Cypc19*17 Polymorphism as a Risk-factor for Nsaids-induced Ulcers

The new risk-factors for peptic ulcers induced by the use of nonsteroidal antiinflammatory drugs, such as polymorphism of different isoenzymes of cytochrome P450 were considered in the article. The aim of the research was to study different genetic polymorphism of several ferments CYP2C9 and CYP2C19 in inclination to NSAIDS-gastropathies by the way of estimation the risk of appearance of Helicobacter pylori (HP)-positive or Hp-negative NSAIDS- induced peptic ulcers, complicated or not with upper gastrointestinal bleeding.124 persons were examined (76 men, 48 women in the age of 56,2+/–9,1 years) with Hp-positive or Hp-negative NSAIDS-induced peptic ulcers, that were performed genotyping of isoferments of cytochrome system (CYP2C9, CYP2C19). Based on investigations of 5 different isoenzymes (CYP 2C9*2, CYP 2C9*3, CYP 2C19*2, CYP 2C19*3 and CYP 2C19*17). It was founded that peptic ulcers are strictly associated only with CYP 2C19*17-genotype, possibly due to its involvement in arachidonic acid metabolism and gastroprotection. Thus, polymorphism CYP 2C19*17 can be considered as one of the risk factors for NSAID-gastropathy though the future researches are needed

Photoneutrons for Radiation Therapy and Radionuclide Production

The possibility of organizing neutron therapy with a photoneutron beam produced by the electron accelerator target, and ensuring the required dose at the tumor at a reasonable exposure time and with minimal impact on patients investigated. Generation of neutrons from the target of electron accelerator takes place in two stages: e- ® γ ® n, and in the selected electron energy range of 20-100 MeV, the bremsstrahlung gamma radiation in many times (~ 3 orders of magnitude) offers more than “useful” neutron yield. This raises the problem of the selective control of the “harmful” for radiotherapy secondary gamma radiation while providing the minimum attenuation of the neutron flux in the output beam. In order to solve the general problem of the formation of a neutron beam with necessary spectral characteristics having sufficient intensity, there has been resolved a number of computational tasks of the selection of the optimal configuration of the output beam unit and its composition. The matter of high importance is to minimize additional irradiation of the patient from the bremsstrahlung (generated by electrons) and secondary gamma radiation (generated by neutrons) from the accelerator target as well as from output unit’s materials. On the other hand, at a generation stage e- ® γ the bremsstrahlung beam could be applied for effective radionuclide production by reactions (γ,n) and (γ,p) due to high leak intensity ~ 1.3·1017 photon/s. By the Mo100(γ,n)99Mo reaction the main diagnostic nuclide 99Tc could be produced sufficiently for the clinical needs. The resulting configuration of the output unit provides the required beam quality for the neutron capture therapy (NCT), which commonly assumed to be the only competitive technology of neutron therapy on the background of the massive invasion of proton therapy and other highly selective techniques that ultimately damage the target sparing the surrounding tissues and organs. For the accessible accelerator (average current 4 mA and electron energy 35 MeV) the flux density of epithermal photoneutrons (they required for NCT) in the beam at the output is of the order of magnitude or more higher than the typical yield from existing and planned reactors' beams. The proposed scheme of generation and extraction of photoneutrons for NCT has a number of strong advantages over traditional techniques: a) the applying of electron accelerators for neutron production is much safer and cheaper than conventional reactor beams in use; b) accelerator with the target, the beam output unit with the necessary equipment can be placed on the territory of the clinic without any problems of radiation safety; c) the proposed target – liquid gallium, which also serves as a cooler, is an “environmentally friendly” material due to low activation which rapidly (in ~ 4 days) falls to the background level

Visualization of Murine Intranasal Dosing Efficiency Using Luminescent Francisella tularensis: Effect of Instillation Volume and Form of Anesthesia

