35 research outputs found
ΠΠΠΠ’ΠΠ¦ΠΠΠΠΠΠ ΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠ Π‘Π’ΠΠ₯ΠΠ‘Π’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠΠ§Π Π£ΠΠ ΠΠΠΠΠΠΠ― ΠΠΠΠΠ‘ΠΠΠ
The article is devoted to popular and important approach to optimization of stock management systems, which is based on costs minimization with account of uncertainties. The simulation and optimization mathematical model of decision-making for management of a trading company is described in the article.Π‘ΡΠ°ΡΡΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½Π° Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΠΌΡ ΡΠ΅Π³ΠΎΠ΄Π½Ρ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΊ ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΡΠΈΡΡΠ΅ΠΌ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π·Π°ΠΏΠ°ΡΠ°ΠΌΠΈ, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΠΎΠΌΡ Π½Π° ΠΌΠΈΠ½ΠΈΠΌΠΈΠ·Π°ΡΠΈΠΈ ΠΈΠ·Π΄Π΅ΡΠΆΠ΅ΠΊ Ρ ΡΡΠ΅ΡΠΎΠΌ Π½Π΅ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΡΡΠΈ. Π ΡΡΠ°ΡΡΠ΅ ΠΎΠΏΠΈΡΠ°Π½Π° ΠΈΠΌΠΈΡΠ°- ΡΠΈΠΎΠ½Π½ΠΎ-ΠΎΠΏΡΠΈΠΌΠΈΠ·Π°ΡΠΈΠΎΠ½Π½Π°Ρ ΠΌΠ°ΡΠ΅ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ ΠΏΡΠΈΠ½ΡΡΠΈΡ ΡΠ΅ΡΠ΅Π½ΠΈΠΉ ΠΏΠΎ Π²ΠΎΠΏΡΠΎΡΠ°ΠΌ ΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡΡ ΡΠΎΡΠ³ΠΎΠ²ΠΎΠΉ ΠΊΠΎΠΌΠΏΠ°Π½ΠΈΠΈ
Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae
BACKGROUND: Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRFl and eRF3. eRFl recognizes nonsense codons UAA, UAG and UGA, while eRF3 stimulates polypeptide release from the ribosome in a GTP- and eRFl β dependent manner. Recent studies has shown that proteins interacting with these release factors can modulate the efficiency of nonsense codon readthrough. RESULTS: We have isolated a nonessential yeast gene, which causes suppression of nonsense mutations, being in a multicopy state. This gene encodes a protein designated Itt1p, possessing a zinc finger domain characteristic of the TRIAD proteins of higher eukaryotes. Overexpression of Itt1p decreases the efficiency of translation termination, resulting in the readthrough of all three types of nonsense codons. Itt1p interacts in vitro with both eRFl and eRF3. Overexpression of eRFl, but not of eRF3, abolishes the nonsense suppressor effect of overexpressed Itt1p. CONCLUSIONS: The data obtained demonstrate that Itt1p can modulate the efficiency of translation termination in yeast. This protein possesses a zinc finger domain characteristic of the TRIAD proteins of higher eukaryotes, and this is a first observation of such protein being involved in translation
Natural Phaeosphaeride A Derivatives Overcome Drug Resistance of Tumor Cells and Modulate Signaling Pathways
n the present study, natural phaeosphaeride A (PPA) derivatives are synthesized. Anti-tumor studies are carried out on the PC3, K562, HCT-116, THP-1, MCF-7, A549, NCI-H929, Jurkat, and RPMI8226 tumor cell lines, and on the human embryonic kidney (HEK293) cell line. All the compounds synthesized turned out to have better efficacy than PPA towards the tumor cell lines listed. Among them, three compounds exhibited an ability to overcome the drug resistance of tumor cells associated with the overexpression of the P-glycoprotein by modulating the work of this transporter. Luminex xMAP technology was used to assess the effect of five synthesized compounds on the activation of intracellular kinase cascades in A431 cells. MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex was used, which allowed for the simultaneous detection of the following nine phosphorylated protein markers of the main intracellular signaling pathways: a universal transcription factor that controls the expression of immune-response genes, apoptosis and cell cycle NFΞΊB (pS536); cAMP-dependent transcription factor (CREB (pS133); mitogen-activated kinase p38 (pT180/pY182); stress-activated protein kinase JNK (pT183/pY185); ribosomal SK; transcription factors STAT3 (pS727) and STAT5A/B (pY694/699); protein kinase B (Akt) (pS473); and kinase regulated by extracellular signals ERK1/2 (pT185/pY187). The effect of various concentrations of PPA derivatives on the cell culture was studied using xCelligence RTCA equipment. The compounds were found to modulate JNK, ERK1/2, and p38 signaling pathways. The set of activated kinase cascades suggests that oxidative stress is the main probable mechanism of the toxic action of PPA derivatives
