The time-dependent effect of conflict on delays in the transposition of EU directives

This article analyses the effect of conflict in the Council of the European Union (EU) on delays in the transposition of EU directives. Based on enforcement and management theories, we predict that conflict in the Council speeds up the transposition process. In addition, we control for the instigation of infringement procedures by the Commission and expect a weaker effect of conflict in cases where the Commission disagrees with a directive and if directives grant more discretion to member states. These hypotheses are tested using two indicators of conflict: heterogeneity and polarization. Cox regression analysis is applied with timedependent effects and with a shared frailty to control for the multilevel structure of the data. The analyses show that, over time, conflict has an increasing negative effect on delays

Fluorescence-lifetime-limited trapping of Rydberg helium atoms on a chip

Metastable (1s)(2s) $^3{\rm S}_1$ helium atoms produced in a supersonic beam were excited to Rydberg-Stark states (with $n$ in the $27-30$ range) in a cryogenic environment and subsequently decelerated by, and trapped above, a surface-electrode decelerator. In the trapping experiments, the Rydberg atoms were brought to rest in 75~$\mu$s and over a distance of 33~mm and kept stationary for times $t_{\mathrm{trap}}$ in the $0-525$~$\mu$s range, before being re-accelerated for detection by pulsed field ionization. The use of a home-built valve producing short gas pulses with a duration of about 20~$\mu$s enabled the reduction of losses arising from collisions with atoms in the trailing part of the gas pulses. Cooling the decelerator to 4.7~K further suppressed losses by transitions induced by blackbody radiation and by collisions with atoms desorbing from the decelerator surface. The main contribution (60\%) to the atom loss during deceleration is attributed to the escape out of the decelerator moving traps of atoms having energies higher than the trap saddle point, spontaneous emission and collisions with atoms in the trailing part of the gas pulses causing each only about 20\% of the atom loss. At 4.7 K, the atom losses in the trapping phase of the experiments were found to be almost exclusively caused by spontaneous emission and the trap lifetimes were found to correspond to the natural lifetimes of the Rydberg-Stark states. Increasing the temperature to 100 K enhanced the trap losses by transitions stimulated by blackbody radiation

Elevated Plasma Levels of Vascular Endothelial Growth Factor and Hepatocyte Growth Factor: Clinical Significance and Correlation with Tumor Burden in Patients with Chronic Myeloid Leukemia

Angiogenesis is an established indispensable process in the development and metastasis of solid tumors. Its significance in the pathogenesis and progression of hematological malignancies is still to be elucidated. Data have recently been accumulated about its role in chornic myeloid leukemia (CML). The aims of our study were: (I) to assess vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) plasma levels in patients with newly diagnosed, untreated and treated CML; (II) to analyze the differences of their levels in varying phases of the disease; (III) to analyze VEGF and HGF correlation with some markers reflecting the tumor burden as well as the progression of the disease. Plasma levels of VEGF and HGF were determined by ELISA in 16 patients with CML and 21 healthy individuals. VEGF and HGF levels were followed-up in 6 patients according to the progression of the disease or the treatment regimen. All the patients with CML showed significantly higher levels of VEGF and HGF when compared to the control group. We found a significant correlation between HGF and leukocytes, LDH, splenomegaly and blast percentage in the peripheral blood. Significant correlation was found between VEGF and platelets, LDH and leukocytes. Our data indicate that CML are highly associated with elevated plasma levels of VEGF and HGF, wich corroborates the thesis of its angiogenic dependency. Likewise, the present study suggests that measurements of VEGF and HGF may be useful for assessing disease activity and progression as well

Provisional Arrest and Incarceration in the International Criminal Tribunals

This article examines the widely ignored but important issue regarding the provisional arrest and detention of persons suspected of having committed international crimes by international or internationalized courts. The paper examines the pioneer case law and practice of the United Nations International Criminal Tribunals for the former Yugoslavia and Rwanda, the Extraordinary Chambers in the Courts of Cambodia, and the Special Tribunal for Lebanon, as well as the emerging practice of the permanent International Criminal Court, to evaluate how these courts have generally addressed the rights of these individuals to due process and freedom from arbitrary arrest and detention before prosecutors seek formal charges against them. The authors argue that while the early international jurisprudence established apparently strong legal standards to preserve the rights of suspects, using doctrines such as abuse of process, these courts have generally failed to offer the meaningful remedies required to resolve manifest violations of such fundamental human rights by the detaining authorities. The article offers preliminary recommendations on how, going forward, the rights guaranteed to suspects allegedly involved in the worst crimes known to law in international(ized) courts may be better protected

How do supranational regulators keep companies in line?: An analysis of the enforcement styles of EU agencies