Intranasal instillation is a widely used procedure for pneumonic delivery of drugs, vaccine candidates, or infectious agents into the respiratory tract of research mice. However, there is a paucity of published literature describing the efficiency of this delivery technique. In this report we have used the murine model of tularemia, with Francisella tularensis live vaccine strain (FTLVS) infection, to evaluate the efficiency of pneumonic delivery via intranasal dosing performed either with differing instillation volumes or different types of anesthesia. FTLVS was rendered luminescent via transformation with a reporter plasmid that constitutively expressed the Photorhabdus luminescens lux operon from a Francisella promoter. We then used an IVIS Spectrum whole animal imaging system to visualize FT dissemination at various time points following intranasal instillation. We found that instillation of FT in a dose volume of 10 µl routinely resulted in infection of the upper airways but failed to initiate infection of the pulmonary compartment. Efficient delivery of FT into the lungs via intranasal instillation required a dose volume of 50 µl or more. These studies also demonstrated that intranasal instillation was significantly more efficient for pneumonic delivery of FTLVS in mice that had been anesthetized with inhaled (isoflurane) vs. parenteral (ketamine/xylazine) anesthesia. The collective results underscore the need for researchers to consider both the dose volume and the anesthesia type when either performing pneumonic delivery via intranasal instillation, or when comparing studies that employed this technique

Химический состав аэрозоля в приземном слое прибрежной зоны Восточной Антарктиды

Chemical composition of aerosol in the ground layer of the coastal zone in East Antarctica is analyzed in the article. The aerosol samples were taken in 2006–2015 during seasonal works of the Russian Antarctic Expeditions (RAE), namely, these were 52nd–53rd, 55th, and 58th–60th expeditions. Samples were taken in the 200‑km band of the sea-shore zone along routes of the research vessels (REV) «Akademik Fedorov» and «Akademik Treshnikov» as well as on territories of the Russian stations Molodezhnaya and Mirny. Although the results obtained did show the wide range of the aerosol concentrations and a certain variability of their chemical composition, some common features of the variability were revealed. Thus, during the period from 2006 to 2014 a decrease of average values of the sums were noted. Spatially, a tendency of decreasing of the ion concentrations was found in the direction from the station Novolazarevskaya to the Molodezhnaya one, but the concentrations increased from the Molodezhnaya to the station Mirny. The sum of ions of the aerosol in the above mentioned coastal zone was, on the average, equal to 2.44 μg/m3, and it was larger than that on the territory of the Antarctic stations Molodezhnaya (0,29 μg/m3) and Mirny (0,50 ág / m3). The main part to the sum of the aerosol ions on the Antarctic stations was contributed by Na+, Ca2+, Cl−, SO4 2−. The main ions in aerosol composition in the coastal zone are ions Na+ and Cl−. The dominant contribution of the sea salt and SO4 2− can be traced in not only the composition of atmospheric aerosols, but also in the chemical composition of the fresh snow in the coastal areas of East Antarctica: at the Indian station Maitri, on the Larsemann Hills, and in a boring located in 55.3 km from the station Progress (K = 1.4÷6.1). It was noted that values of the coefficient of enrichment K of these ions decreases as someone moves from a shore to inland. Estimation of contributions of the continental and maritime factors to formation of the aerosol chemical composition revealed higher enrichment ratios for K+, Ca2+, SO4 2− (K = 3.6÷13.0). This reflects not only influence of the natural sources, but the intensity of human activities on the Antarctic continent as well. The elemental composition of solid aerosols was also analyzed. The largest concentrations were determined for Zn, Al and Fe. The ratio of concentration of the elements in both the soluble and insoluble phases of the aerosol showed that 84.1% of the total amount of the elements was contained in a water-insoluble state. Fractional relation between the element concentrations changed in different phases from 16 to 98%. High enrichment of the aerosol particles by Zn, Cu, Cr, Ba, Pb, Ni,Se, As, Cd (the enrichment factors = 27÷26 445) had been revealed. The content of dominant chemical components (Na+, Cl−, Zn, Fe), factors and coefficients of the element enrichment in the aerosols as well as in fresh snow of the coastal zone of East Antarctica are indicative of the identity of sources where their composition is formed.Рассмотрена временнáя и пространственная изменчивость химического состава атмосферного аэрозоля, отобранного в период сезонных работ 52, 53, 55, 58–60‑й РАЭ по маршруту следования НЭС «Академик Федоров» и «Академик Трешников» от станции Новолазаревская до станции Мирный. Заметное снижение суммы ионов в аэрозоле в 2007–2008 гг. хорошо согласуется с исследованиями в районе Восточной Атлантики и связано с гидрологическими процессами в Атлантическом океане. Установлено высокое обогащение аэрозольных частиц Zn, Cu, Cr, Ba, Pb, Se, As, Ni, Cd. Содержание преобладающих химических компонентов (Na+, Cl−, Zn, Fe), факторы и коэффициенты обогащения элементов (ФО = 27÷26 445) и ионов (K = 3,6÷13,0) в аэрозоле в поверхностном и свежевыпавшем снеге (K = 1,4÷6,1) прибрежной части Восточной Антарктиды свидетельствуют об идентичности источников формирования их состава

