Laser Therapy in Correction of Optimization of Surgical Endointoxication

The problem of endointoxication remains one of the most urgent in modern surgery. The severity of the intoxication syndrome is determined not only by the intensity of the entry of toxic substances into the bloodstream from the lesion focus, but also by the adequacy of the functioning of the main mechanisms of detoxification - elimination of toxins. Clinical and laboratory studies of 62 patients with acute peritonitis were performed. To this end, daily sessions of laser therapy with “Matrix” were conducted for 5 days after the operation, using a head of KLO3 (radiation with a wavelength of 635 nm, 2 mW). Laser irradiation of blood through the skin in the projection of the ulnar vein was performed for 30 minutes. Using laser therapy for patients with acute peritonitis led to the reduction of the endogenous intoxication severity. The level of medium-mass molecules (λ = 280 nm) decreased by 13.3-26.2% compared to the control, the level of average-weight molecules (λ = 254 nm) decreased by 15.5-32.6% (p <0.05) against the background of laser therapy. Thus, the use of laser therapy in patients with acute peritonitis has led to a decrease in the severity of endogenous intoxication. One of the significant components of this treatment is its ability to improve microcirculation and, as a result, to correct lipid peroxidation and hypoxia, which reduces catabolic phenomena (one of the sources of endogenous intoxication). Clinical and laboratory studies have established that the effectiveness of such treatment decreases with severe forms of peritonitis

Некоторые аспекты управления репродукционной функцией у самок осетровых рыб

Two series of experiments on the effect of E-selenium on the reproductive function of sturgeons were conducted with different ways of introducing the drug into the body: through feed and injection. The effectiveness of the action of E-selenium was shown in two experiments. First, its ability to accelerate the process of maturation of oocytes was revealed due to the stimulation of generative metabolism and bringing it into line with the stages of the sexual cycle based on the biochemical parameters of the blood. An increase in the concentration in the blood by 1.4 times by the end of the 50-day experiment of total protein and beta-lipoproteins, which include ovovitelin (vitellogenin) during the period of vitellogenesis, as well as a moderate increase in haemoglobin concentration, indicates an increase in generative metabolism. It was noted that the metabolism of control fish was directed to somatic growth to a greater extent than that of experimental fish, as a result of which the average weight of females in control was 1.5 times higher than in the first series of experiments and weight of experimental fish was the same and increased by 6% in power in the second series. The role of the drug as a stabilizer of the physiological state of females, due to its high antioxidant properties, has been noted. When used, the existing signs of a stress state disappear, in contrast to the control fish in which they are detected: an increased cholesterol level, an erythrocyte sedimentation rate (ESR), and a decrease in haemoglobin concentration. The most significant effect from the use of the drug was obtained when the feed was irrigated with E-selenium, in which the number of mature fish after 50 days of the experiment was 2.7 times greater than in control, with a simultaneous halving of immature fish. In the case of the injection of females with this preparation, the number of fish at the IV stage of gonadal maturity in the experimental group after 50 days increased by 1.7 times. In the control group, on the contrary, it decreased by 1.2 times, with a constant proportion of females by the III stage of gonadal maturity.Проведены две серии экспериментов по влиянию препарата Е-селен на репродуктивную функцию осетровых рыб с разными способами внедрения препарата в организм: через корм и инъецированием. Показана эффективность действия Е-селена в двух сериях экспериментов. Выявлена его способность ускорять процесс созревания ооцитов вследствие стимуляции генеративного обмена и приведения его в соответствие с этапами полового цикла исходя из биохимических показателей крови. На усиление генеративного обмена указывает повышение концентрации в крови в 1,4 раза к концу 50-суточного эксперимента общего белка и бета-липопротеидов, в состав которых в период вителлогенеза входит ововителин (вителлогенин), а также умеренное увеличение концентрации гемоглобина. Отмечено, что обмен веществ у контрольных рыб в большей мере, чем у экспериментальных, направлен на соматический рост, вследствие чего средняя масса самок в контроле была выше, чем опыте, в первой серии экспериментов в 1,5 раза при неизменной массе опытных рыб и увеличении на 6% у контрольных во второй серии. Отмечена роль препарата как стабилизатора физиологического состояние самок из-за его высоких антиоксидантных свойств. При его применении исчезают имеющиеся признаки стрессового состояния, в отличие от контрольных рыб, у которых они выявляются: повышенный уровень холестерина, скорость оседания эритроцитов (СОЭ), снижение концентрации гемоглобина. Наибольший эффект от применения препарата получен при орошении Е-селеном корма, при этом количество созревших рыб после 50 суток эксперимента было в 2,7 раза больше, чем в контроле, с одновременным снижением в 2 раза числа незрелых рыб. В случае инъецирования самок этим препаратом количество рыб, находящихся на IV стадии зрелости гонад, у экспериментальной группы по истечении 50 суток увеличилось в 1,7 раза, у контрольных, наоборот, снизилось в 1,2 раза при неизменной доле самок на III стадии зрелости гонад