National governments have increasingly transferred enforcement powers to EU agencies that monitor and penalize non-compliance by private actors. How do EU agencies apply enforcement competences in practice? Based on the Eurolegalism thesis, pressures for deeper integration have led to the emergence of a more adversarial enforcement style in Europe. Consequently, supranational regulators are expected to employ formal and coercive enforcement instruments. Conversely, studies of EU enforcement suggest that EU agencies may be reluctant to antagonize national governments by prosecuting private actors. In this study, we analyze the enforcement practices of supranational agencies with direct enforcement powers: the EU Aviation Safety Agency (EASA), European Securities and Markets Authority (ESMA) and European Medicines Agency (EMA). We find that EU agencies apply a legalistic approach, but they vary in coerciveness of enforcement. Whereas EU agencies tend to apply more coercive measures against non-conform products, they are generally not adversarial toward non-compliant organizations.The politics and administration of institutional chang

REVIEW OF THE CURRENT STATUS AND FUTURE PERSPECTIVES ON PSEUDOGYMNOASCUS DESTRUCTANS STUDIES WITH REFERENCE TO SPECIES FINDINGS IN BULGARIA

Emerging infectious diseases are a well-known threat to the wildlife and require complex research. There is a rapidly accumulating knowledge on the infectious disease of bats, named firstly White Nose Syndrome (WNS) and afterwards – White Nose Disease (WND), and its causative agent – the pathogenic fungus Pseudogymnoascus destructans. Although mass mortality of bats, known since a decade, is currently restricted to North America, the pathogen is of global concern as a potential threat to other hibernating bat populations. Therefore five years after the first comprehensive synthesis on the fungal ecology and relevant knowledge gaps (FOLEY ET AL. 2011), we decided to summarize the published information on the pathogen morphology, reproduction, ecological requirements, geographic distribution and systematic position. In addition, the present review compiles the available data on the affected bat species, mechanisms of WND, on the host response and on the effective treatment strategies with possible methods for fighting the pathogen to reduce the mortality in affected regions as well. Special attention is paid to the finding of the fungus in Bulgarian caves

Improving osteoblasts cells proliferation via femtosecond laser surface modification of 3D-printed poly-ε-caprolactone scaffolds for bone tissue engineering applications

Synthetic polymer biomaterials incorporating cells are a promising technique for treatment of orthopedic injuries. To enhance the integration of biomaterials into the human body, additional functionalization of the scaffold surface should be carried out that would assist one in mimicking the natural cellular environment. In this study, we examined poly-epsilon-caprolactone (PCL) fiber matrices in view of optimizing the porous properties of the constructs. Altering the porosity of a PCL scaffold is expected to improve the material's biocompatibility, thus influencing its osteoconductivity and osteointegration. We produced 3D poly-epsilon-caprolactone (PCL) matrices by a fused deposition modeling method for bone and cartilage tissue engineering and performed femtosecond (fs) laser modification experiments to improve the surface properties of the PCL construct. Femtosecond laser processing is one of the useful tools for creating a vast diversity of surface patterns with reproducibility and precision. The processed surface of the PCL matrix was examined to follow the effect of the laser parameters, namely the laser pulse energy and repetition rate and the number (N) of applied pulses. The modified zones were characterized by scanning electron microscopy (SEM), confocal microscopy, X-ray computed tomography and contact angle measurements. The results obtained demonstrated changes in the morphology of the processed surface. A decrease in the water contact angle was also seen after fs laser processing of fiber meshes. Our work demonstrated that a precise control of material surface properties could be achieved by applying a different number of laser pulses at various laser fluence values. We concluded that the structural features of the matrix remain unaffected and can be successfully modified through laser postmodification. The cells tests indicated that the micro-modifications created induced MG63 and MC3T3 osteoblast cellular orientation. The analysis of the MG63 and MC3T3 osteoblast attachment suggested regulation of cells volume migration

PHARMACOGENETIC STUDY OF THE ACETYLATION PHENOTYPE IN A BULGARIAN POPULATION

N-acetyltransferase, an enzyme involved in the metabolic inactivation of drugs like isoniazide, some sulfonamides and others is well-known to he under polymorphic genetic control. The acetylation phenotype of the patients may serve as an important guide in foretelling the therapeutic efficacy or tolerahility of a particular drug. In the present study we investigated the distribution of the acetylaiion phenotypes in a group of 100 healthy volunteers of both sexes using sulfadimidine as a substrate. The distribution was found to follow a bimodal pattern, as aspected, with a slight predominance of the "slow" acetylators - in 58 % of the cases, a finding similar to literature data from neighbouring and other European countries. In the men's group the distribution was approximately the same as that in the whole group whilst in the women's one the "rapid" inactivators prevailed. This work represents the first modest attempt in Bulgaria for phenotyping the population according to the individual acetylaiion status