119 research outputs found
An early Emsian (Zlichovian) ammonoid assemblage from Sangibaland Mountain (Shakhimardan River Basin) (South Tien Shan, Kyrgyzstan)
Β© 2017, The Author(s). Early Emsian (Zlichovian) ammonoids are recorded from a section of neritic deposits on the western and northwestern slopes of Sangibaland Mountain (right bank of the Shakhimardan River, near the village of Jiydelik, South Fergana, South Tien Shan, Kyrgyzstan) including other faunas such as conodonts, dacryoconarids, brachiopods, trilobites, and corals. The ammonoid fauna includes Erbenoceras cf. solitarium (Barrande, 1865), E. kimi Bogoslovsky, 1980 and new species of teicherticeratids. This combination of taxa allows the host beds to be correlated with the Zlichovian LDIII Zone. Ammonoids come from the Katran Formation, from beds previously recognised as the Sandal Formation, which is wellβknown for its neritic assemblages, interpreted as inhabitants of shallow shelf zones in the northern regions of the Paleozoic AlaiβTarim Terrane. Near the end of the Zlichovian, when the Dalejan transgression spread to the Sangibaland Region, ammonoids colonised the neritic basin near a carbonate platform, already inhabited by abundant benthic fauna. The co-occurrence of neritic and pelagic organisms is a useful feature providing a basis for the correlation between the pelagic and neritic successions of the lower Emsian (Zlichovian)
Palliative Surgery for Biliary Drainage in an Unresectable Pancreatic Cancer
Pancreatic cancer, known for its rapid progression and poor prognosis, usually presents with obstructive jaundice. Biliary drainage can be achieved by various techniques and approaches, with endoscopic drainage as the preferred method. However, open drainage of the biliary tree is indicated when unresectable tumor is found during resection surgery. This is a case of biliary drainage with a double bypass biliodigestive construction, which could be performed in patients with unresectable cancer in the head of the pancreas presenting with obstructive jaundice and gastric outlet obstruction
Modification of the Method for the Determination of Zinc Content in Insulins by Atomic Absorption Spectrometry
Scientific relevance. Zinc quantification in insulin active substances and preparations by atomic absorption spectrometry requires a sample preparation procedure using hydrochloric acid, a narcotic and psychotropic precursor. The exclusion of precursors from laboratory practice significantly reduces labour costs during analysis.Aim. This study aimed to modify the pharmacopoeial method for quantifying zinc in insulins with a view to eliminating drug precursors and to evaluate the applicability of the modified method across different insulin preparations.Materials and methods. The study considered insulin active substances and preparations of various types, dosage forms, and strengths. Zinc content determination involved using an Agilent 240FS atomic absorption spectrometer at a characteristic wavelength of 213.86 nm.Results. The authors selected the conditions for elemental analysis (nitric acid concentration, monochromator slit width, and flame stabilisation time) corresponding to the pharmacopoeial requirements for quantifying zinc in insulins (RSDΒ β€1.4%, standard zinc solution with a concentration of 0.8 mg/dm3, and calibration curve correlation coefficientΒ β₯0.99). The study compared zinc content measurements in the studied insulin samples with hydrochloric acid or nitric acid as a solvent; it did not reveal any significant differences between the results obtained by the pharmacopoeial method and the modified method.Conclusions. The modified method enables analysts to quantify zinc in insulin preparations in various dosage forms without drug precursors
