Synthesis And Radiolabeling Of Temozolomide Loaded Solid Lipid Nanoparticles

WOS: 00045501940201

99mTc labeled plumbagin: estrogen receptor dependent examination against breast cancer cells and comparison with PLGA encapsulated form

Plant origin products having anticancer properties come into prominence due to widespread of cancer. Plumbagin has various biological activities like anticancer activity. Estrogen receptor (ER) specificity of plumbagin (PL) and radiolabeled PL investigated by in vitro studies on ER+ and ER- adenocarcinoma cells. Additionally, PLGA encapsulation was carried out to reduce toxicity of plumbagin and encapsulation effect was investigated. Plumbagin radiolabeled with 100 % in yields and had ER specificity. Furthermore, PLGA encapsulation effected positively on properties of plumbagin; reduced toxicity, increased stability and ER specificity. A promising agent for the diagnosis of ER+ breast cancer is suggested. © 2015, Akadémiai Kiadó, Budapest, Hungary

Synthesis And Radiolabeling Of Temozolomide Loaded Solid Lipid Nanoparticles

WOS: 00045501940201

In vivo biological evaluation of I-131 radiolabeled-paclitaxel glucuronide (I-131-PAC-G)

WOS: 000305114100009Paclitaxel (PAC) is a natural occurring diterpene alkoloid originally isolated from the bark of Taxus Brevifolia. It is one of the most important antitumor agents for clinical treatment of ovarian, breast non-small cell lung and prostate cancers. It is known that these types of cancer cells have high beta-glucuronides enzyme which can catalyze the hydrolysis of glucuronides. This is why the synthesis compounds which undergo glucuronidation come into question in the imaging and therapy of these cancer cells. The aim of current study is conjugation of glucuronic acid (G) to the starting substance PAC, labeling with I-131 and to perform its in vivo biological evaluation. Glucuronic acid derived paclitaxel compound [paclitaxel-glucuronide (PAC-G)] was labeled with I-131 using iodogen method. According to thin layer radio chromatography (TLRC) method, the radio-chemical yield of I-131-PAC-G was 84.30 +/- 7.40% (n = 10). The biodistribution of I-131-PAC-G in healthy female and male Wistar Albino rats has been investigated. Imaging studies on male Balb-C mice were performed by using the Kodak FX PRO in vivo Imaging System. The range of the breast/blood, breast/muscle; ovary/blood, ovary/muscle ratios is approximately between 1.29 and 11.34 in 240 min, and between 0.71 and 8.24 in 240 min for female rats. The prostate/blood and prostate/muscle ratio is between 1.94 and 6.95 in 30 min for male rats. All these experimental studies indicate that I-131-PAC-G may potentially be used in breast, ovary and prostate tissues as an imaging agent. Also it is thought that (131)-PAC-G bears a theraphy potential because of the I-131 radionuclide and can be improved with further investigations

In vitro evaluation of radiolabeled (125I) methanol extracts of yarrow in cell lines of MCF-7, PC-3, A-549 and Caco-2

Nowadays, cancer is still the second leading cause of death all over the world. Therefore, natural products which have anticancer and antitumor properties are come into prominence. Achillea family is known with anticancer and antitumor activity. Yarrow which has over a hundred bioactive compounds is a member of Achillea family. In current study; components of yarrow which was obtained after methanol extraction and purification were radiolabeled with 125I and effects of these radiolabeled components on the cells were examined with using Caco-2, MCF-7, A-549, PC-3 cell lines. As a result of these studies, seven peaks were obtained and the highest radiolabeling yield was calculated for 125I radiolabeled Peak 7 (95.00 ± 7.07, n = 4). To screen the biological properties of these radiolabeled peaks at determined cell lines, our ongoing effort was to evaluate incorporation percentage with time dependent. Furthermore, 125I-Peak 7 had highest incorporation ratio for whole cell lines and its incorporation percentage was increased with time dependent. Results of these in vitro studies were compatible with previous in vivo studies and traditional use of yarrow plants. © 2012 Akadémiai Kiadó, Budapest, Hungary