Influence of some Pesticides on Entomopathogenic Fungus Lecanicillium (=Verticillium) lecanii (Zimm.) ZARE & GAMS

An in vitro study was conducted to determine the interaction effect of ten pesticides tested at field recommended dose on conidial germination, vegetative growth and sporulation of Lecanicillium lecanii(ZIMM.) ZARE&GAMS. Compatibility of L. lecanii to different pesticides was found to be varied. Conidial germination was 99.3 and 85.7% in Pongamia oil and acephate, whereas, it was totally inhibited by the presence of chlorothalonil, iprodion + carbendazim, carbendazim and thiophanate methyl indicating that these pesticides were highly toxic. Dinocap recorded as moderately toxic while endosulfan, abamectin and ethion were least toxic based to the germination of conidia. So also Iprodion + carbendazim did not and carbendazim allow L. lecanii to put forth mycelium growth in their presence. Thiophanate methyl, Pongamia oil, acephate, endosulfan, ethion and chlorothalonil were observed to be innocuous pesticides registering growth of mycelium upto 2.33, 2.23, 2.23, 2.03, 2.03 and 2.00 cm dia., respectively, from 0.6 cm dia. held in the center of Petri plate on 14th day after treatment. As far as sporulation is concerned, Pongamia oil alone recorded the maximum yield of 47.2x106 conidia/ml followed by 18x106 conidia/ml, in chlorothalonil as against 20x106 conidia/ml in control, which means that the pongamia oil exhibited synergistic effect on L. lecanii, yielding more conidial spores. Thus, based on in vitro interaction study, pongamia oil alone was found to be safe to the entamopathogenic fungus L. lecanii in nature and iprodion + carbendazim and carbendazim were found to be highly toxic

Record of Thrips on Mango

During a trial in 2009 at Moorapoor Village, Dharmapuri district, Tamil Nadu, for control of mango hoppers and thrips using entomo-pathogens, inflorescences were seen to harbour different species of thrips. Close microscopic observation revealed presence of Frankliniella schultzei (Tryb.), Thrips palmi Karny, T. hawaiiensis (Morgan) and T. subnudula. However, Thrips palmi was the predominant species whereas, for the first time Frankliniella schultzai and Thrips subnudula (Karny, 1927) are reported here on inflorescence of mango in India

Direct Nitrate Reductase Assay versus Microscopic Observation Drug Susceptibility Test for Rapid Detection of MDR-TB in Uganda

The most common method for detection of drug resistant (DR) TB in resource-limited settings (RLSs) is indirect susceptibility testing on Lowenstein-Jensen medium (LJ) which is very time consuming with results available only after 2–3 months. Effective therapy of DR TB is therefore markedly delayed and patients can transmit resistant strains. Rapid and accurate tests suitable for RLSs in the diagnosis of DR TB are thus highly needed. In this study we compared two direct techniques - Nitrate Reductase Assay (NRA) and Microscopic Observation Drug Susceptibility (MODS) for rapid detection of MDR-TB in a high burden RLS. The sensitivity, specificity, and proportion of interpretable results were studied. Smear positive sputum was collected from 245 consecutive re-treatment TB patients attending a TB clinic in Kampala, Uganda. Samples were processed at the national reference laboratory and tested for susceptibility to rifampicin and isoniazid with direct NRA, direct MODS and the indirect LJ proportion method as reference. A total of 229 specimens were confirmed as M. tuberculosis, of these interpretable results were obtained in 217 (95%) with either the NRA or MODS. Sensitivity, specificity and kappa agreement for MDR-TB diagnosis was 97%, 98% and 0.93 with the NRA; and 87%, 95% and 0.78 with the MODS, respectively. The median time to results was 10, 7 and 64 days with NRA, MODS and the reference technique, respectively. The cost of laboratory supplies per sample was low, around 5 USD, for the rapid tests. The direct NRA and MODS offered rapid detection of resistance almost eight weeks earlier than with the reference method. In the study settings, the direct NRA was highly sensitive and specific. We consider it to have a strong potential for timely detection of MDR-TB in RLS