Optimal Location of Two Laser-interferometric Detectors for Gravitational Wave Backgrounds at 100 MHz
Recently, observational searches for gravitational wave background (GWB) have
been developed and given constraints on the energy density of GWB in a broad
range of frequencies. These constraints have already resulted in the rejection
of some theoretical models of relatively large GWB spectra. However, at 100
MHz, there is no strict upper limit from direct observation, though an indirect
limit exists due to He4 abundance due to big-bang nucleosynthesis. In our
previous paper, we investigated the detector designs that can effectively
respond to GW at high frequencies, where the wavelength of GW is comparable to
the size of a detector, and found that the configuration, a so-called
synchronous-recycling interferometer is best at these sensitivity. In this
paper, we investigated the optimal location of two synchronous-recycling
interferometers and derived their cross-correlation sensitivity to GWB. We
found that the sensitivity is nearly optimized and hardly changed if two
coaligned detectors are located within a range 0.2 m, and that the sensitivity
achievable in an experiment is far below compared with the constraint
previously obtained in experiments.Comment: 17 pages, 6 figure
Selective Quantification of Organic and Inorganic Arsenic in Kelp Thalli and Kelp-Based Products
Kelp can accumulate large quantities of arsenic compounds even in the absence of considerable environmental pollution. A substantial difference in toxicity between organic and inorganic arsenic compounds makes the form of arsenic relevant for the risk assessment of consuming kelp thalli and kelp-based products.The aim of the study was to develop an analytical procedure for the selective quantification of organic and inorganic arsenic in kelp thalli by inductively coupled plasma mass spectrometry and solid-phase extraction without scheduled precursors.Materials and methods. The authors studied samples of Laminaria saccharina and Laminaria japonica, spiking mixtures of chemical compounds containing arsenic in different oxidation states, and bioactive dietary supplements based on kelp thalli. Solid-phase extraction was performed using Maxi-Clean SAX cartridges. The arsenic content was determined using an Agilent 7900 inductively coupled plasma mass spectrometer.Results. Microwave-assisted extraction with deionised water ensures 91% recovery of arsenic-containing compounds from kelp thalli, and the addition of hydrogen peroxide to the extractant provides complete extraction. Solid-phase extraction with an eluent based on 3% H2O2 can extract the organic fraction from a mixture of organic and inorganic arsenic compounds without washing the inorganic fraction off the cartridge.Conclusions. The authors offer an effective analytical procedure for the selective quantification of organic and inorganic arsenic in kelp thalli and kelp-based products. This procedure allows for the isolation of arsenic-containing compounds from the organic matrix of kelp with 3% hydrogen peroxide. Solid-phase extraction with this extractant can effectively separate organic and inorganic fractions without prior neutralisation of the test solution
ASSOCIATION OF THE METABOLIC SYNDROME CONSTITUENTS WITH MARKERS OF SUBCLINICAL TARGET ORGAN DAMAGE DURING FOLLOW-UP OF INTELLECTUAL LABORERS
