5 research outputs found
EpsteinβBarr virus LMP1 oncogene polymorphism in tatar and slavic populations in Russian Federation impacting on some malignant tumours
Objective: To compare genetic structure of the main EpsteinβBarr virus (EBV) oncogene, latent membrane protein 1 (LMP1), in EBV strains circulating in two genetically distinct ethnic populations in Russian Federation, Tatars and Slavs, as well as assess an impact of diverse LMP1 variants on incidence and mortality rate for some malignant tumors partially associated with EBV infection. Materials and methods. Oral washing samples were collected from 60 ethnic Kazan Tatars and 65 ethnic Moscow Slavics. Carboxy-terminal nucleotide sequences (41 and 40 sequences, respectively) derived from hypervariable LMP1 gene region were amplified from EBV DNA samples. Next, final nucleotide sequences were translated into amino acid sequences and analyzed according to classification by Edwards et al. Results. Analysis of 41 and 40 LMP1 samples obtained from ethnic Kasan Tatars and ethnic Moscow Slavics, respectively, revealed significant difference in relevant amino acid structures. In particular, all LMP1 samples derived from Moscow Slavics were found to belong to the four protein variants: B95.8/A, Medβ, China1 and NC. Among them, low-transforming variant B95.8/A was dominant (82.5%). In contrast, solely 21 out of 41 LMP1 samples derived from ethnic Tatars were classified as B95.8/A, Medβ and China1 variants. Importantly, the percentage of low-transforming B95.8/A variant among ethnic Tatar samples was significantly lower compared to that one found in Moscow Slavics (29.3% vs. 82.5%). On the other hand, seven (17.1%) out of 20 other samples formed a unique protein mono group characterized by LMP1 amino acid sequence differed from that one available in the GenBank database. Such group of variants was designated as LMP1-TatK. The remaining 13 samples (31.7%) did not match either protein variants, thereby forming the βbeyond classificationβ (LMP1-TatBC) group. Conclusion. The data obtained suggest that various LMP1 variants exist in EBV strains persisting in ethnic Tatrs and ethnic Slavics examined in Russian Federation. It was also found that EBV strains isolated from ethnic Tatars contained a unique LMP1 gene variant encoding protein LMP1-TatK lacked in EBV strains derived from ethnic Moscow Slavics. Taking into account the genealogy of Tatars, it cannot be ruled out that EBV strain bearing LMP1-TatK variant represented ethnically specific EBV strain that might circulate many centuries ago among their historical human predecessors called Mongol-Tatar tribes. In addition, it was shown that the LMP1 variants in EBV strains isolated from ethnic Kazan Tatars and ethnic Moscow Slavics did not affect the incidence and mortality of different forms of cancer consisting of EBV-associated cases
ΠΠΈΡΡΡ ΠΠΏΡΡΠ΅ΠΉΠ½Π°-ΠΠ°ΡΡ Ρ Π°Π΄ΡΠ³Π΅ΠΉΡΠ΅Π² ΠΈ ΡΠ»Π°Π²ΡΠ½ Π² Π ΠΎΡΡΠΈΠΈ: ΡΠΈΠΏΡ Π²ΠΈΡΡΡΠ°, Π²Π°ΡΠΈΠ°Π½ΡΡ LMP1 ΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ
