Alien Registration- St Laurent, Marie A. (Caribou, Aroostook County)

https://digitalmaine.com/alien_docs/26063/thumbnail.jp

Dark matter RNA: an intelligent scaffold for the dynamic regulation of the nuclear information landscape

Perhaps no other topic in contemporary genomics has inspired such diverse viewpoints as the 95+% of the genome, previously known as “junk DNA,” that does not code for proteins. Here, we present a theory in which dark matter RNA plays a role in the generation of a landscape of spatial micro-domains coupled to the information signaling matrix of the nuclear landscape. Within and between these micro-domains, dark matter RNAs additionally function to tether RNA interacting proteins and complexes of many different types, and by doing so, allow for a higher performance of the various processes requiring them at ultra-fast rates. This improves signal to noise characteristics of RNA processing, trafficking, and epigenetic signaling, where competition and differential RNA binding among proteins drives the computational decisions inherent in regulatory events

Process evaluation of a culturally-tailored physical activity intervention in African-American mother-daughter dyads

The purpose of this study was to describe process evaluation data including intervention fidelity, dosage, quality, participant responsiveness, and program reach for the Mothers And dauGhters daNcing togEther Trial (MAGNET) in Springfield, MA, in Spring 2013 and 2014. Seventy-six mother-daughter dyads were randomized to the mother-daughter group (CH-M, n = 28), the child-only group (CH, n = 25), or the health education group (CON, n = 23). CH-M consisted of 60 min of moderate-to-vigorous culturally-tailored dance classes for dyads. CH consisted of dance classes for the child. All groups received homework tutoring and weekly health newsletters. Process evaluation data were assessed at each intervention session (three days/week, 6-months) with semi-structured questionnaires by researchers. CH dance classes were slightly longer (58.2 ± 3.5 min) than CH-M (54.4 ± 5.5 min). In both groups, participants spent the majority of the dance intervention in light intensity physical activity (PA). Participants in the CH-M group enjoyed participating in MAGNET \u3e 90% of the time. Mothers (92%) indicated that they wanted to continue dance as a form of PA. Mothers expressed that transportation, time commitment, and assessments were barriers to participation. Participants suggested future interventions should include longer intervention length and more communications between research staff and mothers. The MAGNET intervention matched the originally intended program in most aspects. A lower intervention dose was delivered to the CH-M group potentially due to barriers described by mothers. Because mother-daughter interventions have shown minimal effects on increasing PA, it is imperative that researchers utilize process evaluation data to shape future studies

Observations of fresh, anticyclonic eddies in the Hudson Strait outflow

Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Marine Systems 88 (2011): 375-384, doi:10.1016/j.jmarsys.2010.12.004.The waters that flow out through Hudson Strait, a coastal system that connects Hudson Bay with the Labrador Sea, constitute the third largest freshwater contribution to the northern North Atlantic. Recent studies have documented the mean structure and transport of the outflow, as well as highlighting significant variability on synoptic scales (days–week). This study examines the outflow’s variability on these synoptic scales through the use of observations collected by a mooring array from 2005-2006. We focus on the mechanisms that cause the freshwater export to be concentrated in a series of discrete pulses during the fall/winter season. We find that the pulses occur once every 4.4 days on average and are associated with anticyclonic, surface-trapped eddies propagated through the strait by the mean outflow. Their occurrence is related to the passage of storms across Hudson Bay, although local instability processes also play a role in their formation. The eddies are responsible for approximately 40% of the mean volume transport and 50% of the mean freshwater transport out of the strait. We discuss the implications of this freshwater release mechanism on the delivery of nutrient-rich and highly stratified waters to the Labrador shelf, a productive region south of Hudson Strait.This work was funded by National Science Foundation grant OCE-0751554, with additional funding from the Office of Naval Research grant N00014-08-10490

Turbulence and diapycnal mixing over the East Pacific Rise crest near 10°N

Turbulent mixing plays an important role in the return path of the global overturning circulation of the ocean. Previous measurements indicate that much of the mixing takes place near topography, in particular near seamounts and mid-ocean ridges. Here we report on the first microstructure data set collected over the crest and flanks of a fast-spreading ridge. The data indicate that in spite of weak tidally modulated background turbulence levels (ϵ≈10^-10 W kg^-1) diapycnal diffusivity is elevated above 10^-4 m^2 s^-1 below crest depth of the ridge throughout the entire region because of the weak density stratification. Near the peaks and in the narrow deep passages of a chain of seamounts, where large horizontal velocities have been observed, turbulence levels are elevated by up to an order of magnitude above background. We conclude that topographic organization plays an important role in determining spatial patterns of turbulence in this region and that both tidal and subinertial energy contribute to the mixing

