Mapping the interstellar medium in galaxies with Herschel/SPIRE

The standard method of mapping the interstellar medium in a galaxy, by observing the molecular gas in the CO 1-0 line and the atomic gas in the 21-cm line, is largely limited with current telescopes to galaxies in the nearby universe. In this letter, we use SPIRE observations of the galaxies M99 and M100 to explore the alternative approach of mapping the interstellar medium using the continuum emission from the dust. We have compared the methods by measuring the relationship between the star-formation rate and the surface density of gas in the galaxies using both methods. We find the two methods give relationships with a similar dispersion, confirming that observing the continuum emission from the dust is a promising method of mapping the interstellar medium in galaxies

Dephasing in Quantum Dots: Quadratic Coupling to Acoustic Phonons

A microscopic theory of optical transitions in quantum dots with carrier-phonon interaction is developed. Virtual transitions into higher confined states with acoustic phonon assistance add a quadratic phonon coupling to the standard linear one, thus extending the independent Boson model. Summing infinitely many diagrams in the cumulant, a numerically exact solution for the interband polarization is found. Its full time dependence and the absorption lineshape of the quantum dot are calculated. It is the quadratic interaction which gives rise to a temperature-dependent broadening of the zero-phonon line, being here calculated for the first time in a consistent scheme.Comment: 4 pages, 2 figure

MSRE-PCR for analysis of gene-specific DNA methylation

Abnormal DNA methylation is observed in certain promoters of neoplastic cells, although the likelihood of methylation for each individual promoter varies. Simultaneous analysis of many promoters in the same sample can allow use of statistical methods for identification of neoplasia. Here we describe an assay for such analysis, based on digestion of genomic DNA with methylation-sensitive restriction enzyme and multiplexed PCR with gene-specific primers (MSRE-PCR). MSRE-PCR includes extensive digestion of genomic DNA (uncut fragments cannot be identified by PCR), can be applied to dilute samples (<1 pg/μl), requires limited amount of starting material (42 pg or genomic equivalent of seven cells) and can identify methylation in a heterogeneous mix containing <2% of cells with methylated fragments. When applied to 53 promoters of breast cancer cell lines MCF-7, MDA-MB-231 and T47D, MSRE-PCR correctly identified the methylation status of genes analyzed by other techniques. For selected genes results of MSRE-PCR were confirmed by methylation-specific PCR and bisulfite sequencing. The assay can be configured for any number of desired targets in any user-defined set of genes

Ultraslow light propagation in an inhomogeneously broadened rare-earth ion-doped crystal

We show that Coherent Population Oscillations effect allows to burn a narrow spectral hole (26Hz) within the homogeneous absorption line of the optical transition of an Erbium ion-doped crystal. The large dispersion of the index of refraction associated with this hole permits to achieve a group velocity as low as 2.7m/s with a ransmission of 40%. We especially benefit from the inhomogeneous absorption broadening of the ions to tune both the transmission coefficient, from 40% to 90%, and the light group velocity from 2.7m/s to 100m/s

Label-free detection of anticancer drug paclitaxel in living cells by confocal Raman microscopy

Confocal Raman microscopy, a non-invasive, label-free, and high spatial resolution imaging technique is employed to trace the anticancer drug paclitaxel in living Michigan Cancer Foundation-7 (MCF-7) cells. The Raman images were treated by K-mean cluster analysis to detect the drug in cells. Distribution of paclitaxel in cells is verified by calculating the correlation coefficient between the reference spectrum of the drug and the whole Raman image spectra. A time dependent gradual diffusion of paclitaxel all over the cell is observed suggesting a complementary picture of the pharmaceutical action of this drug based on rapid binding of free tubulin to crystallized paclitaxel. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4794871

Subaru Spectroscopy and Spectral Modeling of Cygnus A

We present high angular resolution ($\sim$0.5$^\prime$$^\prime$) MIR spectra of the powerful radio galaxy, Cygnus A, obtained with the Subaru telescope. The overall shape of the spectra agree with previous high angular resolution MIR observations, as well as previous Spitzer spectra. Our spectra, both on and off nucleus, show a deep silicate absorption feature. The absorption feature can be modeled with a blackbody obscured by cold dust or a clumpy torus. The deep silicate feature is best fit by a simple model of a screened blackbody, suggesting foreground absorption plays a significant, if not dominant role, in shaping the spectrum of Cygnus A. This foreground absorption prevents a clear view of the central engine and surrounding torus, making it difficult to quantify the extent the torus attributes to the obscuration of the central engine, but does not eliminate the need for a torus in Cygnus A

Scanning Electron Microscopy Study of Particles Generated by Laser Damage of Aluminum Surfaces

A high intensity iodine laser (1.315 μm wavelength) was used to study laser-surface damage in vacuum. A 22mm diameter laser beam containing 3.9 J of energy was focused to a 1 mm spot on an aluminum plate mounted in a vacuum chamber. The laser pulse width was 7 μs (FWHM). A copper strip mounted on a quarter circle plate 3.5 cm from the laser spot was used to collect particles ejected from the surface. A scanning electron microscope (SEM) was used to measure the size and spatial distribution of the particles collected on the copper strip. It was found that aluminum droplets were ejected from the crater formed by the laser pulse. The particle sizes ranged from 0.25 to 45 μm in diameter. The peak of the particle size distribution occurred at 1 μm diameter. The maximum spatial distribution of droplets occurred at near 47° from the normal to the target surface. Measurement of the mass lost by the target plate and the volume of aluminum removed from the craters showed that most of the aluminum ejected from the craters remained on the target surface. SEM examination of the surface adjacent to the craters showed that most of the ejected aluminum was liquid splattered around the crater. The particle size and spatial distributions are considered preliminary because droplets smaller than 0.25 μm could not be detected and the copper collector strip was examined in only six areas corresponding to six emission angles