Significant differences in incubation times in sheep infected with bovine spongiform encephalopathy result from variation at codon 141 in the PRNP gene

The susceptibility of sheep to prion infection is linked to variation in the PRNP gene, which encodes the prion protein. Common polymorphisms occur at codons 136, 154 and 171. Sheep which are homozygous for the A<sub>136</sub>R<sub>154</sub>Q<sub>171</sub> allele are the most susceptible to bovine spongiform encephalopathy (BSE). The effect of other polymorphisms on BSE susceptibility is unknown. We orally infected ARQ/ARQ Cheviot sheep with equal amounts of BSE brain homogenate and a range of incubation periods was observed. When we segregated sheep according to the amino acid (L or F) encoded at codon 141 of the PRNP gene, the shortest incubation period was observed in LL141 sheep, whilst incubation periods in FF<sub>141</sub> and LF<sub>141</sub> sheep were significantly longer. No statistically significant differences existed in the expression of total prion protein or the disease-associated isoform in BSE-infected sheep within each genotype subgroup. This suggested that the amino acid encoded at codon 141 probably affects incubation times through direct effects on protein misfolding rates

Metabolic and cardiovascular adaptation, monkey. NASA SMD 3, project 76, experiment 44 conducted at NASA/JSC, 14-25 May 1977

The biomedical results from an experiment on a monkey subjected to space flight conditions are reported. A background history of the development and testing of an experiment system designed to permit measurement of physiological parameters in subhuman primates during continuous, comfortable, couch restraint for periods of up to 30 days is reviewed. Of major importance in the experimental design of the system was the use of a fiberglass pod, which could be sealed and subdivided into upper and lower parts, to monitor and control the physiological responses for various parts of the animal's body. The experiment was conducted within the Spacelab Simulator for a period of 11 days. Data recorded includes: Spacelab Simulator cabin temperature; ventilation rate; pod internal temperature; fraction percent oxygen; fraction percent carbon dioxide; oxygen consumption rate; carbon dioxide production rate; respiratory quotient; intrathoracic temperature; heart rate; mean aortic pressure; mean ventricular pressure; diurnal variation of parameters measured; comparison of mean preflight, flight, and postflight values of the parameters measured; and correlation matrix for the parameters measured

Experimentally exploring compressed sensing quantum tomography

In the light of the progress in quantum technologies, the task of verifying the correct functioning of processes and obtaining accurate tomographic information about quantum states becomes increasingly important. Compressed sensing, a machinery derived from the theory of signal processing, has emerged as a feasible tool to perform robust and significantly more resource-economical quantum state tomography for intermediate-sized quantum systems. In this work, we provide a comprehensive analysis of compressed sensing tomography in the regime in which tomographically complete data is available with reliable statistics from experimental observations of a multi-mode photonic architecture. Due to the fact that the data is known with high statistical significance, we are in a position to systematically explore the quality of reconstruction depending on the number of employed measurement settings, randomly selected from the complete set of data, and on different model assumptions. We present and test a complete prescription to perform efficient compressed sensing and are able to reliably use notions of model selection and cross-validation to account for experimental imperfections and finite counting statistics. Thus, we establish compressed sensing as an effective tool for quantum state tomography, specifically suited for photonic systems.Comment: 12 pages, 5 figure

Nitrogen Options to Increase Yields for Stockpiling Cool Season Grass in Eastern Ohio, USA

Stockpiling cool season grass for grazing in the fall and winter is an option to extend the grazing season. The purpose of this study was to determine the effects of yield and quality by adding urea, urea with Agrotain®, and ammonium sulfate to cool season grasses, primarily fescue and orchardgrass. This was the third year of the three location study in Southeast Ohio. Each location had a randomized complete block design with four treatments (control, 112 kg urea ha-1, 112 kg urea ha-1 plus Agrotain®, and 245.5 kg ammonium sulfate ha-1) and four replications of each treatment. The application date was August 6, 2018 and the plots were harvested on November 4, 2018. There were no statistical differences in crude protein, acid detergent fiber, and total digestible nutrients (CP, ADF, and TDN) (P \u3c 0.05). There were significant differences in yield between the control and all the treatments, but not within the treatments. The three-site average for the control was 2682 kg/ha-1; urea, 3431 kg/ha-1; urea+Agrotain®, 3855 kg/ha-1; and ammonium sulfate, 3468 kg/ha-1. Rainfall in the first 30 days from trial initiation in 2018 ranged from 8.15 to 9.47 cm, and the first significant rainfall (0.28-0.64 cm) was within 30 hours of initiation. This was the third and final year of this study and year three results continue to indicate that adding nitrogen increases yields