Introduction. It is known that the structural features of the Epstein-Barr virus (EBV) affect the manifestation of its biological properties. Based on differences in the sequences of the EBNA2, EBNA3A, -B, and -C genes, two types of the virus, EBV-1 and EBV-2, have been identified that have different ability to transform B cells in vitro and possibly playing certain role in the development of EBV-associated neoplasms.Aim. To study the prevalence of EBV-1 and EBV-2 in two ethnic groups, Πdygeans and Slavs, as well as the contribution of EBV-associated tumors to the overall incidence of malignant neoplasms certain organs and tissues.Materials and methods. DNA samples were extracted from 59 oral lavages of ethnic Πdygeans from Republic of Adygea and 40 such from oral cavity of ethnic Slavs of Moscow city. These samples were used for amplification of EBV DNA, determination of the concentration of viral DNA copies per 1 cell washout, as well as for amplification of EBV LMP1 followed by sequencing of the resulting gene samples and determination of their protein variant (LMP1).Results. Studies have shown that among the representatives of the Πdygeans the 2nd EBV type prevails, and among the Slavs, the 1st one. Epstein-Barr virus isolates in representatives of the two ethnic groups also differed in the structure of LMP1. Among the Slavs, a set of its LMP1 variants (B95.8/A, China, Med- and NC) was identified. However, among the Adygeans, the only variant - B95.8 and its subtype - B95.8/A was identified. EBV-1, which prevails among the representatives of the Slavs and has the ability to transform B-cells, was projected onto a higher incidence of tumors of the pharynx, stomach, Hodgkin's and non-Hodgkin's lymphomas (where EBV-associated cases cam occur) in the population of Moscow than in the population of the Republic of Adygea. However, the differences between incidence rates for these neoplasms (with the exception for the stomach tumors) were not statistically significant (p >0.5). A higher and statistically significantly different incidence rate of stomach cancer in residents of Moscow city, compared with that in residents of the Republic of Adygea, in our opinion, is not due to EBV-1 type and/or LMP1 variants, but rather is associated with a genetic predisposition the population of Moscow city to this tumor.Conclusion. The fact that two ethnic groups of Russia were found to be prevails by different types of EBV raises the question of their ethno-geographical association and their role in the induction of EBV-associated tumors. To resolve this issue additional studies in other geographical regions of Russia among representatives of different ethnic groups are required.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠ·Π²Π΅ΡΡΠ½ΠΎ, ΡΡΠΎ ΡΡΡΡΠΊΡΡΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ Π²ΠΈΡΡΡΠ° ΠΠΏΡΡΠ΅ΠΉΠ½Π°-ΠΠ°ΡΡ (ΠΠΠ) Π²Π»ΠΈΡΡΡ Π½Π° ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΠ΅ Π΅Π³ΠΎ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ². ΠΠ° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π² ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ
Π³Π΅Π½ΠΎΠ² EBNA2, EBNA3A, -B ΠΈ -C ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ 2 ΡΠΈΠΏΠ° Π²ΠΈΡΡΡΠ°, 1-ΠΉ (ΠΠΠ-1) ΠΈ 2-ΠΉ (ΠΠΠ-2), ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ ΡΠ°Π·Π½ΠΎΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡΡ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°ΡΡ Π-ΠΊΠ»Π΅ΡΠΊΠΈ in vitro ΠΈ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ, ΠΈΠ³ΡΠ°ΡΡΠΈΠ΅ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΡ ΡΠΎΠ»Ρ Π² Π²ΠΎΠ·Π½ΠΈΠΊΠ½ΠΎΠ²Π΅Π½ΠΈΠΈ ΠΠΠ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ - ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΠΈ ΠΠΠ-1 ΠΈ Π²ΠΠ-2 Ρ 2 ΡΡΠ½ΠΎΡΠΎΠ², Π°Π΄ΡΠ³Π΅ΠΉΡΠ΅Π² ΠΈ ΡΠ»Π°Π²ΡΠ½, Π° ΡΠ°ΠΊΠΆΠ΅ Π²ΠΊΠ»Π°Π΄Π° Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Ρ ΠΠΠ ΡΠ»ΡΡΠ°Π΅Π² Π² ΠΎΠ±ΡΡΡ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΡ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡΠΌΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΡ
ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΈ ΡΠΊΠ°Π½Π΅ΠΉ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ· 59 ΡΠΌΡΠ²ΠΎΠ² ΠΏΠΎΠ»ΠΎΡΡΠΈ ΡΡΠ° ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
Π°Π΄ΡΠ³Π΅ΠΉΡΠ΅Π² Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠΈ ΠΠ΄ΡΠ³Π΅Ρ ΠΈ 40 ΡΠ°ΠΊΠΎΠ²ΡΡ
ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ»Π°Π²ΡΠ½ ΠΠΎΡΠΊΠ²Ρ ΡΠΊΡΡΡΠ°Π³ΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΠΠ. ΠΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΠΠ ΠΠΠ, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΊΠΎΠΏΠΈΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΠΠ Π½Π° 1 ΠΊΠ»Π΅ΡΠΊΡ ΡΠΌΡΠ²Π°, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ LMP1 ΠΠΠ Ρ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠΈΠΌ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π³Π΅Π½Π° ΠΈ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠ³ΠΎ Π²Π°ΡΠΈΠ°Π½ΡΠ° (LMP1).Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ, ΡΡΠΎ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ Π°Π΄ΡΠ³Π΅ΠΉΡΠ΅Π² ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΠΠΠ-2, Π° Ρ ΡΠ»Π°Π²ΡΠ½ - ΠΠΠ-1. ΠΈΠ·ΠΎΠ»ΡΡΡ ΠΠΠ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ 2 ΡΡΠ½ΠΎΡΠΎΠ² ΡΠ°ΠΊΠΆΠ΅ ΡΠ°Π·Π»ΠΈΡΠ°Π»ΠΈΡΡ ΠΏΠΎ ΡΡΡΡΠΊΡΡΡΠ΅ Π΅Π³ΠΎ LMP1: Ρ ΡΠ»Π°Π²ΡΠ½ Π²ΡΡΠ²Π»Π΅Π½ ΡΠ΅Π»ΡΠΉ Π½Π°Π±ΠΎΡ Π΅Π³ΠΎ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² ( Π95.8/Π, China, Med- ΠΈ NC), Π° Ρ Π°Π΄ΡΠ³Π΅ΠΉΡΠ΅Π² - Π΅Π΄ΠΈΠ½ΡΡΠ²Π΅Π½Π½ΡΠΉ Π²Π°ΡΠΈΠ°Π½Ρ - B95.8 ΠΈ Π΅Π³ΠΎ ΠΏΠΎΠ΄ΡΠΈΠΏ -B95.8/A. Π΄ΠΎΠΌΠΈΠ½ΠΈΡΡΡΡΠΈΠΉ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΡΠ»Π°Π²ΡΠ½ ΠΠΠ-1, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΉ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΡΡ ΡΡΠ°Π½ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°ΡΡ Π²-ΠΊΠ»Π΅ΡΠΊΠΈ, ΠΏΡΠΎΠ΅ΡΠΈΡΠΎΠ²Π°Π»ΡΡ Π½Π° Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΡΡ Ρ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΠΎΡΠΊΠ²Ρ, ΡΠ΅ΠΌ Ρ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠΈ ΠΠ΄ΡΠ³Π΅Ρ, Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ Π³Π»ΠΎΡΠΊΠΈ, ΠΆΠ΅Π»ΡΠ΄ΠΊΠ°, Π»ΠΈΠΌΡΠΎΠΌΠΎΠΉ Π₯ΠΎΠ΄ΠΆΠΊΠΈΠ½Π° ΠΈ Π½Π΅Ρ
ΠΎΠ΄ΠΆΠΊΠΈΠ½ΡΠΊΠΈΠΌΠΈ Π»ΠΈΠΌΡΠΎΠΌΠ°ΠΌΠΈ, Π² ΠΊΠΎΡΠΎΡΡΡ
Π²ΡΡΡΠ΅ΡΠ°ΡΡΡΡ ΠΠΠ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΠ»ΡΡΠ°ΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΠΈ Π΄Π»Ρ ΡΠΊΠ°Π·Π°Π½Π½ΡΡ
ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΉ (Π·Π° ΠΈΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π°Π½Π½ΡΡ
Π΄Π»Ρ ΠΆΠ΅Π»ΡΠ΄ΠΊΠ°) Π±ΡΠ»ΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠΌΠΈ (p >0,5). ΠΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠΉ ΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΠΉΡΡ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΠΈ ΡΠ°ΠΊΠΎΠΌ ΠΆΠ΅Π»ΡΠ΄ΠΊΠ° Ρ ΠΆΠΈΡΠ΅Π»Π΅ΠΉ ΠΠΎΡΠΊΠ²Ρ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΡΠ°ΠΊΠΎΠ²ΡΠΌ Ρ ΠΆΠΈΡΠ΅Π»Π΅ΠΉ Π Π΅ΡΠΏΡΠ±Π»ΠΈΠΊΠΈ ΠΠ΄ΡΠ³Π΅Ρ, ΠΏΠΎ Π½Π°ΡΠ΅ΠΌΡ ΠΌΠ½Π΅Π½ΠΈΡ, Π½Π΅ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ ΠΠΠ-1 ΠΈ/ΠΈΠ»ΠΈ Π²Π°ΡΠΈΠ°Π½ΡΠ°ΠΌΠΈ LMP1, Π° ΡΠΊΠΎΡΠ΅Π΅ ΡΠ²ΡΠ·Π°Π½ Ρ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠ΅Π΄ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΡΡΡΡ ΠΊ ΡΡΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΠΎΡΠΊΠ²Ρ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π€Π°ΠΊΡ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΡ Ρ 2 ΡΡΠ½ΠΎΡΠΎΠ² Π ΠΎΡΡΠΈΠΈ ΠΏΡΠ΅Π²Π°Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΈΠΏΠΎΠ² ΠΠΠ ΠΏΠΎΠ΄Π½ΠΈΠΌΠ°Π΅Ρ Π²ΠΎΠΏΡΠΎΡ ΠΎΠ± ΠΈΡ
