14 research outputs found
A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction
Background: Carbohydrates play a major role in cell signaling in many biological processes. We have developed a set of glycomimetic drugs that mimic the structure of carbohydrates and represent a novel source of therapeutics for endothelial dysfunction, a key initiating factor in cardiovascular complications. Purpose: Our objective was to determine the protective effects of small molecule glycomimetics against free fatty acidinduced endothelial dysfunction, focusing on nitric oxide (NO) and oxidative stress pathways. Methods: Four glycomimetics were synthesized by the stepwise transformation of 2,5dihydroxybenzoic acid to a range of 2,5substituted benzoic acid derivatives, incorporating the key sulfate groups to mimic the interactions of heparan sulfate. Endothelial function was assessed using acetylcholineinduced, endotheliumdependent relaxation in mouse thoracic aortic rings using wire myography. Human umbilical vein endothelial cell (HUVEC) behavior was evaluated in the presence or absence of the free fatty acid, palmitate, with or without glycomimetics (1µM). DAF2 and H2DCFDA assays were used to determine nitric oxide (NO) and reactive oxygen species (ROS) production, respectively. Lipid peroxidation colorimetric and antioxidant enzyme activity assays were also carried out. RTPCR and western blotting were utilized to measure Akt, eNOS, Nrf2, NQO1 and HO1 expression. Results: Ex vivo endotheliumdependent relaxation was significantly improved by the glycomimetics under palmitateinduced oxidative stress. In vitro studies showed that the glycomimetics protected HUVECs against the palmitateinduced oxidative stress and enhanced NO production. We demonstrate that the protective effects of preincubation with glycomimetics occurred via upregulation of Akt/eNOS signaling, activation of the Nrf2/ARE pathway, and suppression of ROSinduced lipid peroxidation. Conclusion: We have developed a novel set of small molecule glycomimetics that protect against free fatty acidinduced endothelial dysfunction and thus, represent a new category of therapeutic drugs to target endothelial damage, the first line of defense against cardiovascular disease
Grain size composition of metalliferous sediments from the East Pacific Rise axial zone at 21°S
It is shown that a laser granulometric analysis makes it possible to obtain adequate results with respect to grain size composition of metalliferous sediments in contrast to water-mechanical types of analysis. Metalliferous sediments of the studied region are usually represented by pelitic (<0.01 mm) oozes, less frequently by silty-pelitic and fine silt oozes
Optimization of formulations with balanced biochemical composition and possibilities for their extrusion
Abstract. Combinations of different raw materials (beans, einkorn wheat, and buckwheat) for obtaining formulations with high protein content and balanced
amino acid composition were studied using simplex centroid design. The target functions for optimization were content of protein, sulfur-containing amino acid
methionine and cysteine, lysine, and tryptophan. The optimal area of combinations of raw materials in the food formulas with balanced biochemical
composition was obtained. Optimized ternary mixture consisting of 50% bean, 40% einkorn wheat, and 10% buckwheat with different moisture content (16, 22,
and 28%) has been extruded in a laboratory single screw extruder (Brabender 20 DN, Germany). Extrusion parameters were as follows: feed screw speed 50
rpm, die diameter 3 mm, screw compression ratio 2:1, temperature profile 100/140/160ºC, screw speed 160 rpm. The three extrudates with different initial
moisture were evaluated by sectional expansion index, water absorption index, water solubility index, and density, with the aim of choosing the best treatment.
The results demonstrated that the 22% initial moisture content yielded an extrudate with good physicochemical characteristics overall but an optimization study
is needed to confirm this