Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland

Introduction. Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis. Objective. The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland. Materials and method. DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively. Results and conclusions. Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland

Experimental and theoretical study of the thermal solubility of the vacancy in germanium

Quenching experiments are performed using state of the art p-type Czochralski-grown Ge samples. The vacancy formation energy and thermal equilibrium concentration are determined on the basis of resistivity changes measured after quenching, using van der Pauw and Hall measurements. The results are compared with previously published experimental data and with results from analytical and ab initio calculations of the formation energy of the vacancy in different charge states. The deep levels introduced during the quenching step are studied with deep level transient spectroscopy and with FTIR, revealing besides the typical quenching related defects which are probably connected with vacancies also the presence of Cu

The evolution of natural alimentation

Advances in medicine and the evolution of health-related behaviors through time and across cultures have contributed to changes in attitudes toward natural alimentation. In the past, women who breastfed their babies were educated by word of mouth and family traditions passed on by their female relatives and communities, and the act of breastfeeding itself constituted a socio-cultural process. The health-related benefits of breast milk for infant nutrition have long been known. Throughout the ages, the image of the breastfeeding woman has inspired many artists and promoted natural alimentation. Ancient beliefs and religious practices were combined with the teachings of the church as well as alchemy principles, and lactation counseling was based on popular belief rather than scientific or medical knowledge. In modern times, breastfeeding has experienced a rise in popularity and is recommended to mothers during pregnancy and as part of contraception education. Anecdotal beliefs regarding the beneficial effects of breastfeeding on children’s overall psychological wellbeing have become a subject of scientific investigation. Within the current pregnancy-related standards, the modern promotion of breastfeeding encompasses nutritional, immunological and psychological aspects. In this paper we have summarized the evolution of the beliefs that have surrounded breastfeeding from antiquity to the present day

Mutations in mammalian tolloid-like 1 gene detected in adult patients with ASD

Atrial septal defect (ASD) is an incomplete septation of atria in human heart causing circulatory problems. Its frequency is estimated at one per 10 000. Actions of numerous genes have been linked to heart development. However, no single gene defect causing ASD has yet been identified. Incomplete heart septation similar to ASD was reported in transgenic mice with both inactive alleles of gene encoding mammalian zinc metalloprotease a mammalian tolloid-like 1 (tll1). Here, we have screened 19 ASD patients and 15 healthy age-matched individuals for mutations in TLL1 gene. All 22 exons were analyzed exon by exon for heteroduplex formation. Subsequently, DNA fragments forming heteroduplexes were sequenced. In four nonrelated patients, three missense mutations in coding sequence, and one single base change in the 5′UTR have been detected. Two mutations (Met182Leu, and Ala238Val) were detected in ASD patients with the same clinical phenotype. As the second mutation locates immediately upstream of the catalytic zinc-binding signature, it might change the enzyme substrate specificity. The third change, Leu627Val in the CUB3 domain, has been found in an ASD patient with interatrial septum aneurysm in addition to ASD. The CUB3 domain is important for substrate-specific recognition. In the remaining 15 patients as well as in 15 reference samples numerous base substitutions, deletions, and insertions have been detected, but no mutations changing the coding sequence have been found. Lack of mutations in relation to ASD of these patients could possibly be because of genetic heterogeneity of the syndrome