Environmental influences predominate in remission from alcohol use disorder in young adult twins

Background. Familial influences on remission from alcohol use disorder (AUD) have been studied using family history of AUD rather than family history of remission. The current study used a remission phenotype in a twin sample to examine the relative contributions of genetic and environmental influences to remission

Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice.

BACKGROUND: Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum that encodes a thermophilic beta-glucosidase. RESULTS: SYNbglA was generated by introducing codon substitutions to remove CpG motifs as these are associated with gene silencing in mammalian cells. SYNbglA expression can be localized in situ or detected quantitatively in colorimetric assays and can be co-localized with E. coli beta-galactosidase. Further, we have generated a Cre-reporter mouse in which SYNbglA is expressed following recombination to demonstrate the general utility of SYNbglA for in vivo analyses. SYNbglA can be detected in tissue wholemounts and in frozen and wax embedded sections. CONCLUSIONS: SYNbglA will have general applicability to developmental and molecular studies in vitro and in vivo.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

An Automated Method for the Detection and Extraction of HI Self-Absorption in High-Resolution 21cm Line Surveys

We describe algorithms that detect 21cm line HI self-absorption (HISA) in large data sets and extract it for analysis. Our search method identifies HISA as spatially and spectrally confined dark HI features that appear as negative residuals after removing larger-scale emission components with a modified CLEAN algorithm. Adjacent HISA volume-pixels (voxels) are grouped into features in (l,b,v) space, and the HI brightness of voxels outside the 3-D feature boundaries is smoothly interpolated to estimate the absorption amplitude and the unabsorbed HI emission brightness. The reliability and completeness of our HISA detection scheme have been tested extensively with model data. We detect most features over a wide range of sizes, linewidths, amplitudes, and background levels, with poor detection only where the absorption brightness temperature amplitude is weak, the absorption scale approaches that of the correlated noise, or the background level is too faint for HISA to be distinguished reliably from emission gaps. False detection rates are very low in all parts of the parameter space except at sizes and amplitudes approaching those of noise fluctuations. Absorption measurement biases introduced by the method are generally small and appear to arise from cases of incomplete HISA detection. This paper is the third in a series examining HISA at high angular resolution. A companion paper (Paper II) uses our HISA search and extraction method to investigate the cold atomic gas distribution in the Canadian Galactic Plane Survey.Comment: 39 pages, including 14 figure pages; to appear in June 10 ApJ, volume 626; figure quality significantly reduced for astro-ph; for full resolution, please see http://www.ras.ucalgary.ca/~gibson/hisa/cgps1_survey

A Neutral Hydrogen Self-Absorption Cloud in the SGPS

Using data from the Southern Galactic Plane Survey (SGPS) we analyze an HI self-absorption cloud centered on l = 318.0 deg, b = -0.5 deg, and velocity, v = -1.1 km/s. The cloud was observed with the Australia Telescope Compact Array (ATCA) and the Parkes Radio Telescope, and is at a near kinematic distance of less than 400 pc with derived dimensions of less than 5 x 11 pc. We apply two different methods to find the optical depth and spin temperature. In both methods we find upper limit spin temperatures ranging from 20 K to 25 K and lower limit optical depths ~ 1. We look into the nature of the HI emission and find that 60-70% originates behind the cloud. We analyze a second cloud at the same velocity centered on l = 319 deg and b = 0.4 deg with an upper limit spin temperature of 20 K and a lower limit optical depth of 1.6. The similarities in spin temperature, optical depth, velocity, and spatial location are evidence the clouds are associated, possibly as one large cloud consisting of smaller clumps of gas. We compare HI emission data with 12CO emission and find a physical association of the HI self-absorption cloud with molecular gas.Comment: 33 pages, 17 figures, 5 tables; Accepted for publication in ApJ. A version with higher quality images availabe at http://www.astro.umn.edu/~dkavars/ms.p