ΡΡΠ½ΠΎΠ³Π΅ΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΠΈ ΡΠΎΠ»ΠΈ Π² ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ ΠΠΠ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠΉ. Π΄Π»Ρ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΡΠΎΠ³ΠΎ Π²ΠΎΠΏΡΠΎΡΠ° Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π² Π΄ΡΡΠ³ΠΈΡ
Π³Π΅ΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Π³ΠΈΠΎΠ½Π°Ρ
Π ΠΎΡΡΠΈΠΈ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΡΠ°Π·Π½ΡΡ
ΡΡΠ½ΠΎΡΠΎΠ²
ΠΠΈΡΡΡ ΠΠΏΡΡΠ΅ΠΉΠ½Π°βΠΠ°ΡΡ Ρ ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ ΡΠ°ΡΠ°Ρ: ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΡΡΡ ΠΈ ΡΠΈΠΊΠ²Π΅Π½ΡΠ½ΡΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΡ ΠΎΠ½ΠΊΠΎΠ³Π΅Π½Π° LMP1
Objective of the investigation was to study the infection of ethnic Tatars with the EpsteinβBarr virus (EBV) and to analyze the genetic structure of the oncogene of the virus, the latent membrane protein 1 (LMP1), in the virus strains of Tatar origin. Materials and methods. The materials for the study were samples of boucle flushes of 60 students from the Kazan State Medical University who are ethnic Tatars (Tatars no less than in the 3rd generation). Amplified from DNA of boucle flushes the nucleotide sequences of the LMP1 samples translated into DNA amino acid sequences, have undergone classification based on the well-known and widely used in literature the R.H. Edwards et al. classification. Results. The analysis of nucleotide and deductive amino acid sequences of the 41 LMP1 amplicons revealed their homology with only three gene variants from the R.H. Edwards et al. classification (1999): 95.8/A (29.3 %; 12/41), Medβ (14.6 %; 6/41) and China1 (7.3 %, 3/41).Β Such variants of LMP1 as Alaskan, Med+, ChinΠ°2, China3 and NC, were not found. Among the LMP1 samples of Tatar origin in 20 cases (48.8 %), the composition of the mutations found did not allow them to be assigned to any of the oncogene variants listed above. Out of this number, in 7 (17.1 %) cases a mono group of LMP1 samples was found, differing not only from representatives of the Slavs, inhabitants of the European part of Russia, but also from other Kazan samples, and was designated as LMP1-TatK. The remaining 13 samples of LMP1 (31.7 %), not belonging to any of the known classifications, formed the group designated by us as an LMP1 group beside the classification (LMP1BC). Conclusion. Continuation of the study of the molecular-biological and functional properties of LMP1 in TatK and BC groups, which constitute 48.8 % of the number of gene samples studied, and an analysis of the peculiarities of the ethnic Tatar genotype, will probably help to clarify whether certain EBV strains influence morbidity and mortality in Tatar population with malignant neoplasms, which include EBVassociated cases.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΡΡΠΈ Π²ΠΈΡΡΡΠΎΠΌ ΠΠΏΡΡΠ΅ΠΉΠ½Π°βΠΠ°ΡΡ (ΠΠΠ) ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΡΠ°Ρ ΠΈ Π°Π½Π°Π»ΠΈΠ· Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ ΠΎΠ½ΠΊΠΎΠ³Π΅Π½Π° Π²ΠΈΡΡΡΠ°, Π»Π°ΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π½ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° 1 (LMP1), Π² ΡΡΠ°ΠΌΠΌΠ°Ρ
