fluorophores in skin and oral mucosa? A review with emphasis on clinical applications of tissue autofluorescence

Abstract OBJECTIVES: The present review provides information about which molecules appear to be the main fluorophores in skin and oral mucosa, together with their clinical applications. DESIGN: The MEDLINE database was searched, using "oral mucosa AND fluorophores", "skin AND fluorophores", "epidermal AND fluorophores", "dermal AND fluorophores" and "cutaneous AND fluorophores" as entry terms. We searched the literature following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The level of evidence in the studies was assessed using the Classification of the Oxford Centre for Evidence-based Medicine (CEBM) Levels for Diagnosis. RESULTS: Five papers and 17 were primarily focused on description of fluorophores in oral mucosa and skin Evidence exists that fluorophores of oral mucosa and skin are mainly proteins such as collagen, elastin, keratin and tryptophan. Other possible fluorophores identified are: porphyrins, advanced glycation end products, flavins, lipopigment, nicotinamide adenine dinucleotide, flavin adenine dinucleotide, pheomelanin, eumelanin and components of lipofuscin. Clinical applications of oral mucosal autofluorescence (AF) are related to management of malignant and potentially malignant lesions. In the skin, AF has been used for acne assessment, diagnosis of sweat-gland pathologies, glycemic control and management of malignant lesions and as a marker for skin aging. CONCLUSION: Fluorophores stimulated through AF devices are implied in different physiologic and pathologic processes. AF seems to be useful for several clinical applications, especially in skin department. Because most of the studies show a low level of evidence, further studies are necessary in such a promising and fascinating field