1,583 research outputs found
Building Proteins in a Day: Efficient 3D Molecular Reconstruction
Discovering the 3D atomic structure of molecules such as proteins and viruses
is a fundamental research problem in biology and medicine. Electron
Cryomicroscopy (Cryo-EM) is a promising vision-based technique for structure
estimation which attempts to reconstruct 3D structures from 2D images. This
paper addresses the challenging problem of 3D reconstruction from 2D Cryo-EM
images. A new framework for estimation is introduced which relies on modern
stochastic optimization techniques to scale to large datasets. We also
introduce a novel technique which reduces the cost of evaluating the objective
function during optimization by over five orders or magnitude. The net result
is an approach capable of estimating 3D molecular structure from large scale
datasets in about a day on a single workstation.Comment: To be presented at IEEE Conference on Computer Vision and Pattern
Recognition (CVPR) 201
Multiple Time Scales in Diffraction Measurements of Diffusive Surface Relaxation
We grew SrTiO3 on SrTiO3 (001) by pulsed laser deposition, using x-ray
scattering to monitor the growth in real time. The time-resolved small angle
scattering exhibits a well-defined length scale associated with the spacing
between unit cell high surface features. This length scale imposes a discrete
spectrum of Fourier components and rate constants upon the diffusion equation
solution, evident in multiple exponential relaxation of the "anti-Bragg"
diffracted intensity. An Arrhenius analysis of measured rate constants confirms
that they originate from a single activation energy.Comment: 4 pages, 3 figure
Spatial oscillations in the spontaneous emission rate of an atom inside a metallic wedge
A method of images is applied to study the spontaneous emission of an atom
inside a metallic wedge with an opening angle of , where N is an
arbitrary positive integer. We show the method of images gives a rate formula
consistent with that from Quantum Electrodynamics. Using the method of images,
we show the correspondence between the oscillations in the spontaneous emission
rate and the closed-orbits of emitted photon going away and returning to the
atom inside the wedge. The closed-orbits can be readily constructed using the
method of images and they are also extracted from the spontaneous emission
rate.Comment: 8 figure
Observed Effects of a Changing Step-Edge Density on Thin-Film Growth Dynamics
We grew SrTiO3 on SrTiO3 [001] by pulsed laser deposition, while observing
x-ray diffraction at the (0 0 .5) position. The drop dI in the x-ray intensity
following a laser pulse contains information about plume-surface interactions.
Kinematic theory predicts dI/I = -4sigma(1-sigma), so that dI/I depends only on
the amount of deposited material sigma. In contrast, we observed experimentally
that |dI/I| < 4sigma(1-sigma), and that dI/I depends on the phase of x-ray
growth oscillations. The combined results suggest a fast smoothing mechanism
that depends on surface step-edge density.Comment: 4 figure
X-ray scattering from surfaces: discrete and continuous components of roughness
Incoherent surface scattering yields a statistical description of the
surface, due to the ensemble averaging over many independently sampled volumes.
Depending on the state of the surface and direction of the scattering vector
relative to the surface normal, the height distribution is discrete,
continuous, or a combination of the two. We present a treatment for the
influence of multimodal surface height distributions on Crystal Truncation Rod
scattering. The effects of a multimodal height distribution are especially
evident during in situ monitoring of layer-by-layer thin-film growth via Pulsed
Laser Deposition. We model the total height distribution as a convolution of
discrete and continuous components, resulting in a broadly applicable
parameterization of surface roughness which can be applied to other scattering
probes, such as electrons and neutrons. Convolution of such distributions could
potentially be applied to interface or chemical scattering. Here we find that
this analysis describes accurately our experimental studies of SrTiO3
annealing and homoepitaxial growth.Comment: 15 pages, 7 figure
Seeking children's perspectives: a respectful layered research approach
This article discusses why researchers and educators might choose to seek children's perspectives. It also highlights some of the key considerations when seeing children as having the right to contribute to decisions that affect them. The article draws on findings from a study that used pedagogically oriented methods for researching three- and four-year-old children's perspectives about outdoor spaces in the early childhood setting they attended. The article discusses the possibilities and practicalities of this research approach for both research and pedagogy. Examples are provided for others who may be considering working/researching in these ways
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