An intervention study of the effect of implementing Salmonella-controlled feeding strategies in Salmonella-high prevalence herds

In this study we found a significant effect of implementing a salmonella-controlling feeding strategy for finishers, consisting of a pelleted feed where non-heat treated wheat or barley was added to the feed after pelleting. But not all trial herds reached Salmonella level I within the observation period, according to the Danish Salmonella Surveillance System, indicating the multi-factorial nature of Salmonella-infections. One issue that needs further research is the effect of the Salmonella level before entering the finishing period

The effect of feeding pellets, meal and heat treatment on the Salmonella-prevalence of finishing pigs

A previous trial (I) has shown that meal reduces the Salmonella prevalence and the degree of stomach changes as compared with pelleted feed. It was also found that coarsely ground feed tended to reduce the Salmonella problem compared with finely ground feed. The type of feed that reduced the Salmonella prevalence resulted in a substantially poorer feed conversion. There is a need to clarify what the feed industry can do to produce feed that reduces the risk of Salmonella problems and gastric changes and at the same time to clarify the effect on feed conversion. Thus, the object of the study was to clarify the effect of the pelleting process and the expansion of grain on the proportion of serological reagents against Salmonella and on gastric health and production result in finishing pigs

Differential insulitic profiles determine the extent of beta cell destruction and the age at onset of type 1 diabetes

Published onlineJOURNAL ARTICLEType 1 diabetes (T1D) results from a T-cell mediated destruction of pancreatic beta cells following the infiltration of leukocytes (including CD8+, CD4+ and CD20+ cells) into and around pancreatic islets ("insulitis"). Recently, we reported that two distinct patterns of insulitis occur in patients with recent-onset T1D from the UK and that these differ principally in the proportion of infiltrating CD20+ B-cells (designated "CD20Hi" and "CD20Lo" respectively). We have now extended this analysis to include patients from the nPOD (USA) and DiViD (Norway) cohorts and confirm that the two profiles of insulitis occur more widely. Moreover, we show that patients can be directly stratified according to their insulitic profile and that those receiving a diagnosis before the age of 7 years always display the CD20Hi profile. By contrast, individuals diagnosed beyond the age of 13 years are uniformly defined as CD20Lo. This implies that the two forms of insulitis are differentially aggressive and that patients with a CD20Hi profile lose their beta cells at a more rapid rate. In support of this, we also find that the proportion of residual insulin-containing islets (ICIs) increases in parallel with age at onset of T1D. Importantly, those diagnosed in, or beyond, their teenage years retain ∼40% ICIs at diagnosis, implying that a functional deficit rather than absolute beta cell loss may be causal for disease onset in these patients. We conclude that appropriate patient stratification will be critical for correct interpretation of the outcomes of intervention therapies targeted to islet-infiltrating immune cells in T1D

Optimizing individual benefits of pulmonary rehabilitation including a multifaceted dietary intervention – A single-arm feasibility study

IntroductionFew studies have examined the effectiveness of nutrition interventions in municipal COPD rehabilitation programs. The objectives of this study were to examine the feasibility of implementation in practice (primary) and the feasibility of study methods and potential effectiveness (secondary) of a multidisciplinary dietary intervention offered to patients who start municipal rehabilitations program.MethodsThis study was a single arm intervention study with a pre-post design. Participant were recruited from five different municipal rehabilitation centers and received three individualized dietary counselling's. The primary outcome was retention, compliance to the intervention and complement of data collection. Secondary outcomes included changes in dietary intake, body composition and physical function. Analysis of the primary and secondary outcomes was primarily based on descriptive statistics.ResultsIn total, 111 (77%) of 145 eligible patients from five different municipalities consented to participate. Of them 99 (89%) completed. Before the intervention 67 (63%) of the participants had 75% or more of their requirement of energy covered, and 48 (45%) had 75% or more of their protein requirements covered. At the end of the intervention, 76 (77%) of the participants had 75% or more coverage of energy requirements and 80 (83%) had 75% or more coverage of their protein requirements. In general, the level of completeness was high.ConclusionWe found recruitment to be feasible, a high rate of retention, a high compliance to the intervention and high completeness of the data collection

Influence of dietary administration of organic acids and increased feed structure on S. typhimurium infection in pigs

Several epidemiological investigations and reports from advisors practicing in swine herds have independent of each other pointed out commercial feed mixes compared to home mixed feed and dry feed compared to wet fermented feed to be the most important risk factors for increased Salmonella infection levels in Danish slaughter pigs (Dahl (1997), Stege (1997))

Implementation of an Optimal First-Order Method for Strongly Convex Total Variation Regularization

We present a practical implementation of an optimal first-order method, due to Nesterov, for large-scale total variation regularization in tomographic reconstruction, image deblurring, etc. The algorithm applies to $\mu$-strongly convex objective functions with $L$-Lipschitz continuous gradient. In the framework of Nesterov both $\mu$ and $L$ are assumed known -- an assumption that is seldom satisfied in practice. We propose to incorporate mechanisms to estimate locally sufficient $\mu$ and $L$ during the iterations. The mechanisms also allow for the application to non-strongly convex functions. We discuss the iteration complexity of several first-order methods, including the proposed algorithm, and we use a 3D tomography problem to compare the performance of these methods. The results show that for ill-conditioned problems solved to high accuracy, the proposed method significantly outperforms state-of-the-art first-order methods, as also suggested by theoretical results.Comment: 23 pages, 4 figure

Parental rating of sleep in children with attention deficit/hyperactivity disorder

Objective: Sleep problems have often been associated with attention deficit/hyperactivity disorder (ADHD). Parents of those with ADHD and children with ADHD report sleep difficulties more frequently than healthy children and their parents. The primary objective of this paper is to describe sleep patterns and problems of 5 to 11-year-old children suffering from ADHD as described by parental reports and sleep questionnaires. Method: The study included 321 children aged 5–11 years (average age 8.4 years); 45 were diagnosed with ADHD, 64 had other psychiatric diagnoses, and 212 were healthy. One hundred and ninety-six of the test subjects were boys and 125 were girls. A semi-structured interview (Kiddie-SADS-PL) was used to DSM-IV diagnose ADHD and comorbidity in the clinical group. Sleep difficulties were rated using a structured sleep questionnaire (Children Sleep Behaviour Scale). Results: Children diagnosed with ADHD had a significantly increased occurrence of sleep problems. Difficulties relating to bedtime and unsettled sleep were significantly more frequent in the ADHD group than in the other groups. Children with ADHD showed prolonged sleep onset latency, but no difference was shown regarding numbers of awakenings per night and total sleep time per night. Comorbid oppositional defiant disorder appeared not to have an added effect on problematic behaviour around bedtime. Conclusion: Parents of children with ADHD report that their children do not sleep properly more often than other parents. The ADHD group report problems with bedtime resistance, problems with sleep onset latency, unsettled sleep and nightmares more often than the control groups. It may therefore be relevant for clinicians to initiate a closer examination of those cases reporting sleep difficulties