Phylogenomics resolves major relationships and reveals significant diversification rate shifts in the evolution of silk moths and relatives

Background: Silkmoths and their relatives constitute the ecologically and taxonomically diverse superfamily Bombycoidea,which includes some of the most charismatic species of Lepidoptera. Despite displaying spectacular forms and diverse ecological traits, relatively little attention has been given to understanding their evolution and drivers of their diversity. To begin to address this problem, we created a new Bombycoidea-specific Anchored Hybrid Enrichment (AHE) probe set and sampled up to 571 loci for 117 taxa across all major lineages of the Bombycoidea, with a newly developed DNA extraction protocol that allows Lepidoptera specimens to be readily sequenced from pinned natural history collections. Results: The well-supported tree was overall consistent with prior morphological and molecular studies, although some taxa were misplaced. The bombycid Arotros Schaus was formally transferred to Apatelodidae. We identified important evolutionary patterns (e.g., morphology, biogeography, and differences in speciation and extinction), and our analysis of diversification rates highlights the stark increases that exist within the Sphingidae (hawkmoths) and Saturniidae (wild silkmoths). Conclusions: Our study establishes a backbone for future evolutionary, comparative, and taxonomic studies of Bombycoidea. We postulate that the rate shifts identified are due to the well-documented bat-moth“arms race”.Our research highlights the flexibility of AHE to generate genomic data from a wide range of museum specimens, both age and preservation method, and will allow researchers to tap into the wealth of biological data residing in natural history collections around the globe

Dissipation processes in the Tongue of the Ocean

Author Posting. © American Geophysical Union, 2016. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Oceans 121 (2016): 3159–3170, doi:10.1002/2015JC011165.The Tongue of the Ocean (TOTO) region located within the Bahamas archipelago is a relatively understudied region in terms of both its biological and physical oceanographic characteristics. A prey-field mapping cruise took place in the fall between 15 September 2008 and 1 October 2008, consisting of a series of transects and “clovers” to study the spatial and temporal variability. The region is characterized by a deep scattering layer (DSL), which is preyed on by nekton that serves as the food for beaked whale and other whale species. This study marks the first of its kind where concurrent measurements of acoustic backscatter and turbulence have been conducted for a nekton scattering layer well below the euphotic zone. Turbulence data collected from a Deep Microstructure Profiler are compared to biological and shear data collected by a 38 kHz Simrad EK 60 echo sounder and a hydrographic Doppler sonar system, respectively. From these measurements, the primary processes responsible for the turbulent production in the TOTO region are assessed. The DSL around 500 m and a surface scattering layer (SSL) are investigated for raised ε values. Strong correlation between turbulence levels and scattering intensity of prey is generally found in the SSL with dissipation levels as large as ∼10−7 W kg−1, 3 orders of magnitude above background levels. In the DSL and during the diel vertical migration, dissipation levels ∼10−8 W kg−1 were observed.U.S. Office of Naval Research Grant Number: N00014-08-1-1162-0

Estuarine Dissolved Organic Carbon Flux From Space: With Application to Chesapeake and Delaware Bays

This study uses a neural network model trained with in situ data, combined with satellite data and hydrodynamic model products, to compute the daily estuarine export of dissolved organic carbon (DOC) at the mouths of Chesapeake Bay (CB) and Delaware Bay (DB) from 2007 to 2011. Both bays show large flux variability with highest fluxes in spring and lowest in fall as well as interannual flux variability (0.18 and 0.27 Tg C/year in 2008 and 2010 for CB; 0.04 and 0.09 Tg C/year in 2008 and 2011 for DB). Based on previous estimates of total organic carbon (TOCexp) exported by all Mid‐Atlantic Bight estuaries (1.2 Tg C/year), the DOC export (CB + DB) of 0.3 Tg C/year estimated here corresponds to 25% of the TOCexp. Spatial and temporal covariations of velocity and DOC concentration provide contributions to the flux, with larger spatial influence. Differences in the discharge of fresh water into the bays (74 billion m3/year for CB and 21 billion m3/year for DB) and their geomorphologies are major drivers of the differences in DOC fluxes for these two systems. Terrestrial DOC inputs are similar to the export of DOC at the bay mouths at annual and longer time scales but diverge significantly at shorter time scales (days to months). Future efforts will expand to the Mid‐Atlantic Bight and Gulf of Maine, and its major rivers and estuaries, in combination with coupled terrestrial‐estuarine‐ocean biogeochemical models that include effects of climate change, such as warming and CO2 increase