Surface topography of the InSb-MnSb thin films

In that report we observe a semiconductor eutectic composite InSb-MnSb thin films, prepared by the "flash evaporation" method. The atomic force microscopy and the scanning electron microscopy were employed for investigation microstructure and surface relief of the InSb-MnSb thin films. В этом отчете мы наблюдаем за тонкими пленками полупроводникового электического композита InSb-MnSb, полученными методом «мгновенного испарения». С помощью атомно-силовой микроскопии и сканирующей электронной микроскопии исследованы микроструктура и рельеф поверхности тонких пленок InSb-MnSb

Surface topography of the InSb-MnSb thin films

In that report we observe a semiconductor eutectic composite InSb-MnSb thin films, prepared by the "flash evaporation" method. The atomic force microscopy and the scanning electron microscopy were employed for investigation microstructure and surface relief of the InSb-MnSb thin films. В этом отчете мы наблюдаем за тонкими пленками полупроводникового электического композита InSb-MnSb, полученными методом «мгновенного испарения». С помощью атомно-силовой микроскопии и сканирующей электронной микроскопии исследованы микроструктура и рельеф поверхности тонких пленок InSb-MnSb

Macrophage population state and proliferative activity of spleen cells under liver regeneration conditions

Relevance. Currently, the participation of immune system cells in the regulation of reparative processes is attracting more and more attention of researchers. There is an anatomical connection between the liver and spleen by means of portal vein. Thus, cytokines and other biologically active substances can enter the liver from the spleen through the portal vein, as well as cells can migrate to the liver. However, the specific mechanisms of mutual influence of the mentioned organs, including in reparative processes, remain poorly studied. The aim of our work was to study the state of spleen monocyte-macrophage population after liver resection, as well as the proliferative activity of spleen cells during liver regeneration . Materials and Methods . The model of liver regeneration after 70 % resection in mouse was reproduced in this work. The animals were taken out of the experiment after 1, 3 and 7 days. The marker of cell proliferation Ki67 was immunohistochemically detected, the state of spleen monocyte-macrophage population was evaluated by markers CD68, CD115, CD206, F4/80 by methods of immunohistochemistry and flow cytometry. Results and Discussion . The liver regeneration had a pronounced effect on the cytoarchitectonics of the spleen. In 1 day after liver resection in the spleen there was observed a decrease in the share of Ki67+cells, according to the flow cytometry data there was a decrease in the number of CD115+cells, in 3 and 7 days there was a decrease in the number of F4/80+ macrophages. Conclusion . Liver resection causes changes in the state of cell populations of the spleen as well. First of all, to the decrease in the activity of proliferative processes in it, as well as to the changes in the state of the monocyte-macrophage system. A decrease in the content of CD115+ and F4/80+ cells in the spleen was found, which indirectly indicates the migration of monocytes/macrophages after liver resection, which can also influence the course of reparative processes in the liver

One-step isolation and biochemical characterization of a highlyactive plant PSII monomeric core

We describe a one-step detergent solubilization protocol for isolating a highly active form of Photosystem II (PSII) from Pisum sativum L. Detailed characterization of the preparation showed that the complex was a monomer having no light harvesting proteins attached. This core reaction centre complex had, however, a range of low molecular mass intrinsic proteins as well as the chlorophyll binding proteins CP43 and CP47 and the reaction centre proteins D1 and D2. Of particular note was the presence of a stoichiometric level of PsbW, a low molecular weight protein not present in PSII of cyanobacteria. Despite the high oxygen evolution rate, the core complex did not retain the PsbQ extrinsic protein although there was close to a full complement of PsbO and PsbR and partial level of PsbP. However, reconstitution of PsbP and PsbPQ was possible. The presence of PsbP in absence of LHCII and other chlorophyll a/b binding proteins confirms that LHCII proteins are not a strict requirement for the assembly of this extrinsic polypeptide to the PSII core in contrast with the conclusion of Caffarri et al. (2009)

ХОНДРОСАРКОМА ЛОННОЙ КОСТИ С ПРОРАСТАНИЕМ В МОЧЕВОЙ ПУЗЫРЬ (СЛУЧАЙ ИЗ ПРАКТИКИ)

The paper describes a clinical case of pubic chondrosarcoma invading the bladder. Radical cystectomy has been performed with resection of the pubic symphysis and a part of the left ischium.Представлено клиническое наблюдение хондросаркомы лонной кости, прорастающей в мочевой пузырь. Проведена радикальная цистэктомия с резекцией лонного сочленения и части левой седалищной кости

Тридцатидневные осложнения радикальной цистэктомии и факторы, влияющие на их развитие

