San Vicente

Esta obra es el resultado de una investigación llevada a cabo por profesores, adscriptos a la docencia, egresados y alumnos de la Facultad de Arquitectura, Urbanismo y Diseño de la Universidad Nacional de Córdoba, dirigidos por quien redacta este texto, Eduardo Barseghian. La tarea dió comienzo en 2005, sobre la base de búsquedas anteriores y no ha terminado todavía. Tanto esas búsquedas como las que están en curso han contado y cuentan con subsidios otorgados por la Secretaría de Ciencia y Técnica de la Universidad mencionada. Durante 2005, el eje dominante fue el espacio público del barrio General Paz, seguido por el barrio San Vicente en el período 2006-2007 y Alberdi en 2008 y 2009, Güemes a lo largo de 2010 y 2011 y San Martín entre los años 2012 y 2013. Las áreas pericentrales de Córdoba no son sino el conjunto de los actuales barrios Güemes, Alberdi (con Santa Ana), San Martín (incluidos Providencia, Ducasse e Independencia), Alta Córdoba (más Cofico), Pueyrredón (más Patria), General Paz (más Juniors y los llamados Alto y Bajo general Paz), San Vicente y Nueva Córdoba (al que cabe adicionarle el Parque Sarmiento, en razón de que ambos son contemporáneos y formaron parte de un mismo proyecto). Algunos de esos conjuntos residenciales fueron planificados como pueblos, otros surgieron de manera espontánea, por transformación paulatina del uso de sus suelos y en ciertos casos, estimulados por la presencia de obras singulares, significativas y de gran magnitud. Su designación como áreas, en el título, tiene la intención de abarcar, sin entrar en pormenores, todas las categorías territoriales que se les ha asignado hasta su constitución

Güemes

Libro sobre desarrollo urbano sustentable en Barrio Güemes.Esta obra es el resultado de una investigación llevada a cabo por profesores, adscriptos a la docencia, egresados y alumnos de la Facultad de Arquitectura, Urbanismo y Diseño de la Universidad Nacional de Córdoba, dirigidos por quien redacta este texto, Eduardo Barseghian. La tarea dió comienzo en 2005, sobre la base de búsquedas anteriores y no ha terminado todavía. Tanto esas búsquedas como las que están en curso han contado y cuentan con subsidios otorgados por la Secretaría de Ciencia y Técnica de la Universidad mencionada. Durante 2005, el eje dominante fue el espacio público del barrio General Paz, seguido por el barrio San Vicente en el período 2006-2007 y Alberdi en 2008 y 2009, Güemes a lo largo de 2010 y 2011 y San Martín entre los años 2012 y 2013. Las áreas pericentrales de Córdoba no son sino el conjunto de los actuales barrios Güemes, Alberdi (con Santa Ana), San Martín (incluidos Providencia, Ducasse e Independencia), Alta Córdoba (más Cofico), Pueyrredón (más Patria), General Paz (más Juniors y los llamados Alto y Bajo general Paz), San Vicente y Nueva Córdoba (al que cabe adicionarle el Parque Sarmiento, en razón de que ambos son contemporáneos y formaron parte de un mismo proyecto). Algunos de esos conjuntos residenciales fueron planificados como pueblos, otros surgieron de manera espontánea, por transformación paulatina del uso de sus suelos y en ciertos casos, estimulados por la presencia de obras singulares, significativas y de gran magnitud. Su designación como áreas, en el título, tiene la intención de abarcar, sin entrar en pormenores, todas las categorías territoriales que se les ha asignado hasta su constitución e integración definitivas como barrios

A fine physical map of the CACNA1A gene region on 19p13.1-p13.2 chromosome

The P/Q-type Ca(2+) channel alpha(1A) subunit gene (CACNA1A) was cloned on the short arm of chromosome 19 between the markers D19S221 and D19S179 and found to be responsible for Episodic Ataxia type 2, Familial Hemiplegic Migraine and Spinocerebellar Ataxia type 6. This region was physically mapped by 11 cosmid contigs spanning about 1. 4Mb, corresponding to less than 70% of the whole region. The cosmid contig used to characterize the CACNA1A gene accounted only for the coding region of the gene lacking, therefore, the promoter and possible regulation regions. The present study improves the physical map around and within the CACNA1A by giving a complete cosmid or BAC contig coverage of the D19S221-D19S179 interval. A number of new STSs, whether polymorphic or not, were characterized and physically mapped within this region. Four ESTs were also assigned to cosmids belonging to specific contigs

Forefront users\u2019 experience evaluation by employing together virtual reality and electroencephalography: A case study on cognitive effects of scents

Scents have the ability to affect peoples\u2019 mental states and task performance with to different extents. It has been widely demonstrated that the lemon scent, included in most all-purpose cleaners, elicits stimulation and activation, while the lavender scent elicits relaxation and sedative effects. The present study aimed at investigating and fostering a novel approach to evaluate users\u2019 experience with respect to scents\u2019 effects through the joint employment of Virtual Reality and users\u2019 neurophysiological monitoring, in particular Electroencephalography. In particular, this study, involving 42 participants, aimed to compare the effects of lemon and lavender scents on the deployment of cognitive resources during a daily life experience consisting in a train journey carried out in virtual reality. Our findings showed a significant higher request of cognitive resources during the processing of an informative message for subjects exposed to the lavender scent with respect to the lemon exposure. No differences were found between lemon and lavender conditions on the self\u2010reported items of pleasantness and involvement; as this study demonstrated, the employment of the lavender scent preserves the quality of the customer experience to the same extent as the more widely used lemon scent

Complete Loss of P/Q Calcium Channel Activity Caused by a CACNA1A Missense Mutation Carried by Patients with Episodic Ataxia Type 2