Aim. To evaluate the relation of metabolic syndrome (MS) and its constituents with markers of subclinical damage of target organs (TOD) in follow-up of almost healthy intellectual laborers.Material and methods. From the selection of 1600 employees of a bank we randomly selected 383 with at least one component of MS without cardiovascular disorders, of those by the end of 2 years period 331 came to final visit (response 86%). Mean age 46,6Β±9,0 y., mostly women (214 (64,6%)). All patients underwent anthropometry, blood pressure measurement (BP), lipids investigation, creatinine and fasting glucose, echocardiography with the assessment of the left ventricle hypertrophy (LVH), ultrasound study of carotid arteries (intima-media complex thickness β CIM, and atherosclerotic plaques), vascular rigidity assessment, anklebrachial index, albumin concentration in single portion of urine at both stages of observation.Results. While performing multiple logistic regression, presence of arterial hypertension (AH) associated with increased probability of LVH, thickening of CIM and higher vessel rigidity in standardization by gender and age. Relation of MS with the markers of TOD has not been found. In 2 years of follow-up there was a significant increase of patients with thickening of CIM (from 81 (24,5%) to 146 (44,1%), p<0,001) and decrease of LVH prevalence (from 154 (46,7%) to 109 (32,9%), p=0,003) together with significant decrease of BP and total cholesterol.Conclusion. Presence of AH is associated with higher probability of LVH and increased vessel rigidity, as atherosclerotic changes in carotid arteries. MS was not related with an increased prevalence of TOD, and the main predetermining factors for structural heart abnormalities, the vessels and kidneys, were gender and age. In 2 years of observation there was markedly decreased number of patients with LVH and kidney dysfunction at the background of BP pattern improvement, and increase of the number of patients with thicker CIM, regardless of a decrease of the hypercholesterolemia patients. In MS patients there was more common to use antihypertension treatments, that led to more prominent LVH regression
ΠΡΠΎΠ±Π»Π΅ΠΌΡ Π½ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΌΡΡΡΡΠΊΠ° Π² Π±ΡΡΡΡ Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΡΡ ΠΈ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°Ρ Π½Π° ΠΈΡ ΠΎΡΠ½ΠΎΠ²Π΅
Algae tend to accumulate elemental toxic substances in high concentrations. Algae are widely used in the food and pharmaceutical industries, and this dictates the need to establish limits for the content of toxic substances that they may contain. The aim of the study was to analyse the requirements of the Russian and foreign pharmacopoeias and other regulatory documents concerning the limits for the content of arsenic in brown algae. The paper presents the results of analysis of monographs from the State Pharmacopoeia of the Russian Federation, XIII and XIV editions, draft version of the Pharmacopoeia of the Eurasian Economic Union, United States Pharmacopoeia, Japanese Pharmacopoeia, European Pharmacopoeia, and Ayurvedic Pharmacopoeia of India containing limits for the content of arsenic in herbal medicinal products (HMPs). In addition, the authors analysed Russian, international and foreign food industry and dietary supplements regulations, as well as scientific publications on arsenic content in brown algae. They also considered the nomenclature of arsenic compounds to be determined and controlled in medicinal products, highlighted the main approaches to and identified global trends in establishing the limits for their content in HMPs. The paper summarises specific aspects of inorganic arsenic compounds accumulation by brown algae. It was demonstrated that the majority of foreign pharmacopoeias either have specific norms for arsenic content in brown algae, which differ from the norms for HMPs, or have general norms that take into account different toxicity levels of organic and inorganic arsenic compounds. There is a tendency to control the content of elemental toxic substances based on their maximum allowable daily intake. The paper substantiates the need for separate determination of toxic inorganic arsenic compounds and potentially toxic methyl arsonate and dimethyl arsinate in HMPs.