Π²ΠΈΡΡΡΠ° ΡΠ°ΡΠ°ΡΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ Π΄Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ»ΡΠΆΠΈΠ»ΠΈ Π±ΡΠΊΠΊΠ°Π»ΡΠ½ΡΠ΅ ΡΠΌΡΠ²Ρ 60 ΡΡΡΠ΄Π΅Π½ΡΠΎΠ² ΠΠ°Π·Π°Π½ΡΠΊΠΎΠ³ΠΎ Π³ΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΎΠ³ΠΎ ΡΠ½ΠΈΠ²Π΅ΡΡΠΈΡΠ΅ΡΠ°, ΡΠ²Π»ΡΡΡΠΈΡ
ΡΡ ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠ°ΡΠ°ΡΠ°ΠΌΠΈ (Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ ΡΠ΅ΠΌ Π² III ΠΏΠΎΠΊΠΎΠ»Π΅Π½ΠΈΠΈ). ΠΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ ΠΈΠ· ΡΠΌΡΠ²ΠΎΠ² ΠΠΠ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈ LMP1. ΠΠΌΠΏΠ»ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ· ΠΠΠ Π±ΡΠΊΠΊΠ°Π»ΡΠ½ΡΡ
ΡΠΌΡΠ²ΠΎΠ² Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΠ΅ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² LMP1, ΡΡΠ°Π½ΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π² Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΠ΅ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π»ΠΈΡΡ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎΠΉ ΠΈ ΡΠΈΡΠΎΠΊΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΠΎΠΉ Π² Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ R.H. Edwards ΠΈ ΡΠΎΠ°Π²Ρ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ½Π°Π»ΠΈΠ· Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ
ΠΈ ΡΡΠ°Π½ΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π°ΠΌΠΈΠ½ΠΎΠΊΠΈΡΠ»ΠΎΡΠ½ΡΡ
ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠ΅ΠΉ 41-Π³ΠΎ Π°ΠΌΠΏΠ»ΠΈΠΊΠΎΠ½Π° LMP1 Π²ΡΡΠ²ΠΈΠ» ΠΈΡ
Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΡ ΡΠΎΠ»ΡΠΊΠΎ Ρ 3 Π²Π°ΡΠΈΠ°Π½ΡΠ°ΠΌΠΈ Π³Π΅Π½Π° ΠΈΠ· ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ R.H. Edwards ΠΈ ΡΠΎΠ°Π²Ρ.: 95.8/Π (29,3 %; 12/41), Medβ (14,6 %; 6/41) ΠΈ China1 (7,3 %; 3/41). Π’Π°ΠΊΠΈΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΡ LMP1, ΠΊΠ°ΠΊ Alaskan, Med+, ChinΠ°2, China3 ΠΈ NC, Π½Π΅ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Ρ. Π ΠΎΡΡΠ°Π»ΡΠ½ΡΡ
20 ΡΠ»ΡΡΠ°ΡΡ
(48,8 %) ΡΠΏΠ΅ΠΊΡΡ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π½ΡΡ
ΠΌΡΡΠ°ΡΠΈΠΉ Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
LMP1 ΡΠ°ΡΠ°ΡΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π½Π΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΠ» ΠΈΡ
ΠΎΡΠ½Π΅ΡΡΠΈ Π½ΠΈ ΠΊ ΠΎΠ΄Π½ΠΎΠΌΡ ΠΈΠ· ΠΏΠ΅ΡΠ΅ΡΠΈΡΠ»Π΅Π½Π½ΡΡ
Π²ΡΡΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² ΠΎΠ½ΠΊΠΎΠ³Π΅Π½Π°. ΠΠ· Π½ΠΈΡ
Π² 7 ΡΠ»ΡΡΠ°ΡΡ
(17,1 % Π²ΡΠ΅Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ²) ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π° ΠΌΠΎΠ½ΠΎΠ³ΡΡΠΏΠΏΠ° Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² LMP1, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠ°ΡΡΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ ΠΎΡ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΡΠ»Π°Π²ΡΠ½, ΠΆΠΈΡΠ΅Π»Π΅ΠΉ Π΅Π²ΡΠΎΠΏΠ΅ΠΉΡΠΊΠΎΠΉ ΡΠ°ΡΡΠΈ Π ΠΎΡΡΠΈΠΈ, Π½ΠΎ ΠΈ ΠΎΡ Π΄ΡΡΠ³ΠΈΡ
ΠΊΠ°Π·Π°Π½ΡΠΊΠΈΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ², ΠΈ ΠΎΠ±ΠΎΠ·Π½Π°ΡΠ΅Π½Π½Π°Ρ Π½Π°ΠΌΠΈ, ΠΊΠ°ΠΊ LMP1-TatK. ΠΡΡΠ°Π»ΡΠ½ΡΠ΅ 13 ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² LMP1 (31,7 %), Π½Π΅ ΠΎΡΠ½ΠΎΡΡΡΠΈΡ
ΡΡ Π½ΠΈ ΠΊ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΈΠ·Π²Π΅ΡΡΠ½ΡΡ
ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΉ, ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π»ΠΈ Π³ΡΡΠΏΠΏΡ, ΠΎΠ±ΠΎΠ·Π½Π°ΡΠ΅Π½Π½ΡΡ Π½Π°ΠΌΠΈ, ΠΊΠ°ΠΊ Π³ΡΡΠΏΠΏΠ° LMP1 Π²Π½Π΅ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ (LMP1ΠΠ). ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠ°Π»ΡΠ½Π΅ΠΉΡΠ΅Π΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΡΠ²ΠΎΠΉΡΡΠ² LMP1 Π² Π³ΡΡΠΏΠΏΠ°Ρ
ΠΠ ΠΈ TatK, ΡΠΎΡΡΠ°Π²Π»ΡΡΡΠΈΡ
48,8 % ΠΎΡ ΡΠΈΡΠ»Π° ΠΈΠ·ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΠΎΠ½ΠΊΠΎΠ±Π΅Π»ΠΊΠ°, ΠΈ Π°Π½Π°Π»ΠΈΠ· ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΡΠ°Ρ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ Π²ΡΡΡΠ½ΠΈΡΡ, ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ Π»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠ΅ ΡΡΠ°ΠΌΠΌΡ ΠΠΠ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π½Π° ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π΅ΠΌΠΎΡΡΠΈ ΠΈ ΡΠΌΠ΅ΡΡΠ½ΠΎΡΡΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ Π½ΠΎΠ²ΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡΠΌΠΈ, Π² ΡΠΎΡΡΠ°Π² ΠΊΠΎΡΠΎΡΡΡ
Π²Ρ
ΠΎΠ΄ΡΡ ΠΠΠ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΠ»ΡΡΠ°ΠΈ, Ρ ΡΠ°ΡΠ°ΡΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ
EpsteinβBarr virus in the ethnic Tatars population: the infection and sequence variants of LMP1 oncogene
Objective of the investigation was to study the infection of ethnic Tatars with the EpsteinβBarr virus (EBV) and to analyze the genetic structure of the oncogene of the virus, the latent membrane protein 1 (LMP1), in the virus strains of Tatar origin. Materials and methods. The materials for the study were samples of boucle flushes of 60 students from the Kazan State Medical University who are ethnic Tatars (Tatars no less than in the 3rd generation). Amplified from DNA of boucle flushes the nucleotide sequences of the LMP1 samples translated into DNA amino acid sequences, have undergone classification based on the well-known and widely used in literature the R.H. Edwards et al. classification. Results. The analysis of nucleotide and deductive amino acid sequences of the 41 LMP1 amplicons revealed their homology with only three gene variants from the R.H. Edwards et al. classification (1999): 95.8/A (29.3 %; 12/41), Medβ (14.6 %; 6/41) and China1 (7.3 %, 3/41).Β Such variants of LMP1 as Alaskan, Med+, ChinΠ°2, China3 and NC, were not found. Among the LMP1 samples of Tatar origin in 20 cases (48.8 %), the composition of the mutations found did not allow them to be assigned to any of the oncogene variants listed above. Out of this number, in 7 (17.1 %) cases a mono group of LMP1 samples was found, differing not only from representatives of the Slavs, inhabitants of the European part of Russia, but also from other Kazan samples, and was designated as LMP1-TatK. The remaining 13 samples of LMP1 (31.7 %), not belonging to any of the known classifications, formed the group designated by us as an LMP1 group beside the classification (LMP1BC). Conclusion. Continuation of the study of the molecular-biological and functional properties of LMP1 in TatK and BC groups, which constitute 48.8 % of the number of gene samples studied, and an analysis of the peculiarities of the ethnic Tatar genotype, will probably help to clarify whether certain EBV strains influence morbidity and mortality in Tatar population with malignant neoplasms, which include EBVassociated cases