Investigating the Optimal Size of Anticancer Nanomedicine

Nanomedicines (NMs) offer new solutions for cancer diagnosis and therapy. However, extension of progression-free interval and overall survival time achieved by Food and Drug Administration-approved NMs remain modest. To develop next generation NMs to achieve superior anticancer activities, it is crucial to investigate and understand the correlation between the physicochemical properties of NMs (particle size in particular) and their interactions with biological systems to establish criteria for NM optimization. Here, we systematically evaluated the size-dependent biological profiles of three monodisperse drug–silica nanoconjugates (NCs; 20, 50, and 200 nm) through both experiments and mathematical modeling and aimed to identify the optimal size for the most effective anticancer drug delivery. Among the three NCs investigated, the 50-nm NC shows the highest tumor tissue retention integrated over time, which is the collective outcome of deep tumor tissue penetration and efficient cancer cell internalization as well as slow tumor clearance, and thus, the highest efficacy against both primary and metastatic tumors in vivo

Heterochromatin protein 1 is recruited to various types of DNA damage

Heterochromatin protein 1 (HP1) family members are chromatin-associated proteins involved in transcription, replication, and chromatin organization. We show that HP1 isoforms HP1-α, HP1-β, and HP1-γ are recruited to ultraviolet (UV)-induced DNA damage and double-strand breaks (DSBs) in human cells. This response to DNA damage requires the chromo shadow domain of HP1 and is independent of H3K9 trimethylation and proteins that detect UV damage and DSBs. Loss of HP1 results in high sensitivity to UV light and ionizing radiation in the nematode Caenorhabditis elegans, indicating that HP1 proteins are essential components of DNA damage response (DDR) systems. Analysis of single and double HP1 mutants in nematodes suggests that HP1 homologues have both unique and overlapping functions in the DDR. Our results show that HP1 proteins are important for DNA repair and may function to reorganize chromatin in response to damage

Preliminary Study on the Influence of Headphones for Listening Music on Hearing Loss of Young People

The paper presents results of hearing loss measurements provided for 81 young people (from 16 to 25 years old). The main aim of the work was to find the influence of headphones of the types used (closed, semi-open, open and in-ear) on the hearing losses. The first part of the research was to answer questions about the influence of: time of listening, loudness of music, other noise exposures as well as the type of the headphones used. It turned out that all factors mentioned above influence thresholds of hearing but the found dependencies are not explicit. The greatest hearing losses were observed for people who work as sound reinforcement engineers and, moreover, no influence of the headphone types was found for them. It turned out that the use of in-ear headphones causes the greatest hearing losses for some subjects (thresholds shifted up to about 20 dB HL at 4 kHz). The daily time of a listening also affected the hearing thresholds. It was found that for users of in-ear and close headphones, an average time of musical exposure of three hours causes the hearing loss of 10-15 dB HL at higher frequencies. The use of open as well as semi-open headphones has no influence on the hearing damage. Thus it would be stated that these kinds are safety in use. Almost 15% of the investigated young people have their thresholds shifted up at higher frequencies, particularly at 4 kHz, which means that they have the first symptoms of a permanent hearing damage

Preliminary Study on the Influence of Headphones for Listening Music on Hearing Loss of Young People

The paper presents results of hearing loss measurements provided for 81 young people (from 16 to 25 years old). The main aim of the work was to find the influence of headphones of the types used (closed, semi-open, open and in-ear) on the hearing losses. The first part of the research was to answer questions about the influence of: time of listening, loudness of music, other noise exposures as well as the type of the headphones used. It turned out that all factors mentioned above influence thresholds of hearing but the found dependencies are not explicit. The greatest hearing losses were observed for people who work as sound reinforcement engineers and, moreover, no influence of the headphone types was found for them. It turned out that the use of in-ear headphones causes the greatest hearing losses for some subjects (thresholds shifted up to about 20 dB HL at 4 kHz). The daily time of a listening also affected the hearing thresholds. It was found that for users of in-ear and close headphones, an average time of musical exposure of three hours causes the hearing loss of 10-15 dB HL at higher frequencies. The use of open as well as semi-open headphones has no influence on the hearing damage. Thus it would be stated that these kinds are safety in use. Almost 15% of the investigated young people have their thresholds shifted up at higher frequencies, particularly at 4 kHz, which means that they have the first symptoms of a permanent hearing damage

Sound-Absorbing and Insulating Enclosures for Ultrasonic Range

The physical phenomena occurring in sound-absorbing and insulating enclosures are subject of the present paper. These phenomena are: absorption in air and by the sound-absorbing material covering the walls and the coincidence effect. The absorption in the air can be neglected in small size enclosures for low ultrasonic frequencies (20–30 kHz). The coincidence plays a role in decrease of the sound insulation, however the main role play the leaks. The boards made of ceramic fibers have been chosen as the optimal sound-absorbing material. They are dense and have deeply porous structures. The enclosure for insulation of 20-kHz noise produced by a welding machine has been designed and manufactured, and reductions of 25 dB of peak and Leq levels have been achieved