Er,Yb:ReGa3(BO3)4 (Re = Y, Gd) laser crystals
Phase relationships in the ErxYbyY1-x-yGa3(BO3)4-Bi2O3-B2O3-(Y,Er,Yb)2O3βGa2O3 and ErxYbyGd1-x-yGa3(BO3)4-Bi2O3-B2O3-(Gd,Er,Yb)2O3 β Ga2O3 (x = 0.02, y = 0.11 at.%) system were studied in the temperature range from 1000 to 900 ΠΎΠ‘. Multicomponent melt Bi2O3-Ga2O3-B2O3-(Y,Gd)2O3 were used as reasonable fluxes for high-temperature solution growth of ErxYbyR1-x-yGa3(BO3)4 (R = Y, Gd) spontaneous crystals. The segregation coefficients of Yb and Er impurities in the obtained crystals are determined. The unit cell parameters for the grown crystals were studied, also showing the micromorphology characteristics of the crystals. The luminescence kinetics were investigated, and the lifetimes of the 4I13/2 energy level of Er3+ ions for Er,Yb:ReGa3(BO3)4 crystals were determined
ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² ΡΠΈΡΠΎΡΠΊΠ΅Π»Π΅ΡΠ° β ΡΠΈΡΠΎΠΊΠ΅ΡΠ°ΡΠΈΠ½ΠΎΠ² ΠΈ Π±Π΅ΡΠ°-III ΡΡΠ±ΡΠ»ΠΈΠ½Π° Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ ΠΊΡΠ»ΡΡΡΡ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΈΠ· ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΠΠΠΠ¦ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈΠΌ. Π. Π. ΠΠ»ΠΎΡ ΠΈΠ½Π°
Introduction. Despite advances in the treatment of melanoma, the results of therapy cannot be considered satisfactory, and the search for new drugs and effective combinations of medicine continues. The drugs are being developed aimed at reducing the metastatic tumor potential β migrastatics. The targets of the drugs can be cytoskeletal proteins of tumor cells β cytokeratin (CK) intermediate filaments and microtubule protein beta-III tubulin (TUBB3).Aim. To estimate of the CK and TUBB3 expression in melanoma cell lines to form an informative in vitro cell model for screening and studying migrastatics.Materials and methods. The molecular phenotype of 21 human melanoma cell lines from the collection of N. N. Blokhin National Medical Research Center of Oncology, and 18 of which were isolated from tumor metastases in the lymph nodes, soft tissues or subcutaneously. The level of TUBB3 expression and de novo expression of CKs in vimentin-expressing cells (CK + Vim) were assessed by an immunofluorescent method and flow cytometry.Results. Beta-III tubulin expression was detected in all cultures studied, de novo expression of CKs was found in 20 / 21 lines. The exception was primary uveal melanoma 92-1, that did not express CK + Vim. Both parameters significantly differed between the cells of the studied panel: CK + Vim co-expression β from 0 to 91 %, TUBB3 β from 18 to 86 %. No correlation was found between the expression level of TUBB3 and CK + Vim (Pearsonβs correlation coefficient r = 0.11; p = 0.65). Three groups of the cell lines with different ratio of TUBB3 expression and CK + Vim co-expression were identified: 1) similar level of expression of both markers; 2) the level of co-expression of CK + Vim more or less high than the index for TUBB3; 3) the level of TUBB3 expression more or less high than the index for CK + Vim co-expression.Conclusion. A panel of 21 human melanoma cell lines was formed with quantitatively estimated expression of cytoske-letal proteins responsible for the migration activity of tumor cells β CKs and TUBB3. Groups of the lines with different expression ratio of the markers can be used for screening and preclinical evaluation potential migrastatics that reduce the metastatic potential of melanoma and may reduce resistance to taxanes.ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅. ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° Π΄ΠΎΡΡΠΈΠΆΠ΅Π½ΠΈΡ Π² Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ, ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π½Π΅Π»ΡΠ·Ρ ΠΏΡΠΈΠ·Π½Π°ΡΡ ΡΠ΄ΠΎΠ²Π»Π΅ΡΠ²ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌΠΈ, ΠΈ ΠΏΠΎΠΈΡΠΊ Π½ΠΎΠ²ΡΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΠΊΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΠΉ Π»Π΅ΠΊΠ°ΡΡΡΠ² ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ°Π΅ΡΡΡ. Π Π°Π·ΡΠ°Π±Π°ΡΡΠ²Π°ΡΡΡΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ, Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ Π½Π° ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ, β ΠΌΠΈΠ³ΡΠ°ΡΡΠ°ΡΠΈΠΊΠΈ. Π’ΠΎΡΠΊΠΎΠΉ ΠΏΡΠΈΠ»ΠΎΠΆΠ΅Π½ΠΈΡ ΡΡΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΌΠΎΠ³ΡΡ ΡΠ²Π»ΡΡΡΡΡ Π±Π΅Π»ΠΊΠΈ ΡΠΈΡΠΎΡΠΊΠ΅Π»Π΅ΡΠ° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΠΊΠ»Π΅ΡΠΊΠΈ, ΠΊ ΠΊΠΎΡΠΎΡΡΠΌ ΠΎΡΠ½ΠΎΡΡΡΡΡ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΎΡΠ½ΡΠ΅ ΡΠΈΠ»Π°ΠΌΠ΅Π½ΡΡ β ΡΠΈΡΠΎΠΊΠ΅ΡΠ°ΡΠΈΠ½Ρ (Π¦ΠΊ) ΠΈ Π±Π΅Π»ΠΎΠΊ ΠΌΠΈΠΊΡΠΎΡΡΡΠ±ΠΎΡΠ΅ΠΊ Π±Π΅ΡΠ°-III ΡΡΠ±ΡΠ»ΠΈΠ½ (TUBB3).Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ°Π½Π΅Π»ΠΈ ΠΊΡΠ»ΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Ρ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ Π¦ΠΊ ΠΈ TUBB3 Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ in vitro Π΄Π»Ρ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΌΠΈΠ³ΡΠ°ΡΡΠ°ΡΠΈΠΊΠΎΠ².ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΉ ΡΠ΅Π½ΠΎΡΠΈΠΏ 21 ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΠΈΠ· ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π»ΠΈΠ½ΠΈΠΉ Π€ΠΠΠ£ Β«ΠΠ°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΡΠΊΠΈΠΉ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΡΠΊΠΈΠΉ ΡΠ΅Π½ΡΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈΠΌ. Π. Π. ΠΠ»ΠΎΡ
ΠΈΠ½Π°Β» ΠΠΈΠ½Π·Π΄ΡΠ°Π²Π° Π ΠΎΡΡΠΈΠΈ, ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
18 ΠΊΡΠ»ΡΡΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΠΈΠ· ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΎΠ² ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² Π»ΠΈΠΌΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ·Π»Ρ, ΠΌΡΠ³ΠΊΠΈΠ΅ ΡΠΊΠ°Π½ΠΈ ΠΈΠ»ΠΈ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎ. ΠΡΠ΅Π½ΠΊΠ° ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ TUBB3 ΠΈ de novo ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π¦ΠΊ Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
, ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠΈΡ
Π²ΠΈΠΌΠ΅Π½ΡΠΈΠ½ (ΠΠΈΠΌ) (Π¦ΠΊ + ΠΠΈΠΌ), ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΈΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ Ρ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΠ΅ΠΉ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ TUBB3 Π²ΡΡΠ²Π»Π΅Π½Π° Π²ΠΎ Π²ΡΠ΅Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
ΠΊΡΠ»ΡΡΡΡΠ°Ρ
, de novo ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π¦ΠΊ β Π² 20 ΠΈΠ· 21 ΠΊΡΠ»ΡΡΡΡΡ. ΠΡΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° ΠΏΠ΅ΡΠ²ΠΈΡΠ½Π°Ρ ΡΠ²Π΅Π°Π»ΡΠ½Π°Ρ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠ° 92-1, Π½Π΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠ°Ρ Π¦ΠΊ + ΠΠΈΠΌ. ΠΠ±Π° ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ°Π·Π»ΠΈΡΠ°Π»ΠΈΡΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΏΠ°Π½Π΅Π»ΠΈ: ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π¦ΠΊ + ΠΠΈΠΌ β ΠΎΡ 0 Π΄ΠΎ 91 %, TUBB3 β ΠΎΡ 18 Π΄ΠΎ 86 %. ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΎΠ²Π½ΡΠΌΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ TUBB3 ΠΈ Π¦ΠΊ + ΠΠΈΠΌ Π½Π΅ Π²ΡΡΠ²Π»Π΅Π½ΠΎ (ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ ΠΏΠΈΡΡΠΎΠ½Π° r = 0,11; p = 0,65). ΠΡΠ΄Π΅Π»Π΅Π½Ρ 3 Π³ΡΡΠΏΠΏΡ ΠΊΡΠ»ΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΡΠ°Π·Π½ΡΠΌ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠΎΠ²Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ TUBB3 ΠΈ ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π¦ΠΊ + ΠΠΈΠΌ: 1) ΡΡ