Familial hemiplegic migraine, episodic ataxia type 2 (EA2), and spinocerebellar ataxia type 6 are allelic disorders of the CACNA1A gene (coding for the α1A subunit of P/Q calcium channels), usually associated with different types of mutations (missense, protein truncating, and expansion, respectively). However, the finding of expansion and missense mutations in patients with EA2 has blurred this genotype-phenotype correlation. We report the first functional analysis of a new missense mutation, associated with an EA2 phenotype—that is, T→C transition of nt 4747 in exon 28, predicted to change a highly conserved phenylalanine residue to a serine at codon 1491, located in the putative transmembrane segment S6 of domain III. Patch-clamp recording in HEK 293 cells, coexpressing the mutagenized human α1A-2 subunit, together with human β4 and α2δ subunits, showed that channel activity was completely abolished, although the mutated protein is expressed in the cell. These results indicate that a complete loss of P/Q channel function is the mechanism underlying EA2, whether due to truncating or to missense mutations

Nitrate: its role on ruminal fermentation of beef cattle under intensified grazing system.

Rumen fermentation it is one of the contributors to the greenhouse gases emission (GHG) that causes global warming, and due to that reason livestock production is scrutinized and questioned all around the world on how GHG emissions from this subsector can be mitigated. A range of techniques have been studied by animal scientist, at nutritional level, to mitigate methane emission. Nitrate is of them, and it has a double important role on rumen fermentation, acting as a source of non-protein nitrogen, and as an electron sink path that allows the redirection of hydrogen to nitrate reduction, instead of being used in the methanogenesis process, and thus reducing methane emission. The partial results here presented shows that nitrate does not affect forage, supplement, and total dry mater intake, and neither short chain fatty acids production, however, methane emission (g.kg.day-1) and CO2eq emissions per kg. head-1d-1were reduced when nitrate was added in the diet. Thus, nitrate has the ability in changing rumen fermentation, which besides acting as source of non-protein nitrogen, also mitigate anthropic GHG emissions from enteric fermentation

The Histone Demethylase Jarid1b (Kdm5b) Is a Novel Component of the Rb Pathway and Associates with E2f-Target Genes in MEFs during Senescence

Senescence is a robust cell cycle arrest controlled by the p53 and Rb pathways that acts as an important barrier to tumorigenesis. Senescence is associated with profound alterations in gene expression, including stable suppression of E2f-target genes by heterochromatin formation. Some of these changes in chromatin composition are orchestrated by Rb. In complex with E2f, Rb recruits chromatin modifying enzymes to E2f target genes, leading to their transcriptional repression. To identify novel chromatin remodeling enzymes that specifically function in the Rb pathway, we used a functional genetic screening model for bypass of senescence in murine cells. We identified the H3K4-demethylase Jarid1b as novel component of the Rb pathway in this screening model. We find that depletion of Jarid1b phenocopies knockdown of Rb1 and that Jarid1b associates with E2f-target genes during cellular senescence. These results suggest a role for Jarid1b in Rb-mediated repression of cell cycle genes during senescence

Impaired Heat Shock Response in Cells Expressing Full-Length Polyglutamine-Expanded Huntingtin

The molecular mechanisms by which polyglutamine (polyQ)-expanded huntingtin (Htt) causes neurodegeneration in Huntington's disease (HD) remain unclear. The malfunction of cellular proteostasis has been suggested as central in HD pathogenesis and also as a target of therapeutic interventions for the treatment of HD. We present results that offer a previously unexplored perspective regarding impaired proteostasis in HD. We find that, under non-stress conditions, the proteostatic capacity of cells expressing full length polyQ-expanded Htt is adequate. Yet, under stress conditions, the presence of polyQ-expanded Htt impairs the heat shock response, a key component of cellular proteostasis. This impaired heat shock response results in a reduced capacity to withstand the damage caused by cellular stress. We demonstrate that in cells expressing polyQ-expanded Htt the levels of heat shock transcription factor 1 (HSF1) are reduced, and, as a consequence, these cells have an impaired a heat shock response. Also, we found reduced HSF1 and HSP70 levels in the striata of HD knock-in mice when compared to wild-type mice. Our results suggests that full length, non-aggregated polyQ-expanded Htt blocks the effective induction of the heat shock response under stress conditions and may thus trigger the accumulation of cellular damage during the course of HD pathogenesis

Genome-Wide Identification of Bcl11b Gene Targets Reveals Role in Brain-Derived Neurotrophic Factor Signaling

B-cell leukemia/lymphoma 11B (Bcl11b) is a transcription factor showing predominant expression in the striatum. To date, there are no known gene targets of Bcl11b in the nervous system. Here, we define targets for Bcl11b in striatal cells by performing chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) in combination with genome-wide expression profiling. Transcriptome-wide analysis revealed that 694 genes were significantly altered in striatal cells over-expressing Bcl11b, including genes showing striatal-enriched expression similar to Bcl11b. ChIP-seq analysis demonstrated that Bcl11b bound a mixture of coding and non-coding sequences that were within 10 kb of the transcription start site of an annotated gene. Integrating all ChIP-seq hits with the microarray expression data, 248 direct targets of Bcl11b were identified. Functional analysis on the integrated gene target list identified several zinc-finger encoding genes as Bcl11b targets, and further revealed a significant association of Bcl11b to brain-derived neurotrophic factor/neurotrophin signaling. Analysis of ChIP-seq binding regions revealed significant consensus DNA binding motifs for Bcl11b. These data implicate Bcl11b as a novel regulator of the BDNF signaling pathway, which is disrupted in many neurological disorders. Specific targeting of the Bcl11b-DNA interaction could represent a novel therapeutic approach to lowering BDNF signaling specifically in striatal cells