A comparative study of diagnostic and imaging techniques for osteoarthritis of the trapezium

Objectives. The aims of this study were to determine whether micro-CT is a reliable investigation method to evaluate the severity of OA in the trapezium and to develop a novel micro-CT scoring system based on a quantitative assessment of the subchondral bone thickness in order to better assess OA through an objective parameter.Methods. We compared different diagnostic and imaging techniques performed consecutively on each sample: X-ray, visual analysis, micro-CT and histology. OA and healthy trapezia were subjected to semi-quantitative and quantitative analyses to be classified in four degrees of severity in OA (control, OA-2, OA-3 and OA-4). Specifically, samples were analysed using Dell's score for X-ray, Brown's score for visual analysis and Mankin's score for histology. Micro-CT was scored using a novel quantitative scoring system based on subchondral bone thickness measurements. Results obtained with each technique were then compared and correlated.Results. X-ray analysis showed a higher frequency of OA-2 (27%) and OA-3 (32%) compared with OA-4 (5%), whereas visual analysis, micro-CT and histology showed a lower percentage for OA-2 (18%, 18% and 14%) and OA-3 (23%) and increased frequency for OA-4 (45%, 32% and 40%). Only the micro-CT score of subchondral bone thickness correlated significantly with all the other techniques (P < 0.05).Conclusion. This is the first comparison of techniques proposing a novel scoring system based on objective and quantitative micro-CT data that can be applied as a useful diagnostic tool for OA, providing a deeper comprehension of the pathophysiology of OA in trapezium

Modeling data entry and operations in WebML

Web Modeling Language (WebML, http://webml.org) is a notation for visually specifying complex Web sites at the conceptual level. All the concepts of WebML are specified both graphically and in XML; in particular, navigation and composition abstractions are based on a restricted number of hypertext components (units) which are assembled into pages and interconnected by links. During implementation, pages and units are automatically translated into server-side scripting templates, which enable the display of data dynamically retrieved from heterogeneous data sources. This paper extends WebML with data entry and operation units, for gathering information from clients and invoking arbitrary operations. Predefined operations are also proposed as built-in primitives for supporting standard updates on the content of the underlying data sources (represented as entities and relationships). This natural extension of WebML permits the visual modeling of Web pages integrating read and write acces..

Model-driven design of service-enabled web applications

Significant efforts are currently invested in application integration to enable the interaction and composition of business processes of different companies, yielding complex, multi-party processes. Web service standards, based on WSDL, have been adopted as a process-to-process communication paradigm. This paper presents an industrial experience in integrating data-intensive and process-intensive Web applications through Web services. Design of sites and of Web services interaction exploits modern Web engineering methods, including conceptual modeling, model verification, visual data marshalling and automatic code generation. In particular, the applied method is based on a declarative model for specifying data-intensive Web applications that enact complex interactions, driven by the user, with remote processes implemented as services. We describe the internal architecture of the CASE tool that has been used, and give an overview of three industrial applications developed with the described approach. 1

Snow depth time series retrieval by time-lapse photography: Finnish and Italian case studies

The capability of time-lapse photography to retrieve snow depth time series was tested. Historically, snow depth has been measured manually by rulers, with a temporal resolution of once per day, and it is a time-consuming activity. In the last few decades, ultrasonic and/or optical sensors have been developed to obtain automatic and regular measurements with higher temporal resolution and accuracy. The Finnish Meteorological Institute Image Processing Toolbox (FMIPROT) has been used to retrieve the snow depth time series from camera images of a snow stake on the ground by implementing an algorithm based on the brightness difference and contour detection. Three case studies have been illustrated to highlight potentialities and pitfalls of time-lapse photography in retrieving the snow depth time series: Sodankylä peatland, a boreal forested site in Finland, and Gressoney-La-Trinité Dejola and Careser Dam, two alpine sites in Italy. This study presents new possibilities and advantages in the retrieval of snow depth in general and snow depth time series specifically, which can be summarized as follows: (1) high temporal resolution - hourly or sub-hourly time series, depending on the camera's scan rate; (2) high accuracy levels - comparable to the most common method (manual measurements); (3) reliability and visual identification of errors or misclassifications; (4) low-cost solution; and (5) remote sensing technique - can be easily extended in remote and dangerous areas. The proper geometrical configuration between camera and stake, highlighting the main characteristics which each single component must have, has been proposed. Root mean square errors (RMSEs) and Nash-Sutcliffe efficiencies (NSEs) were calculated for all three case studies comparing with estimates from both the FMIPROT and visual inspection of images directly. The NSE values were 0.917, 0.963 and 0.916, while RMSEs were 0.039, 0.052 and 0.108 m for Sodankylä, Gressoney and Careser, respectively. In terms of accuracy, the Sodankylä case study gave better results. The worst performances occurred at Careser Dam located at 2600 m a.s.l., where extreme weather conditions and a low temporal resolution of the camera occur, strongly affecting the clarity of the images

Bacterial nanocellulose and its surface modification by glycidyl methacrylate and ethylene glycol dimethacrylate. Incorporation of vancomycin and ciprofloxacin

Among nanocelluloses, bacterial nanocellulose (BNC) has proven to be a promising candidate in a range of biomedical applications, from topical wound dressings to tissue-engineering scaffolds. Chemical modifications and incorporation of bioactive molecules have been obtained, further increasing the potential of BNC. This study describes the incorporation of vancomycin and ciprofloxacin in BNC and in modified BNC to afford bioactive BNCs suitable for topical wound dressings and tissue-engineering scaffolds. BNC was modified by grafting glycidylmethacrylate (GMA) and further cross-linking with ethylene glycol dimethacrylate (EGDMA) with the formation of stable C\u2013C bonds though a radical Fenton-type process that involves generation of cellulose carbon centred radicals scavenged by methacrylate structures. The average molar substitution degree MS (MS = methacrylate residue per glucose unit, measured by Fourier transform infrared (FT\u2013IR) analysis) can be modulated in a large range from 0.1 up to 3. BNC-GMA, BNC-EGDMA and BNC-GMA-EGDMA maintain the hydrogel status until MS reaches the value of 1. The mechanical stress resistance increase of BNC-GMA and BNC-GMA-EGDMA of MS around 0.8 with respect to BNC suggests that they can be preferred to BNC for tissue-engineering scaffolds in cases where the resistance plays a crucial role. BNC, BNC-GMA, BNC-EGDMA and BNC-GMA-EGDMA were loaded with vancomycin (VC) and ciprofloxacin (CP) and submitted to release experiments. BNC-GMA-EGDMA of high substitution degree (0.7\u20131) hold up to 50 percentage of the loaded vancomycin and ciprofloxacin amount, suggesting that they can be further investigated for long-term antimicrobial activity. Furthermore, they were not colonized by Staphylococcus aureus (S.A.) and Klebsiella pneumonia (K.P.). Grafting and cross-linking BNC modification emerges from our results as a good choice to improve the BNC potential in biomedical applications like topical wound dressings and tissue-engineering scaffolds