Objective: to identify the most important factors influencing the development of complications after radical cystectomy (RCE).Materials and methods. The results of 182 RCEs performed in September 2014 and December 2016 were analyzed. The patients included 152 (83.5 %) men and 30 (16.5 %) women. The patients’ age ranged from 31 to 84 years (mean age 62.3 years). An orthotopic urinary reservoir was formed in 138 (75.8 %) patients. Postoperative complications were evaluated according to the Clavien–Dindo classification. The calculations were made with a computer program for SPSS Statistics 16.Results. No complications were recorded in 84 (46.2 %) patients within 30 days after RCE. The development of various postoperative adverse reactions was observed in 98 (53.8 %) patients. The most common complications were gastrointestinal (26.9 %) and infectious (25.8 %). Sixteen (8.8 %) patients developed grade I complications; grades II, III, IV, and V complications were seen in 53 (29.1 %), 21 (11.5 %), 5 (2.7 %), in 3 patients, respectively. Thirty-day mortality was 1.64 %. Univariate regression analysis has established that overweight (p = 0.031), T status (p = 0.021), preoperative hemoglobin levels (p = 0.001), intraoperative blood loss (p = 0.009), and intraoperative abdominal cavity infection (p <0.001) play a significant role in the development of complications following RCE. Multivariate regression analysis has revealed that the frequency of complications after RCE is influenced by body mass index (p = 0.008), preoperative anemia (p = 0.034), blood loss (p = 0.003), and intraoperative abdominal cavity infection (p <0.001).Conclusion. RCE is often accompanied by the development of different categories and severity of complications. Identification of risk factors for their complications will be able to elaborate effective ways for their prevention.Цель исследования – выявление наиболее значимых факторов, влияющих на развитие осложнений после проведения радикальной цистэктомии (РЦЭ).Материалы и методы. Проанализированы результаты 182 РЦЭ, произведенных с сентября 2014 г. по декабрь 2016 г. Среди пациентов были 152 (83,5 %) мужчин и 30 (16,5 %) женщин. Возраст больных варьировал от 31 до 84 лет (средний возраст 62,3 года). У 138 (75,8 %) пациентов сформирован ортотопический мочевой резервуар. Послеоперационные осложнения оценивали по классификации Clavien–Dindo. Расчеты выполняли в компьютерной программе для статистической обработки данных SPSS Statistics 16.Результаты. У 84 (46,2 %) пациентов в 30-дневные сроки после РЦЭ осложнений не зарегистрировано. У 98 (53,8 %) больных было зафиксировано развитие различных нежелательных отклонений после операции. Наиболее частыми осложнениями явились желудочно-кишечные (26,9 %) и инфекционные (25,8 %). У 16 (8,8 %) пациентов развились осложнения I степени, у 53 (29,1 %) – II, у 21 (11,5 %) – III, у 5 (2,7 %) – IV, у 3 пациентов – V. Тридцатидневная летальность составила 1,64 %. Однофакторный регрессионный анализ установил значимую роль избыточной массы тела больных (p = 0,031), T-статуса опухоли (p = 0,021), уровня гемоглобина до операции (p = 0,001), объема интраоперационной кровопотери (р = 0,009) и интраоперационного инфицирования брюшной полости (р <0,001) в развитии осложнений после РЦЭ. Многофакторный регрессионный анализ выявил, что на частоту развития осложнений после РЦЭ влияют индекс массы тела (р = 0,008), анемия до операции (р = 0,034), кровопотеря (р = 0,003) и интраоперационное инфицирование брюшной полости (р <0,001).Заключение. РЦЭ часто сопровождается развитием осложнений различных категорий и степени тяжести. Выявление факторов риска их развития позволит разработать эффективные пути их профилактики

Importance of immunotherapy in the treatment of recurrent cystitis

The review is devoted to the problems of diagnosis and treatment of chronic recurrent cystitis (CRC). The search was conducted using the Medline, PubMed, EMBASE databases. CRC is a common disease, especially among women of reproductive age. Human papillomaviruses (HPV) are part of the human virome and may be implicated in the development of CRC. Immunostimulating therapy is an effective alternative method for the treatment and prevention of CRC, notably in CRC of human papillomavirus aetiology

The immunological aspects of male infertility: 2016 - 2020 literature review

Approximately 15% of sexually active couples without using various methods of contraception do not achieve pregnancy within a year. At the same time, the cause of infertility is not identified in 30-40% of cases, which indicates the urgency of this problem and many existing questions regarding the developmental mechanisms that can lead to infertility. Many mechanisms of the development of infertility remain unclear, and therefore it is often impossible to carry out adequate diagnosis and treatment. The purpose of the review was to highlight the accumulated material on the topic of male immune infertility, to determine the direction of further research. Our review confirmed the urgent need for a detailed study of the mechanisms of immunological infertility. Emphasis is made on the areas on which further research to study this problem should be based