ΠΎΠ΄Π½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ²; 2) ΡΡΠΎΠ²Π΅Π½Ρ ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π¦ΠΊ + ΠΠΈΠΌ Π² Π±ΠΎΠ»ΡΡΠ΅ΠΉ ΠΈΠ»ΠΈ ΠΌΠ΅Π½ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΏΡΠ΅Π²ΡΡΠ°Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Π΄Π»Ρ TUBB3; 3) ΡΡΠΎΠ²Π΅Π½Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ TUBB3 Π² ΡΠ°Π·Π½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΏΡΠ΅Π²ΡΡΠ°Π΅Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΠΊΠΎΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π¦ΠΊ + ΠΠΈΠΌ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π‘ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π° ΠΏΠ°Π½Π΅Π»Ρ ΠΈΠ· 21 ΠΊΡΠ»ΡΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, Π² ΠΊΠΎΡΠΎΡΡΡ
ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π¦ΠΊ ΠΈ TUBB3 β Π±Π΅Π»ΠΊΠΎΠ² ΡΠΈΡΠΎΡΠΊΠ΅Π»Π΅ΡΠ°, ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΡΡ
Π·Π° ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡΡΠΏΠΏΡ ΠΊΡΠ»ΡΡΡΡ Ρ ΡΠ°Π·Π½ΡΠΌ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΡΠΈΡ
ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ ΡΠΊΡΠΈΠ½ΠΈΠ½Π³Π° ΠΈ Π΄ΠΎΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΡ
ΠΌΠΈΠ³ΡΠ°ΡΡΠ°ΡΠΈΠΊΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠΌΠ΅Π½ΡΡΠ°ΡΡ ΠΌΠ΅ΡΠ°ΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ ΠΈ ΠΌΠΎΠ³ΡΡ ΡΠ½ΠΈΠΆΠ°ΡΡ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΡ ΠΊ ΡΠ°ΠΊΡΠ°Π½Π°ΠΌ
SIMULATION MODELING IN THE STOCHASTIC PROBLEM OF STOCK MANAGEMENT
The article is devoted to popular and important approach to optimization of stock management systems, which is based on costs minimization with account of uncertainties. The simulation and optimization mathematical model of decision-making for management of a trading company is described in the article
A73
Human epidermal growth factor receptor-2 (HER2/nue) is overexpressed in breast cancer. It has been shown that HER2-targeted therapies have radically changed the outcome of HER2-positive breast cancer patients. The aim of this study was to develop the technique for preparation of recombinant anti-HER2/neu antibody from plants and to provide with the basic characteristics of the HER2/neu oncoprotein.
Materials and Methods: We have designed and synthesized viral vectors to transform Agrobacterium tumifaciens affinity, ion exchange and gel filtration chromatography were used to purify the antibody. The quality of the substance was confirmed by SDSβPAGE and ELISA. The biological activity was tested by immunofluorescent analysis.
Results: Antibodies were purified by affinity and ion exchange chromatography from the Agrobacterium tumifaciens leaves extracts. Gel filtration chromatography was used for final purification of the protein. Immunocytochemical staining was performed to test the functional activity of the plant-made antibodies. Here we also show that plant-made antibodies bind to HER2/neu receptors on the surface of human SK-BR-3 breast cancer cells as effectively as the diagnostic antibody A0485 (DAKO, Denmark). Flow cytometry analysis was used for quantitative estimation of recombinant anti-HER2/neu antibodies: from 75.7% to 98.3% cells bound the plant-made antibodies. The same data were obtained with trastuzumab.
Conclusion: Based on the data obtained, we conclude that plant-made antibodies inhibit HER2/neu+ breast cancer cell proliferation. Additional experiments are required to prove that trastuzumab and plant-made antibodies share full identity in their biological activity