Review on Unmanned Aerial Vehicle Assisted Sensor Node Localization in Wireless Networks: Soft Computing Approaches

Node positioning or localization is a critical requisite for numerous position-based applications of wireless sensor network (WSN). Localization using the unmanned aerial vehicle (UAV) ispreferred over localization using fixed terrestrial anchor node (FTAN) because of low implementation complexity and high accuracy. The conventional multilateration technique estimates the position of theunknown node (UN) based on the distance from the anchor node (AN) to UN that is obtained from the received signal strength (RSS) measurement. However, distortions in the propagation medium may yield incorrect distance measurement and as a result, the accuracy of RSS-multilateration is limited. Though theoptimization based localization schemes are considered to be a better alternative, the performance of these schemes is not satisfactory if the distortions are non-linear. In such situations, the neural network (NN) architecture such as extreme learning machine (ELM) can be a better choice as it is a highly non-linearclassifier. The ELM is even superior over its counterpart NN classifiers like multilayer perceptron (MLP) and radial basis function (RBF) due to its fast and strong learning ability. Thus, this paper provides a comparative review of various soft computing based localization techniques using both FTAN and aerial ANs for better acceptability

Evidence-based national vaccine policy

India has over a century old tradition of development and production of vaccines. The Government rightly adopted self-sufficiency in vaccine production and self-reliance in vaccine technology as its policy objectives in 1986. However, in the absence of a full-fledged vaccine policy, there have been concerns related to demand and supply, manufacture vs. import, role of public and private sectors, choice of vaccines, new and combination vaccines, universal vs. selective vaccination, routine immunization vs. special drives, cost-benefit aspects, regulatory issues, logistics etc. The need for a comprehensive and evidence based vaccine policy that enables informed decisions on all these aspects from the public health point of view brought together doctors, scientists, policy analysts, lawyers and civil society representatives to formulate this policy paper for the consideration of the Government. This paper evolved out of the first ever ICMR-NISTADS national brainstorming workshop on vaccine policy held during 4-5 June, 2009 in New Delhi, and subsequent discussions over email for several weeks, before being adopted unanimously in the present form

Technical bulletin for NEH region on tuber crops genetic resources etc

Tropical Tuber crops such as cassava, sweet potato, yams, edible aroids, elephant foot yam, taro and tannia are the major sources of dietary energy, feeding billions of people across the globe. The north eastern region of India covering seven states such as Assam, Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland and Tripura are one of the major hotspots of biodiversity known for enormous genetic diversity of tuber crops. Considerable genetic diversity exists among the tropical tuber crops for plant type, morphological and physiological characteristics, reactions to diseases and pests, adaptability and distribution. The tropical tuber crops play a vital part in the diet of the tribal people. They are being cultivated mainly for food, poultry and piggery feed and for therapeutic purposes, although in a primitive way. The industrial application of tuber crops grown here is not well known. Imparting knowledge on the various aspects of tropical tuber crops cultivation such as modern agro-techniques, propagation techniques, high yielding varieties, nutritional value and value addition assumes significance. Hence, this technical bulletin entitled ‘Genetic resources, agro-techniques, value addition and utilisation in tropical tuber crops: current prospects for North Eastern states in India’ was prepared to provide required information for the benefit of tuber crop growers, processing Industries and consumers.Considerable genetic diversity exists among the tropical tuber crops for plant type, morphological and physiological characteristics, reactions to diseases and pests, adaptability and distribution. The tropical tuber crops play a vital part in the diet of the tribal people. They are being cultivated mainly for food, poultry and piggery feed and for therapeutic purposes, although in a primitive way. The industrial application of tuber crops grown here is not well known. Imparting knowledge on the various aspects of tropical tuber crops cultivation such as modern agro-techniques, propagation techniques, high yielding varieties, nutritional value and value addition assumes significance.ICAR- NEH Scheme CTCR