A loop-mediated isothermal amplification (LAMP) assay for the detection of Cryptotermes brevis West Indian drywood termite (Blattodea: Kalotermitidae)

Cryptotermes brevis is one of the most destructive invasive termites in the subtropics and tropics and is a common biosecurity intercept at the Australian border. Drywood termite species are cryptic and difficult to identify morphologically in situations when soldiers or imagos are unavailable. We developed a novel DNA based loop-mediated isothermal amplification (LAMP) assay to detect C. brevis and differentiate it from other drywood termites. Validated voucher specimens of 30 different drywood termite species were obtained from several insect collections from which DNA was extracted and amplified. The amplicons containing partial mitochondrial 16S rRNA were sequenced and a DNA database was created from which C. brevis LAMP primers were developed, optimized, and tested. The assay was assessed against a range of target and non-target species and found to be specific, successfully amplifying the target specimens of C. brevis in under 30 min. Amplification success was variable against C. brevis faecal pellets due to minute, unmeasurable or degraded DNA. This LAMP test is a new tool for the rapid detection of C. brevis that will enable faster and less destructive management of drywood termite infestations

The Position of High Frequency Waves with Respect to the Granulation Pattern

High frequency velocity oscillations were observed in the spectral lines Fe I 543.45nm and 543.29nm, using 2D spectroscopy with a Fabry- Perot and speckle reconstruction, at the VTT in Tenerife. We investigate the radial component of waves with frequencies in the range 8 - 22mHz in the internetwork, network and a pore. We find that the occurrence of waves do not show any preference on location and are equally distributed over down-flows and up-flows, regardless of the activity of the observed area in the line of Fe I 543.45nm. The waves observed in the lower formed line of Fe I 543.29nm seem to appear preferentially over down-flows.Comment: Article has 12 pages and 7 images. It is accepted in Solar Physics Journa

Plants for planting ; indirect evidence for the movement of a serious forest pathogen, Teratosphaeria destructans, in Asia

Fungal diseases caused by native pathogens and pathogens introduced with planting stock have a significant impact on exotic plantation forestry in the tropics. Teratosphaeria destructans (formerly Kirramyces destructans) is a serious pathogen causing leaf, bud and shoot blight diseases of Eucalyptus spp. in plantations in the sub-tropics and tropics of south-east Asia. This pathogen was first discovered in Indonesia in 1995 and has subsequently spread to Thailand, China, Vietnam and East Timor. The biology, ecology and genetics of this important pathogen have not been explored yet. The objective of this study was, thus, to determine the genetic diversity and movement of T. destructans throughout south-east Asia using multi-gene phylogenies and microsatellite markers. Out of nine gene regions only two microsatellite markers detected a very low nucleotide polymorphism between isolates; seven other gene regions, ITS, β-tubulin, EF1-α, CHS, ATP6 and two microsatellite loci, reflected genetic uniformity. The two polymorphic molecular markers resolved six haplotypes among isolates from Indonesia and only a single haplotype elsewhere in Asia. The low diversity observed among isolates in the region of the first outbreak is as expected for a small founder population. The spread of a single clone over large distances throughout the region supports the hypothesis of spread via the human-mediated movement of germplasm.Murdoch University Doctoral Research Scholarship, University of Pretoria

Mutual relationship of oscillations in the frequency range 3.6 mHz to 22 mHz in the solar chromosphere

High frequency acoustic oscillations were suggested as the source of mechanical heating in the chromosphere. In this work the radial component of the oscillations in the frequency interval 3 mHz to 22 mHz are investigated. The observations were performed using "D spectrometry on the Fe I neutral spectral line at 543:45 nm. The high frequency oscillations of different frequencies appear to be associated with different spatial scales. It seems that different sources produce high and low frequency acoustic oscillations

International plant trade and biosecurity

This chapter explores the current status of plant trade and international biosecurity regulatory mechanisms to safeguard economic, social and economic well being of nations, states and economic regions. We provide an account of the international biosecurity framework in a historical context. In doing so we outline some of the common approaches to managing and regulating biosecurity risks associated with the plant horticultural export trade. This exploration identifies many of the inconsistencies in the application of plant biosecurity measured internationally. The approaches for regulation of live plants are compared amongst regions and future improvements are identified

Phomoxins B and C: polyketides from an endophytic fungus of the genus eupenicillium

Chemical investigations of the culture broth from an endophytic fungus Eupenicillium sp. have afforded two natural products phomoxins B (1) and C (2) as well as the previously reported fungal metabolite eupenoxide (3). Compounds 1 and 2 both contain a cyclic carbonate moiety that is rare among natural products. This paper reports the full spectroscopic characterisation of phomoxins B (1) and C (2) by NMR, UV, IR and MS data. All compounds were inactive against a panel of nosocomial microbes