Химический состав приземного атмосферного аэрозоля в Баренцбурге (архипелаг Шпицберген) по результатам многолетних исследований

The chemical composition (ions, elements, polycyclic aromatic hydrocarbons) of aerosol and gaseous impurities (SO2, HNO3, HCl, NH3) in the surface layer of the atmosphere in Barentsburg, located on the Western Svalbard island (Svalbard archipelago), is analyzed. Atmospheric aerosol and gaseous impurities brought to the Arctic from middle latitudes and deposited on snow and ice not only interact with various natural objects, but also spread to long distances with melting dirty snow and ice. Air sampling was carried out following to methodology adopted by the international networks of the atmospheric monitoring programs in South-East Asia (EANET) and Europe (EMEP). In 2011-2015, the observations of the chemical composition of the atmospheric ground layer were performed daily during the light season (April–September), and monthly from April 2016 to 2018. The largest total ion concentrations were observed in 2011–2012. Seasonal variability of ion concentrations in the aerosol was characterized by high values in the cold period (October–February) and low values in the warm one (May–June). High values of the coefficient of correlation between ions Na+ and Cl− (r = 0,93) as well as between Mg2+ and Cl−  (r = 0,81) throughout the year show that the main source of the aerosol is the sea surface. The significant correlation between ions K+, NO3-, NH4+, SO42−, K+, SO42− in the polar night point to the influence of local sources: coal mining at the mine and its3 combustion at thermal power plants. Emission of polycyclic aromatic hydrocarbons and the gaseous impurities (SO2, HNO3) into the atmosphere, especially during the polar night, is also influenced by local sources. Among the elements the maximum enrichment of the aerosol was revealed for As, Cr, Zn, Mo, Cd, Sn, Sb, W, and Pb with a low content of Cd, Sn, Sb, W, and Pb in the coal, sludge and on the underlying surface. On the basis of the elemental composition of the aerosol and the back-trajectory analysis, it was shown that the air masses enriched in heavy metals come to the area of the Barentsburg settlement from middle latitudes.Прослежена межгодовая (2011–2017 гг.) и сезонная (2016–2018 гг.) изменчивость компонентов химического состава (ионы, элементы, полициклические ароматические углеводороды) атмосферного аэрозоля и газообразных примесей (SO2, HNO3, HCl, NH3) в приземной атмосфере западной части Российской Арктики (пос. Баренцбург на Шпицбергене)

To the nature of 0+ states in 228Th studied by two-neutron transfer

Sequences of states observed in the 230Th(p, t)228Th reaction are selected which can be treated as rotational bands and as multiplets of excitations. Moments of inertia have been derived from these sequences, whose values may be considered as evidence of the two-phonon nature of most 0+ excitations. Experimental data are compared with interacting boson model and quasiparticle-phonon model calculations and with experimental data for 229Pa. Conclusions have been made concerning the nature of 0+ states in 228Th

Nuclear g factors and structure of high-spin isomers in 190,192,194 Pt and 196,198 Hg

The integral perturbed angular distribution (IPAD) method in an external magnetic field has been used to measure the g factors of isomers in the 190,192,194Pt and 196,198Hg nuclei, populated in the (α, 2n)-reaction. The results are as follows: 190Pt, g(