ΠΠΎΡΡΠΊΠΈΠ΅ Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΠΈ ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠ²ΠΎΠΉΡΡΠ²ΠΎΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π² ΡΠ²ΠΎΠΈΡ
ΡΠΊΠ°Π½ΡΡ
ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΡΠ΅ ΡΠΎΠΊΡΠΈΠΊΠ°Π½ΡΡ Π² Π²ΡΡΠΎΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡΡ
. ΠΠΎΠ΄ΠΎΡΠΎΡΠ»ΠΈ ΡΠ²Π»ΡΡΡΡΡ ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠΌ ΡΡΡΡΠ΅ΠΌ Π² ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΈ ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ, ΠΏΠΎΡΡΠΎΠΌΡ Π²ΠΎΠ·Π½ΠΈΠΊΠ°Π΅Ρ Π²ΠΎΠΏΡΠΎΡ ΠΎ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ Π½ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² Π½ΠΈΡ
ΡΠΎΠΊΡΠΈΠΊΠ°Π½ΡΠΎΠ². Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ β Π°Π½Π°Π»ΠΈΠ· ΡΡΠ΅Π±ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ ΠΈ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΡ
ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉ ΠΈ Π΄ΡΡΠ³ΠΈΡ
Π½ΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΡ
Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠΎΠ² ΠΊ Π½ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΌΡΡΡΡΠΊΠ° Π² Π±ΡΡΡΡ
Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΡΡ
. Π ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°ΡΠ΅ΠΉ ΠΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π€Π΅Π΄Π΅ΡΠ°ΡΠΈΠΈ XIII ΠΈ XIV ΠΈΠ·Π΄Π°Π½ΠΈΠΉ, ΠΏΡΠΎΠ΅ΠΊΡΠ° ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ ΠΠ²ΡΠ°Π·ΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΎΡΠ·Π°, Π€Π°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ Π‘Π¨Π, Π―ΠΏΠΎΠ½ΡΠΊΠΎΠΉ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ, ΠΠ²ΡΠΎΠΏΠ΅ΠΉΡΠΊΠΎΠΉ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ, ΠΡΡΠ²Π΅Π΄ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΈ ΠΠ½Π΄ΠΈΠΈ, ΠΊΠ°ΡΠ°ΡΡΠΈΡ
ΡΡ Π½ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΌΡΡΡΡΠΊΠ° Π² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°Ρ
(ΠΠ Π). ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Ρ ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½Π°Ρ, ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½Π°Ρ ΠΈ Π·Π°ΡΡΠ±Π΅ΠΆΠ½Π°Ρ Π½ΠΎΡΠΌΠ°ΡΠΈΠ²Π½Π°Ρ Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠ°ΡΠΈΡ ΠΏΠΈΡΠ΅Π²ΠΎΠΉ ΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π½ΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΠ΅ Π°ΠΊΡΡ, ΡΠ΅Π³ΡΠ»ΠΈΡΡΡΡΠΈΠ΅ ΠΎΠ±ΠΎΡΠΎΡ Π±ΠΈΠΎΠ΄ΠΎΠ±Π°Π²ΠΎΠΊ, ΠΈ Π½Π°ΡΡΠ½ΡΠ΅ ΠΏΡΠ±Π»ΠΈΠΊΠ°ΡΠΈΠΈ, ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π½ΡΠ΅ Π²ΠΎΠΏΡΠΎΡΠ°ΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΌΡΡΡΡΠΊΠ° Π² Π±ΡΡΡΡ
Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΡΡ
. Π Π°ΡΡΠΌΠΎΡΡΠ΅Π½Π° Π½ΠΎΠΌΠ΅Π½ΠΊΠ»Π°ΡΡΡΠ° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅ΠΌΡΡ
ΠΈ Π½ΠΎΡΠΌΠΈΡΡΠ΅ΠΌΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΌΡΡΡΡΠΊΠ°, Π²ΡΠ΄Π΅Π»Π΅Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΈ ΠΎΠ±ΠΎΠ·Π½Π°ΡΠ΅Π½Ρ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΠΈ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΠΏΡΠ΅Π΄Π΅Π»ΠΎΠ² ΠΈΡ
ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² ΠΠ Π. ΠΡΠΌΠ΅ΡΠ΅Π½Ρ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ Π½Π°ΠΊΠΎΠΏΠ»Π΅Π½ΠΈΡ Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΌΡΡΡΡΠΊΠ° Π±ΡΡΡΠΌΠΈ Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΡΠΌΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π² Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π΅ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΡ
ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉ Π»ΠΈΠ±ΠΎ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΠ΅ Π½ΠΎΡΠΌΡ ΠΏΠΎ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΌΡΡΡΡΠΊΠ° Π² Π±ΡΡΡΡ
Π²ΠΎΠ΄ΠΎΡΠΎΡΠ»ΡΡ
, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΠ΅ΡΡ ΠΎΡ ΠΎΠ±ΡΠΈΡ
Π½ΠΎΡΠΌ Π΄Π»Ρ ΠΠ Π, Π»ΠΈΠ±ΠΎ ΡΡΡΠ΅ΡΡΠ²ΡΡΡΠΈΠ΅ Π½ΠΎΡΠΌΡ ΡΡΠΈΡΡΠ²Π°ΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΎΠΊΡΠΈΡΠ½ΠΎΡΡΡ ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΌΡΡΡΡΠΊΠ°. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΡ Π½ΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΡΡ
ΡΠΎΠΊΡΠΈΠΊΠ°Π½ΡΠΎΠ², ΠΎΡΠ½ΠΎΠ²Π°Π½Π½Π°Ρ Π½Π° ΠΈΡ
ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΎΠΏΡΡΡΠΈΠΌΠΎΠΉ ΡΡΡΠΎΡΠ½ΠΎΠΉ Π΄ΠΎΠ·Π΅ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ. ΠΠΎΠΊΠ°Π·Π°Π½Π° Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΡ ΡΠ°Π·Π΄Π΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΎΠΊΡΠΈΡΠ½ΡΡ
Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ ΠΌΡΡΡΡΠΊΠ° ΠΈ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΠΎ ΡΠΎΠΊΡΠΈΡΠ½ΡΡ
ΠΌΠ΅ΡΠΈΠ»Π°ΡΡΠΎΠ½Π°ΡΠ° ΠΈ Π΄ΠΈΠΌΠ΅ΡΠΈΠ»Π°ΡΡΠΈΠ½Π°ΡΠ° Π² Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°Ρ
ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΠΈΠ½ΡΡΠ»ΠΈΠ½Π°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π°ΡΠΎΠΌΠ½ΠΎ-Π°Π±ΡΠΎΡΠ±ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ
Scientific relevance. Zinc quantification in insulin active substances and preparations by atomic absorption spectrometry requires a sample preparation procedure using hydrochloric acid, a narcotic and psychotropic precursor. The exclusion of precursors from laboratory practice significantly reduces labour costs during analysis.Aim. This study aimed to modify the pharmacopoeial method for quantifying zinc in insulins with a view to eliminating drug precursors and to evaluate the applicability of the modified method across different insulin preparations.Materials and methods. The study considered insulin active substances and preparations of various types, dosage forms, and strengths. Zinc content determination involved using an Agilent 240FS atomic absorption spectrometer at a characteristic wavelength of 213.86 nm.Results. The authors selected the conditions for elemental analysis (nitric acid concentration, monochromator slit width, and flame stabilisation time) corresponding to the pharmacopoeial requirements for quantifying zinc in insulins (RSDΒ β€1.4%, standard zinc solution with a concentration of 0.8 mg/dm3, and calibration curve correlation coefficientΒ β₯0.99). The study compared zinc content measurements in the studied insulin samples with hydrochloric acid or nitric acid as a solvent; it did not reveal any significant differences between the results obtained by the pharmacopoeial method and the modified method.Conclusions. The modified method enables analysts to quantify zinc in insulin preparations in various dosage forms without drug precursors.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. Π₯Π»ΠΎΡΠΎΠ²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½Π°Ρ ΠΊΠΈΡΠ»ΠΎΡΠ°, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΠ°Ρ Π½Π° ΡΡΠ°Π΄ΠΈΠΈ ΠΏΡΠΎΠ±ΠΎΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΏΡΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΡΡΠ±ΡΡΠ°Π½ΡΠΈΡΡ
ΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°Ρ
ΠΈΠ½ΡΡΠ»ΠΈΠ½Π° ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π°ΡΠΎΠΌΠ½ΠΎ-Π°Π±ΡΠΎΡΠ±ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ, ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΏΡΠ΅ΠΊΡΡΡΠΎΡΠΎΠΌ Π½Π°ΡΠΊΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π²Π΅ΡΠ΅ΡΡΠ² ΠΈ ΠΏΡΠΈΡ
ΠΎΡΡΠΎΠΏΠ½ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². ΠΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΊΡΡΡΠΎΡΠΎΠ² ΠΈΠ· Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΠΏΡΠ°ΠΊΡΠΈΠΊΠΈ Π·Π°ΠΌΠ΅ΡΠ½ΠΎ ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΡΡΡΠ΄ΠΎΠ·Π°ΡΡΠ°ΡΡ ΠΏΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π°.Π¦Π΅Π»Ρ. ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΠΈΠ½ΡΡΠ»ΠΈΠ½Π°Ρ
, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½Π°Ρ Π½Π° ΠΈΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΊΡΡΡΠΎΡΠΎΠ² Π½Π°ΡΠΊΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π²Π΅ΡΠ΅ΡΡΠ², ΠΈ ΠΎΡΠ΅Π½ΠΊΠ° ΠΏΡΠΈΠΌΠ΅Π½ΠΈΠΌΠΎΡΡΠΈ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Π΄Π»Ρ Π°Π½Π°Π»ΠΈΠ·Π° ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΈΠ½ΡΡΠ»ΠΈΠ½Π°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π€Π°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ±ΡΡΠ°Π½ΡΠΈΠΈ ΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΈΠ½ΡΡΠ»ΠΈΠ½Π° ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠΎΡΠΌ ΠΈ Π΄ΠΎΠ·ΠΈΡΠΎΠ²ΠΎΠΊ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° Π°ΡΠΎΠΌΠ½ΠΎ-Π°Π±ΡΠΎΡΠ±ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠ΅ Agilent 240FS ΠΏΡΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄Π»ΠΈΠ½Π΅ Π²ΠΎΠ»Π½Ρ 213,86Β Π½ΠΌ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΠ΄ΠΎΠ±ΡΠ°Π½Ρ ΡΡΠ»ΠΎΠ²ΠΈΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ Π°Π·ΠΎΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ, ΡΠΈΡΠΈΠ½Π° ΡΠ΅Π»ΠΈ ΠΌΠΎΠ½ΠΎΡ
ΡΠΎΠΌΠ°ΡΠΎΡΠ°, Π²ΡΠ΅ΠΌΡ ΡΡΠ°Π±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΠ»Π°ΠΌΠ΅Π½ΠΈ), ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠ΅ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠ΅ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΡΠΌ ΡΡΠ΅Π±ΠΎΠ²Π°Π½ΠΈΡΠΌ ΠΊ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΠΈΠ½ΡΡΠ»ΠΈΠ½Π°Ρ
(RSDβ€1,4% Π΄Π»Ρ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΡΡΠ²ΠΎΡΠ° ΡΠΈΠ½ΠΊΠ° Ρ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠ΅ΠΉ 0,8 ΠΌΠ³/Π΄ΠΌ3, ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ ΠΊΠ°Π»ΠΈΠ±ΡΠΎΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ Π³ΡΠ°ΡΠΈΠΊΠ° Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 0,99). ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΈΠ·ΠΌΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠ°ΡΡΠ²ΠΎΡΠΈΡΠ΅Π»Ρ Ρ
Π»ΠΎΡΠΎΠ²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ»ΠΈ Π°Π·ΠΎΡΠ½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ. Π‘ΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ Ρ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΠΎΠΉ Π½Π΅ Π²ΡΡΠ²ΠΈΠ»ΠΎ ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠ°ΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ°Ρ
ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° ΠΏΡΠΈ Π·Π°ΠΌΠ΅Π½Π΅ Ρ
Π»ΠΎΡΠΎΠ²ΠΎΠ΄ΠΎΡΠΎΠ΄Π½ΠΎΠΉ ΠΊΠΈΡΠ»ΠΎΡΡ Π½Π° Π°Π·ΠΎΡΠ½ΡΡ.ΠΡΠ²ΠΎΠ΄Ρ. ΠΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡ Π°Π½Π°Π»ΠΈΠ· ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΠ½ΠΊΠ° Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΠΎΡΠΌΠ°Ρ
ΠΈΠ½ΡΡΠ»ΠΈΠ½Π° Π±Π΅Π· ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠ΅ΠΊΡΡΡΠΎΡΠΎΠ² Π½Π°ΡΠΊΠΎΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π²Π΅ΡΠ΅ΡΡΠ²
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